CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

plastid 2
extracellular 6
endoplasmic reticulum 4
vacuole 4
plasma membrane 6
golgi 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	plastid, secretory
BaCelLo:	plastid
EpiLoc:	extracellular
iPSORT:	secretory
MultiLoc:	extracellular
Plant-mPloc:	plastid
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH11076
KRH21197
KRH42923
KRH42926
KRH45251
KRH59035
KRH59036

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G46890.1	KRH11076	AT4G19030.1	21798944
AT2G46890.1	KRH21197	AT4G19030.1	21798944
AT2G46890.1	KRH42923	AT4G19030.1	21798944
AT2G46890.1	KRH42926	AT4G19030.1	21798944
AT2G46890.1	KRH45251	AT4G19030.1	21798944
AT2G46890.1	KRH59035	AT4G19030.1	21798944
AT2G46890.1	KRH59036	AT4G19030.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH06317	Soybean	plasma membrane	94.46	94.46
KRG97275	Soybean	mitochondrion	74.77	76.66
VIT_15s0048g02220.t01	Wine grape	plasma membrane	71.08	71.96
PGSC0003DMT400026984	Potato	plasma membrane	67.69	69.18
AT2G46890.1	Thale cress	plasma membrane	67.69	68.32
HORVU2Hr1G093180.1	Barley	golgi, mitochondrion, plasma membrane	43.69	68.27
Solyc02g063390.2.1	Tomato	plasma membrane	66.77	68.24
CDX80055	Canola	plasma membrane	67.08	67.28
Bra004499.1-P	Field mustard	plasma membrane	66.77	66.98
TraesCS2B01G051300.1	Wheat	plasma membrane	64.0	65.0
TraesCS2A01G038000.1	Wheat	plasma membrane	64.62	64.42
TraesCS2D01G037000.1	Wheat	plasma membrane	63.38	63.78
EES10337	Sorghum	plasma membrane	62.46	63.44
GSMUA_Achr1P00860_001	Banana	cytosol, endoplasmic reticulum, peroxisome	66.15	60.06
HORVU2Hr1G005680.1	Barley	endoplasmic reticulum, plasma membrane	64.0	59.77
Zm00001d024925_P001	Maize	plasma membrane	62.46	57.83
Solyc01g005320.2.1	Tomato	plasma membrane	56.92	57.45
PGSC0003DMT400051424	Potato	plasma membrane	56.0	56.52
Os04t0105300-01	Rice	cytosol, nucleus, plastid	45.85	51.92
KRH41812	Soybean	endoplasmic reticulum, plasma membrane, vacuole	22.46	25.09
KRH60513	Soybean	plasma membrane	22.46	23.86

Protein Annotations

Gene3D:1.20.120.1630	EntrezGene:100783556	InterPro:3-oxo-5_a-steroid_4-DH_C	MapMan:35.2	EMBL:ACUP02004162	InterPro:DUF1295
EnsemblPlantsGene:GLYMA_07G047400	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0006629	GO:GO:0008150	GO:GO:0008152	GO:GO:0016020	GO:GO:0016021
GO:GO:0016627	GO:GO:0055114	UniProt:I1KHL1	InterPro:IPR001104	EnsemblPlants:KRH47737	ProteinID:KRH47737
ProteinID:KRH47737.1	PFAM:PF06966	PFscan:PS50244	PANTHER:PTHR32251	PANTHER:PTHR32251:SF14	TMHMM:TMhelix
UniParc:UPI0002337BFA	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr7:+:3977971..3979901

Molecular Weight (calculated)

38638.8 Da

IEP (calculated)

9.640

GRAVY (calculated)

0.170

Length

325 amino acids

Sequence
(BLAST)

001: MASGRNLKNA VIAFLVPLPS IFFYLSFLNH YDSANSPLFW STLWSWCYHH PLLLANALFF LNVNVLFWAI GLIQSSHWMI DPYWTVIPVM LVHYYATHPL
101: AHYDWWRSRI VILLTWVWSA RLTHNYFRRE RWQWGAREDW RFTEMSQQYG KQWWWVSFFA VYVSQQMFLI ALSLPLYAVH TFNEPLSMWD LVATVVCLCG
201: IVIAYFADTQ LYEFVSRNNK LKGLGKPVVF VLDSGLWYYC RHPNYFGEQL WWWGLVVFAW NLGHGWTFIG ALVNTMCLAY VTRLVEDRML KQKSRAEAFR
301: VYQKKTSVWV PWFKSSPSGV KNKNA

Best Arabidopsis Sequence Match ( AT2G46890.1 )
(BLAST)

001: MNRNLRNAIV AFLAPLPSIV FYLTFLSNYS SSSDSELSSI QSWCLNHPLL LANLLFFLNV NVLFWVIGLL QSSHWMIDVY WTVIPVMLVH YFASHPLSQY
101: NKLRSMIVIT LTWIWSIRLT HNYFRRENWE WGAREDWRFN DLRKQYGKHW WWLSFFSVYV SQQIFLIGIC LPLYVIHSID EPLNIWDFIS SAICLTGIVM
201: AYFADTQLHE FVTGNQKLKE QGKPKIPNLD SGLWRYSRHP NYLGEQLWWW GLVIFAWNLG QGWTLIGALV NTLCLVYVTI LVERRMVKQQ YRAEAYRAYQ
301: KTTSVWIPWF KSHAAATKDK NT

Arabidopsis Description

3-oxo-5-alpha-steroid 4-dehydrogenase (DUF1295) [Source:UniProtKB/TrEMBL;Acc:O81042]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.