Factsheet for Bra004499.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

plastid 2
extracellular 4
endoplasmic reticulum 4
vacuole 4
plasma membrane 7
golgi 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	plastid, secretory
BaCelLo:	plastid
iPSORT:	secretory
MultiLoc:	plasma membrane
Plant-mPloc:	plastid
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra012567.1-P
Bra013361.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G46890.1	Bra012567.1-P	AT4G19030.1	21798944
AT2G46890.1	Bra013361.1-P	AT4G19030.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDX80055	Canola	plasma membrane	99.38	99.38
AT2G46890.1	Thale cress	plasma membrane	87.96	88.51
HORVU2Hr1G093180.1	Barley	golgi, mitochondrion, plasma membrane	43.83	68.27
KRH06317	Soybean	plasma membrane	67.9	67.69
Solyc02g063390.2.1	Tomato	plasma membrane	66.36	67.61
VIT_15s0048g02220.t01	Wine grape	plasma membrane	66.67	67.29
KRH47737	Soybean	plasma membrane	66.98	66.77
PGSC0003DMT400026984	Potato	plasma membrane	65.12	66.35
KRG97275	Soybean	mitochondrion	62.65	64.04
TraesCS2D01G037000.1	Wheat	plasma membrane	63.58	63.78
TraesCS2B01G051300.1	Wheat	plasma membrane	62.96	63.75
EES10337	Sorghum	plasma membrane	62.65	63.44
TraesCS2A01G038000.1	Wheat	plasma membrane	63.58	63.19
GSMUA_Achr1P00860_001	Banana	cytosol, endoplasmic reticulum, peroxisome	64.81	58.66
HORVU2Hr1G005680.1	Barley	endoplasmic reticulum, plasma membrane	62.96	58.62
PGSC0003DMT400051424	Potato	plasma membrane	57.1	57.45
Solyc01g005320.2.1	Tomato	plasma membrane	56.79	57.14
Zm00001d024925_P001	Maize	plasma membrane	61.11	56.41
Os04t0105300-01	Rice	cytosol, nucleus, plastid	47.53	53.66
Bra015977.1-P	Field mustard	plasma membrane	22.53	25.09
Bra008106.1-P	Field mustard	endoplasmic reticulum, plasma membrane, vacuole	21.3	23.55
Bra025926.1-P	Field mustard	plasma membrane	20.99	23.29

Protein Annotations

Gene3D:1.20.120.1630	InterPro:3-oxo-5_a-steroid_4-DH_C	MapMan:35.2	EnsemblPlantsGene:Bra004499	EnsemblPlants:Bra004499.1	EnsemblPlants:Bra004499.1-P
InterPro:DUF1295	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0006629	GO:GO:0008150	GO:GO:0008152	GO:GO:0016020	GO:GO:0016021
GO:GO:0016627	GO:GO:0055114	InterPro:IPR001104	UniProt:M4CJW5	PFAM:PF06966	PFscan:PS50244
PANTHER:PTHR32251	PANTHER:PTHR32251:SF14	TMHMM:TMhelix	UniParc:UPI00025472D2	SEG:seg	:

Description

AT2G46890 (E=2e-170) | oxidoreductase, acting on the CH-CH group of donors

Coordinates

chrA05:+:456751..457909

Molecular Weight (calculated)

38447.6 Da

IEP (calculated)

9.304

GRAVY (calculated)

0.111

Length

324 amino acids

Sequence
(BLAST)

001: MNRNLRNAII AFLAPLPSIV FYLSFLRSYS PTSDPELSHI HSWCSNHPLL LANLLFFLNV NFLFWVIGLL QSSHWMIDVY WTVIPVMLVH YFASHPLGEY
101: NKWRSMVVVI LTWVWSVRLT HNYFRRENWE WGAREDWRFN DLRKQYGKHW WWLSFFSVYV SQQIFLIGIC LPLYIIHSVD APLNIWDFVS SAICLTGIVM
201: AYFADTQLHE FVTQNQKLKE QGKPKIPNLD TGLWYYSRHP NYLGEQLWWW GLVVFSWNLG QGWTLIGALV NTLCLVYVTI LVERRMVKQE YRAEAYRAYQ
301: KTTSVWIPWF KSQVAAAVSK DKST

Best Arabidopsis Sequence Match ( AT2G46890.1 )
(BLAST)

001: MNRNLRNAIV AFLAPLPSIV FYLTFLSNYS SSSDSELSSI QSWCLNHPLL LANLLFFLNV NVLFWVIGLL QSSHWMIDVY WTVIPVMLVH YFASHPLSQY
101: NKLRSMIVIT LTWIWSIRLT HNYFRRENWE WGAREDWRFN DLRKQYGKHW WWLSFFSVYV SQQIFLIGIC LPLYVIHSID EPLNIWDFIS SAICLTGIVM
201: AYFADTQLHE FVTGNQKLKE QGKPKIPNLD SGLWRYSRHP NYLGEQLWWW GLVIFAWNLG QGWTLIGALV NTLCLVYVTI LVERRMVKQQ YRAEAYRAYQ
301: KTTSVWIPWF KSHAAATKDK NT

Arabidopsis Description

3-oxo-5-alpha-steroid 4-dehydrogenase (DUF1295) [Source:UniProtKB/TrEMBL;Acc:O81042]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.