Factsheet for Os06t0153800-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, plastid

Predictor Summary:

nucleus 2
plastid 1
cytosol 2
mitochondrion 1
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704
plastid: 27992503

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0741700-01
Os06t0236300-01
Os08t0420700-01
Os09t0381600-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G26340.1	Os02t0741700-01	AT1G10530.1	21798944
AT3G26340.1	Os06t0236300-01	AT1G10530.1	21798944
AT3G26340.1	Os08t0420700-01	AT1G10530.1	21798944
AT3G26340.1	Os09t0381600-01	AT1G10530.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EER89220	Sorghum	cytosol	81.23	80.36
TraesCS7D01G105500.1	Wheat	cytosol	80.14	79.57
Zm00001d045315_P003	Maize	cytosol	82.67	79.24
TraesCS7B01G008100.1	Wheat	golgi, unclear	79.78	79.21
HORVU5Hr1G110330.1	Barley	cytosol	78.7	78.14
TraesCS7A01G110200.1	Wheat	cytosol	78.34	77.78
KRH54198	Soybean	nucleus	74.01	75.37
VIT_01s0010g02900.t01	Wine grape	cytosol	73.65	75.0
KRH63751	Soybean	cytosol	71.84	73.16
AT3G26340.1	Thale cress	cytosol	70.76	71.79
PGSC0003DMT400038738	Potato	cytosol	28.52	65.29
AT1G13060.3	Thale cress	cytosol	69.68	64.77
Solyc03g063420.1.1	Tomato	mitochondrion	44.04	63.87
HORVU7Hr1G022970.1	Barley	plastid	76.17	62.43
Bra022650.1-P	Field mustard	cytosol	20.58	52.29
CDY70092	Canola	cytosol	15.52	51.81
GSMUA_Achr1P13460_001	Banana	cytosol	18.77	36.62
Os06t0139900-01	Rice	cytosol, plasma membrane, plastid	24.55	27.64
Os02t0770000-01	Rice	cytosol	23.1	26.02
Os05t0187000-01	Rice	plastid	20.94	21.32

Protein Annotations

MapMan:19.2.6.1.2.5	Gene3D:3.60.20.10	EntrezGene:4340166	EMBL:AK104361	EMBL:AK104520	ProteinID:BAD69286.1
ProteinID:BAF18756.1	ProteinID:BAS96217.1	GO:GO:0000502	GO:GO:0003674	GO:GO:0003824	GO:GO:0004175
GO:GO:0004298	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005737
GO:GO:0005839	GO:GO:0006508	GO:GO:0008150	GO:GO:0008152	GO:GO:0008233	GO:GO:0009056
GO:GO:0009987	GO:GO:0016787	GO:GO:0019538	GO:GO:0051603	InterPro:IPR023333	InterPro:IPR029055
InterPro:Ntn_hydrolases_N	EnsemblPlantsGene:Os06g0153800	EnsemblPlants:Os06t0153800-01	PFAM:PF00227	PRINTS:PR00141	ScanProsite:PS00854
PFscan:PS51476	PANTHER:PTHR11599	PANTHER:PTHR11599:SF63	InterPro:Pept_T1A_subB	InterPro:Proteasome_bsu_CS	InterPro:Proteasome_suB-type
InterPro:Proteasome_sua/b	UniProt:Q5VMN4	SUPFAM:SSF56235	MEROPS:T01.A10	UniParc:UPI00004580E7	RefSeq:XP_015643537.1
SEG:seg	:	:	:	:	:

Description

20S proteasome beta5 subunitBeta 5 subunit of 20S proteasome. (Os06t0153800-01)

Coordinates

chr6:-:2771073..2774015

Molecular Weight (calculated)

29786.5 Da

IEP (calculated)

5.651

GRAVY (calculated)

-0.153

Length

277 amino acids

Sequence
(BLAST)

001: MKLDVSAMEN NFAAHAAGGE DDGGLFGAGA DLPAMELPTC PADFDGFQKE TKEMLKHKKG TTTLAFIFDK GVIVAADSRA SMGGYISSQT VRKIIEINPY
101: MLGTMAGGAA DCQFWHRNLG IKCRLHELAN KRRISIAGAS KLLANILYSY RGMGLSIGTM IAGWDEKGPG LYYVDSEGAR LMGSRFSVGS GSLYAYGILD
201: EGYRYVMPVE EAAELARRAI YQATFRDGAS GGCVSVYHVG PNGWTKLSGD DVGELHYKYY PVEATPVEQE MADAPAA

Best Arabidopsis Sequence Match ( AT1G13060.1 )
(BLAST)

001: MKLDTSGFET SMPMIGFGSS SDMLDELSSV PSFDLPRTKE FDGFQKKAKD MLKHAKGTTT LAFIFKGGVM VAADSRASMG GYISSQSVKK IIEINPYMLG
101: TMAGGAADCQ FWHRNLGIKC RLHELANKRR ISVSGASKLL ANMLYSYRGM GLSVGTMIAG WDETGPGLYY VDNEGGRLKG DRFSVGSGSP YAYGVLDSGY
201: KYDMSVEEAS ELARRSIYHA TFRDGASGGV ASVYHVGPEG WTKLSGDDVG ELHYHYYPVA PATAEQVMEE ATAE

Arabidopsis Description

PBE120S proteasome beta subunit E1 [Source:UniProtKB/TrEMBL;Acc:F4HP14]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.