CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 1
cytosol 5
extracellular 2
endoplasmic reticulum 2
vacuole 2
plasma membrane 2
golgi 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, nucleus, secretory
BaCelLo:	nucleus
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	cytosol
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
mitochondrion: 27780359

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91395
KRG94038
KRH05436
KRH08815
KRH15573
KRH23877
KRH27885
KRH35228
KRH35422
KRH38332
KRH39924
KRH61515
KRH77588

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G52640.1	KRH05436	AT2G26150.1	19366428
AT5G52640.1	KRH15573	AT2G26150.1	19366428
AT5G52640.1	KRH61515	AT2G26150.1	19366428
AT5G52640.1	KRH23877	AT3G21640.1	12410806
AT5G52640.1	KRH39924	AT3G21640.1	12410806
AT5G52640.1	KRG94038	AT4G11260.1	18065690
AT5G52640.1	KRH08815	AT4G11260.1	18065690
AT5G52640.1	KRH27885	AT4G11260.1	18065690
AT5G52640.1	KRH35228	AT4G11260.1	18065690
AT5G52640.1	KRH38332	AT4G11260.1	18065690
AT5G52640.1	KRH77588	AT4G11260.1	18065690
AT5G52640.1	KRG91395	AT5G51700.1	18065690
AT5G52640.1	KRH35422	AT5G51700.1	18065690

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH08858	Soybean	nucleus	98.71	98.71
Solyc03g007890.2.1	Tomato	cytosol, extracellular, nucleus, plastid	93.85	93.85
VIT_02s0025g00280.t01	Wine grape	cytosol	93.99	93.46
CDY27839	Canola	cytosol	93.99	93.19
PGSC0003DMT400024594	Potato	cytosol	93.85	93.18
AT5G52640.1	Thale cress	cytosol	93.28	93.14
VIT_16s0050g01150.t01	Wine grape	cytosol	93.56	92.9
GSMUA_Achr8P19130_001	Banana	cytosol	77.11	92.61
KRH14196	Soybean	endoplasmic reticulum, nucleus	88.84	88.71
KRH46427	Soybean	nucleus	88.7	88.7
KRG98439	Soybean	nucleus	88.84	88.46
KRH73936	Soybean	endoplasmic reticulum, nucleus	88.41	88.03
Zm00001d024903_P001	Maize	plasma membrane	89.84	87.96
TraesCS2A01G033700.1	Wheat	plastid	88.84	87.84
EES11741	Sorghum	cytosol	89.84	87.71
TraesCS2D01G033200.2	Wheat	golgi	89.13	87.5
TraesCS2B01G047400.1	Wheat	cytosol	88.7	87.08
Os04t0107900-02	Rice	cytosol	30.76	86.35
Bra003119.1-P	Field mustard	plastid	74.39	85.53
CDY50297	Canola	plastid	76.11	83.65
KRH33083	Soybean	cytosol	33.33	82.92
KRG89657	Soybean	cytosol	12.73	80.18
KRH04730	Soybean	cytosol	40.63	78.89
KRH41482	Soybean	mitochondrion	71.1	75.88
KRH05319	Soybean	endoplasmic reticulum	33.91	73.37
KRG94627	Soybean	cytosol	28.76	64.63
KRH50203	Soybean	cytosol	26.47	60.86
KRH05964	Soybean	endoplasmic reticulum	51.79	44.47
KRH17444	Soybean	nucleus	52.36	43.21
KRH71021	Soybean	nucleus	46.64	41.06
KRH75182	Soybean	plastid	46.07	40.61
KRH14117	Soybean	mitochondrion	46.07	40.4
KRH74006	Soybean	mitochondrion	45.49	40.2

Protein Annotations

Gene3D:1.20.120.790	EntrezGene:100811234	MapMan:19.1.6.1	Gene3D:3.30.230.80	Gene3D:3.30.565.10	Gene3D:3.30.70.2140
Gene3D:3.40.50.11260	EMBL:ACUP02005803	ncoils:Coil	EnsemblPlantsGene:GLYMA_09G131500	GO:GO:0000166	GO:GO:0003674
GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0006457	GO:GO:0006950	GO:GO:0008150
GO:GO:0009987	GO:GO:0051082	InterPro:HATPase_C	InterPro:HATPase_C_sf	InterPro:HSP90_C	InterPro:Heat_shock_protein_90_CS
InterPro:Hsp90_N	InterPro:Hsp90_fam	UniProt:I1L314	InterPro:IPR036890	InterPro:IPR037196	EnsemblPlants:KRH38373
ProteinID:KRH38373	ProteinID:KRH38373.1	EMBL:KT726857	HAMAP:MF_00505	PFAM:PF00183	PFAM:PF02518
PIRSF:PIRSF002583	PRINTS:PR00775	ScanProsite:PS00298	PANTHER:PTHR11528	PANTHER:PTHR11528:SF74	InterPro:Ribosomal_S5_D2-typ_fold
SMART:SM00387	SUPFAM:SSF110942	SUPFAM:SSF54211	SUPFAM:SSF55874	UniParc:UPI00023385F4	SEG:seg

Description

hypothetical protein

Coordinates

chr9:-:32754514..32758018

Molecular Weight (calculated)

80393.5 Da

IEP (calculated)

4.686

GRAVY (calculated)

-0.646

Length

699 amino acids

Sequence
(BLAST)

001: MADAETFAFQ AEINQLLSLI INTFYSNKEI FLRELISNAS DALDKIRFES LTDKSKLDAQ PELFIRLVPD KDNKTLSIID SGIGMTKADL VNNLGTIARS
101: GTKEFMEALQ AGADVSMIGQ FGVGFYSAYL VAEKVIVTTK HNDDEQYIWE SQAGGSFTVT RDTDGEQLGR GTKITLFLKE DQLEYLEERR IKDLVKKHSE
201: FISYPIYLWT EKTTEKEISD DEDDEPKKEE EGDIEDVDEE KEKDSKKKKK IKEVSHEWQL INKQKPIWLR KPEEITKDEY ASFYKSLTND WEEHLAVKHF
301: SVEGQLEFKA ILFVPKRAPF DLFDTRKKMN NIKLYVRRVF IMDNCEELIP EYLGFVKGVV DSDDLPLNIS REMLQQNKIL KVIRKNLVKK CIEMFNEIAE
401: NKEDYNKFYD AFSKNLKLGI HEDSQNRSKL ADLLRYHSTK SGDEMTSLKD YVTRMKEGQK DIYYITGESK KAVENSPFLE RLKKKGYEVL FMVDAIDEYA
501: VGQLKEYDGK KLVSATKEGL KLDDETEEEK KKKEEKKKSF DELCKVIKDI LGDKVEKVVV SDRIVDSPCC LVTGEYGWSA NMERIMKAQA LRDSSMSGYM
601: SSKKTMEINP DNGIMEELRK RAEADKNDKS VKDLVLLLFE TALLTSGFSL DDPNTFASRI HRMLKLGLSI DEDDNGGDDV DMPPLEEDGA EESKMEEVD

Best Arabidopsis Sequence Match ( AT5G52640.1 )
(BLAST)

001: MADAETFAFQ AEINQLLSLI INTFYSNKEI FLRELISNSS DALDKIRFES LTDKSKLDGQ PELFIRLVPD KSNKTLSIID SGIGMTKADL VNNLGTIARS
101: GTKEFMEALQ AGADVSMIGQ FGVGFYSAYL VAEKVVVTTK HNDDEQYVWE SQAGGSFTVT RDVDGEPLGR GTKITLFLKD DQLEYLEERR LKDLVKKHSE
201: FISYPIYLWT EKTTEKEISD DEDEDEPKKE NEGEVEEVDE EKEKDGKKKK KIKEVSHEWE LINKQKPIWL RKPEEITKEE YAAFYKSLTN DWEDHLAVKH
301: FSVEGQLEFK AILFVPKRAP FDLFDTRKKL NNIKLYVRRV FIMDNCEELI PEYLSFVKGV VDSDDLPLNI SRETLQQNKI LKVIRKNLVK KCIEMFNEIA
401: ENKEDYTKFY EAFSKNLKLG IHEDSQNRGK IADLLRYHST KSGDEMTSFK DYVTRMKEGQ KDIFYITGES KKAVENSPFL ERLKKRGYEV LYMVDAIDEY
501: AVGQLKEYDG KKLVSATKEG LKLEDETEEE KKKREEKKKS FENLCKTIKE ILGDKVEKVV VSDRIVDSPC CLVTGEYGWT ANMERIMKAQ ALRDSSMSGY
601: MSSKKTMEIN PDNGIMEELR KRAEADKNDK SVKDLVMLLY ETALLTSGFS LDEPNTFAAR IHRMLKLGLS IDEDENVEED GDMPELEEDA AEESKMEEVD

Arabidopsis Description

HSP90-1Heat shock protein 90-1 [Source:UniProtKB/Swiss-Prot;Acc:P27323]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.