Factsheet for Bra012805.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 2
plastid 1
extracellular 4
endoplasmic reticulum 5
vacuole 4
plasma membrane 6
golgi 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	nucleus, plastid, secretory
BaCelLo:	nucleus
EpiLoc:	plastid
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra018085.1-P
Bra033774.1-P
Bra033775.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G52430.1	Bra018085.1-P	AT3G48090.1	11574472
AT3G52430.1	Bra033774.1-P	AT3G48090.1	11574472
AT3G52430.1	Bra033775.1-P	AT3G48090.1	11574472

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDX90603	Canola	plasma membrane	99.68	99.68
Bra033431.1-P	Field mustard	plastid	50.79	74.42
Bra006922.1-P	Field mustard	cytosol, nucleus, peroxisome	63.97	73.67
AT3G52430.1	Thale cress	cytosol, nucleus, peroxisome	54.92	63.96
VIT_07s0031g02390.t01	Wine grape	cytosol	36.35	37.0
PGSC0003DMT400051170	Potato	cytosol	32.7	35.64
Solyc02g032850.2.1	Tomato	cytosol	32.22	35.12
KRH53943	Soybean	cytosol, nucleus, peroxisome	35.24	35.07
KRH40959	Soybean	cytosol	34.76	34.6
KRH63997	Soybean	cytosol, nucleus, peroxisome	34.6	34.44
KRH59603	Soybean	plastid	27.62	33.27
GSMUA_Achr6P12900_001	Banana	cytosol	31.27	32.67
GSMUA_Achr2P20660_001	Banana	endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	31.11	32.13
Zm00001d004706_P002	Maize	cytosol, peroxisome, plastid	30.79	29.8
EES08158	Sorghum	cytosol, peroxisome, plastid	30.32	28.51
TraesCS4A01G216100.1	Wheat	peroxisome	29.21	28.26
Zm00001d053138_P002	Maize	cytosol, peroxisome, plastid	29.52	28.22
TraesCS4D01G096400.2	Wheat	peroxisome	29.05	28.07
TraesCS4B01G100100.1	Wheat	peroxisome	28.89	27.91
Os11t0195500-01	Rice	mitochondrion	22.86	26.92
HORVU4Hr1G043530.1	Barley	mitochondrion	30.0	26.85
Bra008729.1-P	Field mustard	cytosol	18.41	22.05
Bra006274.1-P	Field mustard	nucleus	16.35	20.56
Bra033775.1-P	Field mustard	cytosol	18.1	19.0
Bra018085.1-P	Field mustard	cytosol	18.1	19.0
Bra033774.1-P	Field mustard	cytosol	17.62	18.75
Bra008731.1-P	Field mustard	extracellular, plasma membrane, vacuole	20.79	18.4

Protein Annotations

MapMan:26.8.2.4.2	Gene3D:3.40.50.1820	InterPro:AB_hydrolase	EnsemblPlantsGene:Bra012805	EnsemblPlants:Bra012805.1	EnsemblPlants:Bra012805.1-P
InterPro:Fungal_lipase-like	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0006629	GO:GO:0008150
GO:GO:0008152	GO:GO:0016020	GO:GO:0016021	GO:GO:0016787	InterPro:IPR029058	UniProt:M4D8J5
PFAM:PF01764	PANTHER:PTHR21493	PANTHER:PTHR21493:SF130	SUPFAM:SSF53474	TMHMM:TMhelix	UniParc:UPI00025407C4
SEG:seg	:	:	:	:	:

Description

AT3G52430 (E=4e-189) PAD4, ATPAD4 | PAD4 (PHYTOALEXIN DEFICIENT 4); lipase/ protein binding / triacylglycerol lipase

Coordinates

chrA03:+:22131168..22136932

Molecular Weight (calculated)

70540.0 Da

IEP (calculated)

4.843

GRAVY (calculated)

-0.304

Length

630 amino acids

Sequence
(BLAST)

001: MGKASGAKHA TVVVAALAFG WLAIEIAFKP FLDKLRSSID KSNPAKDPDD SDKDAAFAAA AAASETTSSV PSLTTQTTQV VMEECQFETS ELQASLMMST
101: PLWSDSWSLC NVADSSKSIQ IQRIAGTMYV ALPEVEINQP GNLVDLKVAG NELFSAFSTS STSDEPPPMV NGAILELFVS SALFIESQIT RGLEKEDREQ
201: VVITGYSTGG TVAALTALWL LSVPLSPSYP LLCITFGSPL LGNQSLSSSI SRSHVAQNFC HVVSIHDLVP RRNDEQLWPF GTYLFCSDNG GLCLDNAASV
301: RRMFHILNST GTPNIEERQR YEHYVSTLSR QFLISRSSLS ENISDNSYDA GVALAVESLG FSDDHPSGVS AKECIETATR ISRAPIERAS ELAIELGDVL
401: PSRLEIQWYK DSCEASPKKL GYYDNFKLYS NPRELKVNMS RAKLAKFWDR VYDMVETNEL PPDFDFELKW LFASQFYQLL AEPLDIAYFY KYKYSRTGTG
501: HYMENGNRPK RYLLFDKWWK ERGECHRVKT ARTRYASTTQ DTCFWAKVEE AKEWLDDVRS EGTNEQRRAS LWEKIVGFEI YANTLVKMKD VSLDVLAENS
601: SYRVWEKKLT EFKKDNGIGM VADESDAMET

Best Arabidopsis Sequence Match ( AT3G52430.1 )
(BLAST)

001: MDDCRFETSE LQASVMISTP LFTDSWSSCN TANCNGSIKI HDIAGITYVA IPAVSMIQLG NLVGLPVTGD VLFPGLSSDE PLPMVDAAIL KLFLQLKIKE
101: GLELELLGKK LVVITGHSTG GALAAFTALW LLSQSSPPSF RVFCITFGSP LLGNQSLSTS ISRSRLAHNF CHVVSIHDLV PRSSNEQFWP FGTYLFCSDK
201: GGVCLDNAGS VRLMFNILNT TATQNTEEHQ RYGHYVFTLS HMFLKSRSFL GGSIPDNSYQ AGVALAVEAL GFSNDDTSGV LVKECIETAT RIVRAPILRS
301: AELANELASV LPARLEIQWY KDRCDASEEQ LGYYDFFKRY SLKRDFKVNM SRIRLAKFWD TVIKMVETNE LPFDFHLGKK WIYASQFYQL LAEPLDIANF
401: YKNRDIKTGG HYLEGNRPKR YEVIDKWQKG VKVPEECVRS RYASTTQDTC FWAKLEQAKE WLDEARKESS DPQRRSLLRE KIVPFESYAN TLVTKKEVSL
501: DVKAKNSSYS VWEANLKEFK CKMGYENEIE MVVDESDAME T

Arabidopsis Description

PAD4Lipase-like PAD4 [Source:UniProtKB/Swiss-Prot;Acc:Q9S745]
SUBAcon: [peroxisome,nucleus,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.