Factsheet for Os03t0681700-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 2
cytosol 4
plastid 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os11t0484400-01
Os11t0615250-00
Os12t0446900-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G18140.1	Os11t0484400-01	AT2G16370.1	21798944
AT3G18140.1	Os11t0615250-00	AT2G16370.1	21798944
AT3G18140.1	Os12t0446900-01	AT2G16370.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
HORVU4Hr1G003940.2	Barley	cytosol	94.62	94.62
EES08772	Sorghum	cytosol	94.62	94.62
Zm00001d007577_P001	Maize	extracellular, mitochondrion	94.3	94.3
TraesCS4B01G026200.1	Wheat	cytosol	94.3	94.3
TraesCS4A01G288600.1	Wheat	cytosol	94.3	94.3
TraesCS4D01G023600.1	Wheat	cytosol	93.99	93.99
GSMUA_Achr10P...	Banana	cytosol	90.82	90.82
KRH11050	Soybean	cytosol	86.71	86.71
KRH21226	Soybean	cytosol	86.39	86.39
Solyc03g059310.2.1	Tomato	cytosol	63.61	85.9
VIT_06s0004g04740.t01	Wine grape	cytosol	83.54	83.54
CDX92201	Canola	cytosol	78.48	81.31
CDX95783	Canola	cytosol	78.16	80.98
AT3G18140.1	Thale cress	cytosol	78.16	80.98
Bra022283.1-P	Field mustard	cytosol	62.34	80.74
VIT_08s0007g08080.t01	Wine grape	cytosol, plastid	84.18	75.14
AT2G22040.1	Thale cress	cytosol	65.51	66.13
CDY13785	Canola	cytosol	54.75	66.03
CDY13786	Canola	cytosol	53.8	64.39
CDX76957	Canola	cytosol	63.29	60.98
Bra031226.1-P	Field mustard	cytosol	63.61	60.73

Protein Annotations

Gene3D:2.130.10.10	MapMan:27.7.1.3	EntrezGene:4333729	ProteinID:AAK00964.1	ProteinID:AAP68374.1	ProteinID:ABF98218.1
ProteinID:BAF12821.1	ProteinID:BAS85765.1	ProteinID:EEE59699.1	InterPro:G-protein_beta_WD-40_rep	GO:GO:0003674	GO:GO:0005488
GO:GO:0005515	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0007154	GO:GO:0007165
GO:GO:0008150	GO:GO:0009987	GO:GO:0016043	GO:GO:0031929	GO:GO:0031931	GO:GO:0031932
GO:GO:0032008	GO:GO:0032956	InterPro:IPR001680	InterPro:IPR015943	InterPro:IPR017986	InterPro:MLST8
EnsemblPlantsGene:Os03g0681700	EnsemblPlants:Os03t0681700-01	PFAM:PF00400	PRINTS:PR00320	ScanProsite:PS00678	PFscan:PS50082
PFscan:PS50294	PANTHER:PTHR19842	PANTHER:PTHR19842:SF0	UniProt:Q9AYE4	InterPro:Quinoprotein_ADH-like_supfam	SMART:SM00320
SUPFAM:SSF50998	UniParc:UPI000009EC27	InterPro:WD40/YVTN_repeat-like_dom_sf	InterPro:WD40_repeat	InterPro:WD40_repeat_CS	InterPro:WD40_repeat_dom
RefSeq:XP_015628391.1	RefSeq:XP_015628392.1	:	:	:	:

Description

OsWD40-81, OsLST8, LST8Component of TORC1(Target of rapamycin complex 1) (Os03t0681700-01)

Coordinates

chr3:+:27120054..27123781

Molecular Weight (calculated)

35325.6 Da

IEP (calculated)

6.777

GRAVY (calculated)

-0.247

Length

316 amino acids

Sequence
(BLAST)

001: MAQPSVILAT ASYDHSIKFW EAKSGRCYRT LQHTESHINR LEITPDKRFL AAAGNPHIRL FDINSNSNHP VISYDSHTSN VMAVGFHCDG NWMYSGSEDG
101: TVRIWDLRTA TCQREYESRA AVNTVVLHPN QKELISGDQN GNIRVWDLAA NSCSCELVPE VDTAVRSLTV MWDGSMVVAA NNRGTCYVWR LLKGTQTITC
201: FEPLHKLQAH DGYILKCLLS PEFCDPNRYL ATASSDHTVK IWNVDGFKLE KTLVGHQRWV WDCVFSVDGA YLITASSDTT ARLWTMSTGE AIRVYTSHHK
301: PVVCCALHDG AESAPS

Best Arabidopsis Sequence Match ( AT3G18140.1 )
(BLAST)

001: MSQPSVILAT ASYDHTIRFW EAETGRCYRT IQYPDSHVNR LEITPDKHYL AAACNPHIRL FDVNSNSPQP VMTYDSHTNN VMAVGFQCDA KWMYSGSEDG
101: TVKIWDLRAP GCQKEYESVA AVNTVVLHPN QTELISGDQN GNIRVWDLRA NSCSCELVPE VDTAVRSLTV MWDGTMVVAA NNRGTCYVWR LLRGKQTMTE
201: FEPLHKLQAH NGHILKCLLS PANKYLATAS SDKTVKIWNV DGFKLEKVLT GHQRWVWDCV FSVDGEFLVT ASSDMTARLW SMPAGKEVKV YQGHHKATVC
301: CALHD

Arabidopsis Description

LST8-1 [Source:UniProtKB/TrEMBL;Acc:A0A178VME6]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.