CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus, cytosol

Predictor Summary:

nucleus 2
cytosol 5
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH39040
KRH41871
KRH48661
KRH56246
KRH60559
KRH62711

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G17790.1	KRH56246	AT5G53530.1	17889650
AT2G17790.1	KRH62711	AT5G53530.1	17889650
AT3G51310.1	KRH39040	AT3G17840.1	15618630
AT3G51310.1	KRH41871	AT3G17840.1	15618630
AT3G51310.1	KRH48661	AT3G17840.1	15618630
AT3G51310.1	KRH60559	AT3G17840.1	15618630

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH76626	Soybean	endoplasmic reticulum	98.11	98.11
KRH15953	Soybean	cytosol	7.81	87.32
Solyc02g083560.2.1	Tomato	cytosol	81.99	82.41
PGSC0003DMT400009386	Potato	cytosol	81.74	82.15
PGSC0003DMT400058024	Potato	cytosol	81.74	82.15
Solyc03g033400.2.1	Tomato	cytosol, nucleus	81.49	81.9
VIT_04s0023g03870.t01	Wine grape	cytosol, mitochondrion	85.14	81.06
Zm00001d034492_P006	Maize	endoplasmic reticulum, extracellular	71.41	80.77
TraesCS5A01G448400.1	Wheat	golgi	80.6	80.71
TraesCS5D01G455000.1	Wheat	cytosol	80.48	80.58
EER93269	Sorghum	cytosol	81.49	80.57
Zm00001d013059_P003	Maize	cytosol	81.36	78.4
CDY24377	Canola	cytosol	77.71	78.3
CDY20826	Canola	cytosol	77.71	78.3
Bra002092.1-P	Field mustard	cytosol	77.71	78.3
KRH05971	Soybean	cytosol	78.34	77.94
AT2G17790.1	Thale cress	cytosol	77.2	77.89
KRH17452	Soybean	endoplasmic reticulum	78.09	77.79
Os03t0801600-01	Rice	cytosol	81.36	75.03
CDX73731	Canola	cytosol	72.42	72.78
CDX77995	Canola	cytosol	72.29	72.66
AT3G51310.1	Thale cress	cytosol	72.17	72.53
Bra036818.1-P	Field mustard	cytosol	72.29	72.38
TraesCS5B01G453000.1	Wheat	cytosol	80.48	72.37
CDY59615	Canola	cytosol	71.79	71.52
KRH37553	Soybean	cytosol	8.94	61.21
HORVU7Hr1G081830.7	Barley	cytosol, endoplasmic reticulum, plastid	9.57	42.94

Protein Annotations

Gene3D:1.25.40.660	EntrezGene:100809289	MapMan:22.5.5.3.1	EMBL:ACUP02006766	InterPro:ARM-type_fold	EnsemblPlantsGene:GLYMA_11G079200
GO:GO:0003674	GO:GO:0005215	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005768	GO:GO:0005770	GO:GO:0005829	GO:GO:0006810	GO:GO:0006886
GO:GO:0008150	GO:GO:0008565	GO:GO:0015031	GO:GO:0016020	GO:GO:0030904	GO:GO:0030906
GO:GO:0042147	UniProt:I1LI35	EnsemblPlants:KRH28827	ProteinID:KRH28827	ProteinID:KRH28827.1	PFAM:PF03635
PIRSF:PIRSF009375	PANTHER:PTHR11099	PANTHER:PTHR11099:SF1	SUPFAM:SSF48371	UniParc:UPI000233C23D	InterPro:Vps35
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr11:-:5946944..5955940

Molecular Weight (calculated)

89753.3 Da

IEP (calculated)

5.099

GRAVY (calculated)

-0.196

Length

794 amino acids

Sequence
(BLAST)

001: MMLDGTEDEE KFLAAGIAGL QQNSFYMHRA LDSNNLRDAL KYSAQMLSEL RTSKLSPHKY YELYMRAFDQ LRKLETFFEE ETRRGCSIID LYELVQHAGN
101: ILPRLYLLCT VGSVYIKSKE APAKDVLKDL VEMCRGIQHP VRGLFLRSYL SQVSRDKLPD IGSEYEGDAD TVADAVEFVL QNFTEMNKLW VRMQHQGPAR
201: EKEKREKERS ELRDLVGKNL HVLSQIEGVD LDMYKDVVLP RVLEQVVNCK DELAQFYLMD CIIQVFPDEY HLQTLDVLLG AYPQLQPSVD IKTVLSQLME
301: RLSNYAASSA DVLPEFLQVE AFSKLSNAIG KVIEAQPDMP TVGVVTLYSS LLTFTLHVHP DRLDYADQVL GACVKKLSGK GKIEDNRATK QIVALLSAPL
401: EKYNDIMIAL KLSNYPRVIE YVDIRTTKVM ATVIIQSIMK NGTRISTSEK VEALFELIKG LIKDSDGIPN DELDEDDFKE EQNSVSRLIQ MLYNDDPEEM
501: FKIIDTVRKH ILTGGPKRLP FTVPPLVFSS LKLVRQLQGQ EENPFGDDAS TTPKKIFQLL NQTIETLSGV LAPELALQLY LQCAEAANDC ELEPVAYEFF
601: TQAYILYEEE ISDSRAQITA IHLIIGTLQR MHVFGVENRD TLTHKATGYS AKLLKKPDQC RAVYACSHLF WVDDHDNMKD GERVLLCLKR ALRIANAAQQ
701: MANAARGSTG SVMLFIEILN KYLYFFEKGN LQVTVAAIQG LIELIMNEMQ SDTTTPDPAA NAFLASTMRY IEFQKQKGGA VGEKYEAIKV SHAG

Best Arabidopsis Sequence Match ( AT2G17790.1 )
(BLAST)

001: MIADGSEDEE KWLAAGAAAF KQNAFYMQRA IDSNNLKDAL KYSAQMLSEL RTSKLSPHKY YDLYMRAFDE LRKLEIFFME ETRRGCSVIE LYELVQHAGN
101: ILPRLYLLCT AGSVYIKTKE APAKEILKDL VEMCRGIQHP LRGLFLRSYL AQISRDKLPD IGSEYEGDAD TVIDAVEFVL LNFTEMNKLW VRMQHQGPAR
201: EKERREKERG ELRDLVGKNL HVLSQLEGVD LDMYRDTVLP RVLEQIVNCR DEIAQYYLID CIIQVFPDEY HLQTLDVLLG ACPQLQASVD IMTVLSRLME
301: RLSNYAALNA EVLPYFLQVE AFSKLNNAIG KVIEAQEDMP ILSAVTLYSS LLKFTLHVHP DRLDYADQVL GSCVKQLSGK GKIDDTRATK ELVSLLSAPL
401: EKYNDVVTAL KLTNYPLVVE YLDTETKRIM ATVIVRSIMK NNTLITTAEK VEALFELIKG IINDLDEPQG LEVDEDDFQE EQNSVALLIH MLYNDDPEEM
501: FKIVNVLKKH FLTGGPKRLK FTIPPLVVST LKLIRRLPVE GDNPFGKEAS VTATKIFQFL NQIIEALPNV PSPDLAFRLY LQCAEAADKC DEEPIAYEFF
601: TQAYILYEEE ISDSKAQVTA LQLIIGTLQR MQVFGVENRD TLTHKATGYA AKLLKKPDQC RAVYACSHLF WLEDRETIQD GERVLLCLKR ALKIANSAQQ
701: VANTARGSTG SVTLFIEILN KYLYFYEKGV PQITVESVES LIKLIKNEES MPSDPSAESF FATTLEFMEF QKQKEGAIGE RYQAIKV

Arabidopsis Description

VPS35AVacuolar protein sorting-associated protein 35A [Source:UniProtKB/Swiss-Prot;Acc:Q7X659]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.