CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

plastid 2
cytosol 4
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH15953
KRH28827
KRH76626

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G53530.1	KRH15953	AT2G17790.1	17889650
AT5G53530.1	KRH28827	AT2G17790.1	17889650
AT5G53530.1	KRH76626	AT2G17790.1	17889650

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH62711	Soybean	endoplasmic reticulum	98.68	98.68
Solyc04g050240.2.1	Tomato	plastid	90.07	90.37
AT5G53530.1	Thale cress	cytosol	89.07	89.07
Bra003049.1-P	Field mustard	cytosol	88.74	88.74
CDY72159	Canola	cytosol	42.72	87.76
AT4G27690.1	Thale cress	cytosol	86.75	86.47
Bra026315.1-P	Field mustard	cytosol	86.09	85.81
CDY28831	Canola	cytosol	87.09	81.93
CDY19034	Canola	cytosol, endoplasmic reticulum	84.44	81.47
CDY55345	Canola	endoplasmic reticulum	86.75	81.11
KRH18662	Soybean	cytosol	80.13	80.4
GSMUA_Achr5P05770_001	Banana	cytosol	82.78	79.37
Bra022659.1-P	Field mustard	cytosol	87.75	77.26
GSMUA_Achr5P23810_001	Banana	golgi	77.15	72.59
VIT_16s0098g01270.t01	Wine grape	endoplasmic reticulum, vacuole	90.07	72.53
CDY14373	Canola	cytosol, plastid	76.49	66.76
KRG89789	Soybean	plastid	37.09	64.37
CDX96231	Canola	endoplasmic reticulum, extracellular, plasma membrane	87.42	63.46

Protein Annotations

EntrezGene:100813307	Gene3D:2.60.40.640	MapMan:22.5.5.3.3	EMBL:ACUP02004108	InterPro:Arrestin_C	EnsemblPlantsGene:GLYMA_06G312800
GO:GO:0003674	GO:GO:0005215	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005768	GO:GO:0005829	GO:GO:0006810	GO:GO:0006886	GO:GO:0007154	GO:GO:0007165
GO:GO:0008150	GO:GO:0008565	GO:GO:0009987	GO:GO:0016020	GO:GO:0030904	GO:GO:0042147
UniProt:I1KFL5	InterPro:IPR014752	EnsemblPlants:KRH56246	ProteinID:KRH56246	ProteinID:KRH56246.1	PFAM:PF03643
PANTHER:PTHR12233	PANTHER:PTHR12233:SF12	UniParc:UPI000233A8ED	InterPro:Vps26-related	:	:

Description

hypothetical protein

Coordinates

chr6:+:50130531..50136649

Molecular Weight (calculated)

35272.5 Da

IEP (calculated)

7.596

GRAVY (calculated)

-0.432

Length

302 amino acids

Sequence
(BLAST)

001: MNYLLGAFKP ACNVVITFND GKNRKQVPFK KENGQMVTVP LFQSQENIAG KITIEPMQGK KIDHNGVKVE LLGQIEMYFD RGNFYDFTSL VRELDVPGEI
101: YERKTYAFEF STVEMPYETY NGVNVRLRYV LKVTINRGYA GSVVEYQDFV VRNYSPPPQI NNSIKMEVGI EDCLHIEFEY NKSKYHLKDV IIGKIYFLLV
201: RIKIKNMDLE IRRRESTGSG TNTHVETETL AKFELMDGAP VRGESIPIRL FLSPYELTPT HRNINNKFSV KYFLNLVLVD EEDRRYFKQQ EITMYRLQEE
301: TS

Best Arabidopsis Sequence Match ( AT5G53530.1 )
(BLAST)

001: MNYLLGAFKP ACNISITFTD GKNRKQVPTK KDNGQIVMNP LFQSQETIAG KINIEPYQGK KVEHNGVKVE LLGQIEMYFD RGNFYDFTSL VREIDVPGEI
101: YERKTYPFEF SSVEMPYETY NGVNVRLRYV LKVTVTRGYA GSIVEYQDFV VRNYVPLPPI NNSIKMEVGI EDCLHIEFEY NKSKYHLKDV ILGKIYFLLV
201: RIKIKNMDLE IRRRESTGAG ANTHVETETL AKFELMDGAP VRGESIPVRV FLTPYDLTPT HKNINNKFSV KYYLNLVLVD EEDRRYFKQQ EITLYRLKEE
301: TS

Arabidopsis Description

VPS26AVacuolar protein sorting-associated protein 26A [Source:UniProtKB/Swiss-Prot;Acc:Q9FJD0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.