CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 3
extracellular 4
endoplasmic reticulum 5
vacuole 4
plasma membrane 6
golgi 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	mitochondrion
Any Predictor:	plastid, secretory
BaCelLo:	plastid
ChloroP:	plastid
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	plastid
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

mitochondrion: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH11076
KRH21197
KRH42923
KRH42926
KRH45251
KRH59035
KRH59036

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G46890.1	KRH11076	AT4G19030.1	21798944
AT2G46890.1	KRH21197	AT4G19030.1	21798944
AT2G46890.1	KRH42923	AT4G19030.1	21798944
AT2G46890.1	KRH42926	AT4G19030.1	21798944
AT2G46890.1	KRH45251	AT4G19030.1	21798944
AT2G46890.1	KRH59035	AT4G19030.1	21798944
AT2G46890.1	KRH59036	AT4G19030.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH47737	Soybean	plasma membrane	76.66	74.77
KRH06317	Soybean	plasma membrane	76.34	74.46
VIT_15s0048g02220.t01	Wine grape	plasma membrane	70.98	70.09
Solyc02g063390.2.1	Tomato	plasma membrane	65.93	65.72
PGSC0003DMT400026984	Potato	plasma membrane	65.93	65.72
CDX80055	Canola	plasma membrane	64.35	62.96
Bra004499.1-P	Field mustard	plasma membrane	64.04	62.65
HORVU2Hr1G093180.1	Barley	golgi, mitochondrion, plasma membrane	41.01	62.5
AT2G46890.1	Thale cress	plasma membrane	63.41	62.42
TraesCS2B01G051300.1	Wheat	plasma membrane	61.51	60.94
TraesCS2D01G037000.1	Wheat	plasma membrane	60.88	59.75
TraesCS2A01G038000.1	Wheat	plasma membrane	60.88	59.2
EES10337	Sorghum	plasma membrane	59.31	58.75
GSMUA_Achr1P00860_001	Banana	cytosol, endoplasmic reticulum, peroxisome	64.35	56.98
Solyc01g005320.2.1	Tomato	plasma membrane	57.1	56.21
PGSC0003DMT400051424	Potato	plasma membrane	56.78	55.9
HORVU2Hr1G005680.1	Barley	endoplasmic reticulum, plasma membrane	59.94	54.6
Zm00001d024925_P001	Maize	plasma membrane	60.25	54.42
Os04t0105300-01	Rice	cytosol, nucleus, plastid	41.96	46.34
KRH41812	Soybean	endoplasmic reticulum, plasma membrane, vacuole	22.71	24.74
KRH60513	Soybean	plasma membrane	22.71	23.53

Protein Annotations

Gene3D:1.20.120.1630	EntrezGene:100807179	InterPro:3-oxo-5_a-steroid_4-DH_C	MapMan:35.2	EMBL:ACUP02012181	EMBL:BT095529
UniProt:C6TD75	InterPro:DUF1295	EnsemblPlantsGene:GLYMA_19G261600	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006629	GO:GO:0008150	GO:GO:0008152
GO:GO:0016020	GO:GO:0016021	GO:GO:0016627	GO:GO:0055114	InterPro:IPR001104	EnsemblPlants:KRG97275
ProteinID:KRG97275	ProteinID:KRG97275.1	PFAM:PF06966	PFscan:PS50244	PANTHER:PTHR32251	PANTHER:PTHR32251:SF14
TMHMM:TMhelix	UniParc:UPI0001B16549	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr19:-:50484609..50486110

Molecular Weight (calculated)

37660.6 Da

IEP (calculated)

9.728

GRAVY (calculated)

0.163

Length

317 amino acids

Sequence
(BLAST)

001: MGSVSYSNLK NAMIAFLVPL PSILFYLSFQ SAISTPSSWS SLWTWCYHHP LLLANVLFFF NVNFLFWLIG LIQSSHWMID PYWTVIPVML VHYYSTHPLA
101: QYQWWRSRIV TLLTWAWSVR LIHNYFRREK WQWGAREDWR FTDLSHRYGR HWWWASFFAI YVPQQVFLIG LSLPFYVIHS VNQPLSMWDL VAIVVCVSGI
201: VTAYIADTQL YNFVSRKNKE VPILDKGLWY YSRHPNYFGE QVWWWGMAVF AWNLGHGWAF IGALANTMCL AYVTKLVEDR MLKQDSRAEA FRLYQKTTSL
301: WIPWFKSSPL GLKSKDA

Best Arabidopsis Sequence Match ( AT2G46890.1 )
(BLAST)

001: MNRNLRNAIV AFLAPLPSIV FYLTFLSNYS SSSDSELSSI QSWCLNHPLL LANLLFFLNV NVLFWVIGLL QSSHWMIDVY WTVIPVMLVH YFASHPLSQY
101: NKLRSMIVIT LTWIWSIRLT HNYFRRENWE WGAREDWRFN DLRKQYGKHW WWLSFFSVYV SQQIFLIGIC LPLYVIHSID EPLNIWDFIS SAICLTGIVM
201: AYFADTQLHE FVTGNQKLKE QGKPKIPNLD SGLWRYSRHP NYLGEQLWWW GLVIFAWNLG QGWTLIGALV NTLCLVYVTI LVERRMVKQQ YRAEAYRAYQ
301: KTTSVWIPWF KSHAAATKDK NT

Arabidopsis Description

3-oxo-5-alpha-steroid 4-dehydrogenase (DUF1295) [Source:UniProtKB/TrEMBL;Acc:O81042]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.