Factsheet for Os03t0703400-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 3
cytosol 2
mitochondrion 1
plastid 3

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0656400-00
Os05t0571200-01
Os06t0158100-00
Os08t0386200-01
Os09t0334500-01
Os10t0533600-01
Os11t0685700-00

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G08480.1	Os10t0533600-01	AT4G01370.1	22643122
AT4G08500.1	Os01t0656400-00	AT4G23810.1	17587183
AT4G08500.1	Os05t0571200-01	AT4G23810.1	17587183
AT4G08500.1	Os06t0158100-00	AT4G23810.1	17587183
AT4G08500.1	Os08t0386200-01	AT4G23810.1	17587183
AT4G08500.1	Os09t0334500-01	AT4G23810.1	17587183
AT4G08500.1	Os11t0685700-00	AT4G23810.1	17587183

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
OQU91136	Sorghum	cytosol	51.07	90.03
Zm00001d033655_P003	Maize	nucleus, plastid	77.98	83.88
Zm00001d013477_P008	Maize	plastid	78.13	83.36
HORVU4Hr1G006660.1	Barley	plastid	79.66	78.7
TraesCS4D01G038900.1	Wheat	plastid	76.61	78.04
TraesCS4A01G272500.1	Wheat	plastid	78.29	77.58
TraesCS4B01G041600.1	Wheat	plastid	78.29	76.88
GSMUA_Achr11P...	Banana	cytosol	55.66	66.79
GSMUA_Achr10P...	Banana	cytosol	53.06	66.1
PGSC0003DMT400067237	Potato	cytosol	28.9	64.95
GSMUA_Achr7P11850_001	Banana	mitochondrion, nucleus	53.21	62.82
GSMUA_Achr1P12210_001	Banana	mitochondrion	55.05	62.28
GSMUA_Achr2P07690_001	Banana	nucleus	54.59	61.55
Bra039693.1-P	Field mustard	cytosol	25.23	60.22
VIT_12s0142g00700.t01	Wine grape	cytosol	23.85	59.09
Os03t0262200-00	Rice	cytosol, plastid	52.29	57.29
CDY36607	Canola	cytosol	27.98	55.62
KRH10459	Soybean	cytosol	37.31	55.45
CDY24072	Canola	cytosol	29.51	54.21
KRH16616	Soybean	nucleus	46.02	51.02
KRH53173	Soybean	nucleus	43.27	50.99
VIT_18s0001g03960.t01	Wine grape	mitochondrion, nucleus	44.19	50.97
KRH64744	Soybean	nucleus	43.88	50.71
VIT_12s0034g00750.t01	Wine grape	cytosol	24.16	50.48
KRH16614	Soybean	mitochondrion	42.35	50.09
KRH18819	Soybean	nucleus	45.41	50.0
VIT_12s0034g00240.t01	Wine grape	nucleus	31.5	48.82
KRH42244	Soybean	cytosol	34.56	48.09
KRH58364	Soybean	cytosol, plastid	35.93	47.0
Bra000647.1-P	Field mustard	cytosol	38.23	46.21
CDY34000	Canola	cytosol	38.53	45.82
CDY36198	Canola	cytosol	40.21	45.74
CDY69517	Canola	cytosol	38.23	45.54
CDY18020	Canola	cytosol	5.35	44.87
Solyc07g053170.2.1	Tomato	cytosol	40.52	44.09
AT4G08500.1	Thale cress	cytosol, mitochondrion	40.83	43.91
Bra037860.1-P	Field mustard	cytosol	40.21	43.91
CDX75154	Canola	cytosol	40.52	43.8
Solyc01g104530.2.1	Tomato	nucleus	42.05	41.35
PGSC0003DMT400061014	Potato	mitochondrion	41.9	41.14
KRH10458	Soybean	cytosol	28.13	38.98
Os02t0769700-01	Rice	mitochondrion	13.0	38.64
AT4G08470.1	Thale cress	cytosol	29.51	34.46
CDY18021	Canola	nucleus	20.8	33.17
AT4G08480.1	Thale cress	cytosol, plastid	37.92	32.08
Os11t0207200-01	Rice	cytosol, plasma membrane	22.48	26.53
Os01t0699400-01	Rice	mitochondrion	13.91	22.09
Os01t0699600-01	Rice	mitochondrion	14.37	21.76
Os02t0555900-00	Rice	plasma membrane	23.85	21.76
Os03t0292700-01	Rice	mitochondrion	14.22	20.95
Os01t0699100-01	Rice	mitochondrion	13.76	20.93
Os04t0437600-01	Rice	plasma membrane	22.63	20.9
Os01t0699500-01	Rice	mitochondrion	13.3	20.57
Os07t0119000-01	Rice	mitochondrion, plastid	22.63	19.65
Os04t0559800-01	Rice	plasma membrane	26.76	19.57
Os03t0764300-01	Rice	mitochondrion, plastid	23.24	19.56
Os05t0545400-01	Rice	mitochondrion, plastid	12.39	19.1
Os05t0545300-01	Rice	cytosol	12.84	19.09
Os02t0666300-01	Rice	plasma membrane	25.69	18.79
Os02t0322400-00	Rice	mitochondrion	12.84	18.75
Os08t0421800-01	Rice	cytosol	18.96	17.97
Os10t0130000-00	Rice	nucleus	14.22	12.38
Os04t0660500-01	Rice	cytosol, plasma membrane	17.74	8.55
Os09t0383300-01	Rice	nucleus	0.92	2.08

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.2.2	Gene3D:3.30.200.20	EntrezGene:4333845	ProteinID:AAS07241.1	ProteinID:ABF98436.1
ProteinID:BAF12931.1	ProteinID:BAS85961.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672
GO:GO:0004674	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0006464	GO:GO:0006468	GO:GO:0006950	GO:GO:0007049	GO:GO:0007154
GO:GO:0007165	GO:GO:0007346	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301
GO:GO:0016310	GO:GO:0016740	GO:GO:0019538	GO:GO:0023014	GO:GO:0031098	GO:GO:0032147
InterPro:IPR000719	InterPro:Kinase-like_dom_sf	EnsemblPlantsGene:Os03g0703400	EnsemblPlants:Os03t0703400-01	PFAM:PF00069	ScanProsite:PS00107
ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24361	PANTHER:PTHR24361:SF404	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS
UniProt:Q75I98	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI00002394F0	RefSeq:XP_015630324.1
SEG:seg	:	:	:	:	:

Description

Similar to MAP3K beta 3 protein kinase (EC 2.7.1.37) (Fragment). (Os03t0703400-01)

Coordinates

chr3:-:28269607..28275709

Molecular Weight (calculated)

70097.2 Da

IEP (calculated)

4.840

GRAVY (calculated)

-0.351

Length

654 amino acids

Sequence
(BLAST)

001: MFSWSRKQSS SSGRRGADAS SMDSSSRGGG GEGSGSGSRG RSSRLERRNA VKHIDYEAAG AGAGAGASSV PACASWSSSM SADRSLGLRP SRSLDLAVGG
101: GGTDIRISGS VEGEVDELCR SLGLSGPEEF AIPVAAWEAR KERSNSDLLP RSRFVSSPPV DDPSPMARTI SAPEVIQCDL PPSFPASIPE ESLNSSSNST
201: ATDSAEEPTA AALGQESPKA APAVAAVAPL AGLPLLSPKR GGGEVGIRGA RPPVLSPPQP LMALAPPPMR RSIVAKDMSG VSAWDIVNSF APSEEKSEVR
301: TDDERVDASH MSDTEEEEEF ADEGVAGVDG ELKELRIGET FEGFTGTSSL STTNDDDASS TTTEAMFIIS PNGKFKRKIK SWMRGALLGS GSFGMVYEGI
401: SDEGAFFAVK EVSLLDQGSN AQQSILALEQ EIALLSQFEH ENIVQYYGTD KEESKLYIFI ELVTQGSLSS LYQKYKLRDS QVSAYTRQIL NGLVYLHERN
501: VVHRDIKCAN ILVHANGSVK LADFGLAKEM SKINMLRSCK GSVYWMAPEV VNPKKTYGPQ ADIWSLGCTV LEMLTRNIPY PNVEWTNAFF MIGKGERPQI
601: PSYLSKDAQD FISQCVQVDP EQRPSASQLM SHPFVNRPLR ASFESASPPA ISSY

Best Arabidopsis Sequence Match ( AT4G08500.1 )
(BLAST)

001: MDRILARMKK STGRRGGDKN ITPVRRLERR DAARNINYDA ASCSSSSAED LSVSTSSLMT RSLEFPEPTS FRIGGGVGEM DRIYRSLGVS GPDDLAISFD
101: AWEACKKRSS SDVVNRFKSF DLDKVRDQDL SEEGPSGVVV GSDSMNHKVQ GQDLSEAGPS GGIVTELSEI GNLITPVDRL VADGVVENRR VMERTPTIVK
201: SKGYLVPNNV VAVGVGVGGG IKGLRPPVLK PPPAMKRPPI DHRGSSWDFL THFAPSETVK RPSSSSSSSE DGCDEEEGKE EEAEAEEMGA RFIQLGDTAD
301: ETCSFTTNEG DSSSTVSNTS PIYPDGGAII TSWQKGQLLG RGSFGSVYEG ISGDGDFFAV KEVSLLDQGS QAQECIQQLE GEIKLLSQLQ HQNIVRYRGT
401: AKDGSNLYIF LELVTQGSLL KLYQRYQLRD SVVSLYTRQI LDGLKYLHDK GFIHRDIKCA NILVDANGAV KLADFGLAKV SKFNDIKSCK GTPFWMAPEV
501: INRKDSDGYG SPADIWSLGC TVLEMCTGQI PYSDLEPVQA LFRIGRGTLP EVPDTLSLDA RLFILKCLKV NPEERPTAAE LLNHPFVRRP LPSVGSGGSG
601: SASPLLRR

Arabidopsis Description

MEKK1Mitogen-activated protein kinase kinase kinase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q39008]
SUBAcon: [mitochondrion,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.