CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 2
cytosol 4
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, nucleus, secretory
BaCelLo:	nucleus
EpiLoc:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	cytosol
PProwler:	secretory
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH08348
KRH28553
KRH69993
KRH76911

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G56010.1	KRH08348	AT5G66570.1	16619024
AT5G56010.1	KRH28553	AT5G66570.1	16619024
AT5G56010.1	KRH69993	AT5G66570.1	16619024
AT5G56010.1	KRH76911	AT5G66570.1	16619024

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH46427	Soybean	nucleus	98.15	98.57
VIT_00s0194g00030.t01	Wine grape	cytosol	95.87	96.28
KRH14196	Soybean	endoplasmic reticulum, nucleus	94.59	94.86
KRH73936	Soybean	endoplasmic reticulum, nucleus	94.3	94.3
VIT_19s0015g01090.t01	Wine grape	cytosol	94.16	93.89
Solyc12g015880.1.1	Tomato	nucleus	93.45	93.85
PGSC0003DMT400076601	Potato	cytosol	93.16	93.56
Bra035593.1-P	Field mustard	cytosol	89.32	93.3
PGSC0003DMT400037336	Potato	cytosol	92.88	93.28
Solyc07g065840.2.1	Tomato	nucleus, plastid	92.73	93.13
PGSC0003DMT400057003	Potato	cytosol	92.73	93.13
Bra035599.1-P	Field mustard	cytosol	74.64	93.07
CDY11961	Canola	cytosol	48.15	92.86
AT5G56010.1	Thale cress	cytosol	92.17	92.56
Bra028924.1-P	Field mustard	cytosol	92.17	92.56
Bra028952.1-P	Field mustard	cytosol	92.17	92.56
CDY11967	Canola	cytosol	75.78	92.36
Bra028948.1-P	Field mustard	cytosol	91.45	91.98
AT5G56000.1	Thale cress	cytosol	90.74	91.13
CDY22598	Canola	cytosol	61.11	90.32
AT5G56030.2	Thale cress	cytosol	92.45	89.15
KRH38373	Soybean	nucleus	88.46	88.84
KRH08858	Soybean	nucleus	87.89	88.27
CDY36807	Canola	cytosol	72.22	88.17
CDY64982	Canola	cytosol	68.95	88.16
CDY06551	Canola	cytosol	71.94	88.13
CDY20742	Canola	cytosol	69.09	88.02
CDY36775	Canola	cytosol	18.38	87.76
CDY11921	Canola	cytosol	67.09	87.71
KRH33083	Soybean	cytosol	34.9	87.19
CDY36771	Canola	cytosol	18.23	87.07
CDY11962	Canola	cytosol	17.81	86.21
CDY36776	Canola	cytosol	44.44	85.95
CDY36772	Canola	cytosol	44.44	85.95
KRG89657	Soybean	cytosol	13.39	84.68
KRH04730	Soybean	cytosol	43.02	83.89
KRH41482	Soybean	mitochondrion	75.78	81.22
KRH05319	Soybean	endoplasmic reticulum	35.04	76.16
KRG94627	Soybean	cytosol	29.06	65.59
KRH50203	Soybean	cytosol	24.64	56.91
KRH05964	Soybean	endoplasmic reticulum	51.0	43.98
KRH17444	Soybean	nucleus	51.42	42.62
KRH71021	Soybean	nucleus	46.58	41.18
KRH75182	Soybean	plastid	46.15	40.86
KRH14117	Soybean	mitochondrion	45.16	39.77
KRH74006	Soybean	mitochondrion	44.73	39.7

Protein Annotations

Gene3D:1.20.120.790	EntrezGene:100194423	MapMan:19.1.6.1	Gene3D:3.30.230.80	Gene3D:3.30.565.10	Gene3D:3.30.70.2140
Gene3D:3.40.50.11260	EMBL:ACUP02010889	ncoils:Coil	UniProt:D6C4Z9	EMBL:FJ969430	EnsemblPlantsGene:GLYMA_18G074100
GO:GO:0000166	GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0006457
GO:GO:0006950	GO:GO:0008150	GO:GO:0009987	GO:GO:0051082	InterPro:HATPase_C	InterPro:HATPase_C_sf
InterPro:HSP90_C	InterPro:Heat_shock_protein_90_CS	InterPro:Hsp90_N	InterPro:Hsp90_fam	InterPro:IPR036890	InterPro:IPR037196
EnsemblPlants:KRG98439	ProteinID:KRG98439	ProteinID:KRG98439.1	HAMAP:MF_00505	PFAM:PF00183	PFAM:PF02518
PIRSF:PIRSF002583	PRINTS:PR00775	ScanProsite:PS00298	PANTHER:PTHR11528	PANTHER:PTHR11528:SF74	InterPro:Ribosomal_S5_D2-typ_fold
SMART:SM00387	SUPFAM:SSF110942	SUPFAM:SSF54211	SUPFAM:SSF55874	UniParc:UPI0001D26941	SEG:seg

Description

hypothetical protein

Coordinates

chr18:+:7029009..7032141

Molecular Weight (calculated)

80384.6 Da

IEP (calculated)

4.657

GRAVY (calculated)

-0.579

Length

702 amino acids

Sequence
(BLAST)

001: MAETETFAFQ AEINQLLSLI INTFYSNKEI FLRELISNAS DALDKIRFES LTDKSKLDAQ PELFIHIIPD KTNNSLTIVD SGIGMTKADL VNNLGTIARS
101: GTKEFMEALA AGADVSMIGQ FGVGFYSAYL VAEKVIVTSK HNDDEQYVWE SQAGGSFTVT RDTSGEVLGR GTKITLFLKE DQLEYLEERR LKDLIKKHSE
201: FISYPISLWV EKTTEKEISD DEDEEEKKDE EGKVEDVDEE KEKEEKKKKK IKEVSHEWSL VNKQKPIWMR KPEEITKEEY SAFYKSLTND WEEHLAVKHF
301: SVEGQLEFKA ILFVPKRAPF DLFDTRKKPN NIKLYVRRVF IMDNCEELIP EYLGFVKGIV DSEDLPLNIS REMLQQNKIL KVIRKNLVKK CLELFFEIAE
401: NKEDYNKFYE AFSKNLKLGI HEDSQNKGKI AELLRYHSTK SGDELTSLKD YVTRMKEGQS DIYYITGESK KAVENSPFLE KLKKKGYEVL FMVDAIDEYA
501: VGQLKEFEGK KLVSATKEGL KLDESEDEKK KQEELKEKFD NLCKVIKDVL GDKVEKVVVS DRVVDSPCCL VTGEYGWTAN MERIMKAQAL RDNSMAGYMS
601: SKKTMEINPE NPIMEELRKR ADADKNDKSV KDLVLLLFET ALLTSGFSLD EPNTFGNRIH RMLKLGLSID EDAAEADADA DMPPLEEEAE ADAEGSKMEE
701: VD

Best Arabidopsis Sequence Match ( AT5G52640.1 )
(BLAST)

001: MADAETFAFQ AEINQLLSLI INTFYSNKEI FLRELISNSS DALDKIRFES LTDKSKLDGQ PELFIRLVPD KSNKTLSIID SGIGMTKADL VNNLGTIARS
101: GTKEFMEALQ AGADVSMIGQ FGVGFYSAYL VAEKVVVTTK HNDDEQYVWE SQAGGSFTVT RDVDGEPLGR GTKITLFLKD DQLEYLEERR LKDLVKKHSE
201: FISYPIYLWT EKTTEKEISD DEDEDEPKKE NEGEVEEVDE EKEKDGKKKK KIKEVSHEWE LINKQKPIWL RKPEEITKEE YAAFYKSLTN DWEDHLAVKH
301: FSVEGQLEFK AILFVPKRAP FDLFDTRKKL NNIKLYVRRV FIMDNCEELI PEYLSFVKGV VDSDDLPLNI SRETLQQNKI LKVIRKNLVK KCIEMFNEIA
401: ENKEDYTKFY EAFSKNLKLG IHEDSQNRGK IADLLRYHST KSGDEMTSFK DYVTRMKEGQ KDIFYITGES KKAVENSPFL ERLKKRGYEV LYMVDAIDEY
501: AVGQLKEYDG KKLVSATKEG LKLEDETEEE KKKREEKKKS FENLCKTIKE ILGDKVEKVV VSDRIVDSPC CLVTGEYGWT ANMERIMKAQ ALRDSSMSGY
601: MSSKKTMEIN PDNGIMEELR KRAEADKNDK SVKDLVMLLY ETALLTSGFS LDEPNTFAAR IHRMLKLGLS IDEDENVEED GDMPELEEDA AEESKMEEVD

Arabidopsis Description

HSP90-1Heat shock protein 90-1 [Source:UniProtKB/Swiss-Prot;Acc:P27323]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.