Factsheet for Bra014252.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

extracellular 5
endoplasmic reticulum 6
vacuole 5
plasma membrane 8
golgi 5
cytosol 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, secretory
BaCelLo:	secretory
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	plasma membrane
Plant-mPloc:	endoplasmic reticulum
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra002403.1-P
Bra006619.1-P
Bra012567.1-P
Bra013361.1-P
Bra020182.1-P
Bra030034.1-P
Bra032817.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G50430.1	Bra030034.1-P	AT1G27300.1	21798944
AT1G50430.1	Bra032817.1-P	AT1G27300.1	21798944
AT1G50430.1	Bra012567.1-P	AT4G19030.1	21798944
AT1G50430.1	Bra013361.1-P	AT4G19030.1	21798944
AT1G50430.1	Bra002403.1-P	AT5G22290.1	21798944
AT1G50430.1	Bra006619.1-P	AT5G22290.1	21798944
AT1G50430.1	Bra020182.1-P	AT5G22290.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY45061	Canola	plasma membrane	100.0	100.0
CDY03603	Canola	plasma membrane	99.77	99.77
Bra030492.1-P	Field mustard	extracellular, plasma membrane	30.32	94.93
AT1G50430.1	Thale cress	plasma membrane	94.44	94.44
VIT_09s0054g00930.t01	Wine grape	plasma membrane	87.5	87.1
Solyc01g009310.2.1	Tomato	nucleus, plasma membrane	84.49	84.1
KRH44662	Soybean	endoplasmic reticulum	84.03	84.03
PGSC0003DMT400038174	Potato	mitochondrion, plasma membrane	84.26	83.87
GSMUA_Achr6P30910_001	Banana	plasma membrane	82.64	82.26
EES05015	Sorghum	plasma membrane	84.72	80.62
Os02t0465400-01	Rice	plasma membrane	83.33	80.0
Solyc06g074090.2.1	Tomato	nucleus	78.47	78.11
PGSC0003DMT400015184	Potato	plasma membrane	78.47	78.11
Zm00001d016463_P001	Maize	plastid	82.41	76.72
TraesCS3D01G484700.1	Wheat	golgi, peroxisome, plasma membrane	80.09	75.88
TraesCS3A01G488600.1	Wheat	plasma membrane	79.63	75.77
TraesCS3B01G536500.1	Wheat	plasma membrane	79.17	75.0
Bra041028.1-P	Field mustard	plasma membrane	57.87	73.1
HORVU3Hr1G107040.11	Barley	plastid	80.56	65.17
Bra019638.1-P	Field mustard	plasma membrane	21.76	26.48

Protein Annotations

Gene3D:1.20.120.1630	MapMan:5.5.1.6	EnsemblPlantsGene:Bra014252	EnsemblPlants:Bra014252.1	EnsemblPlants:Bra014252.1-P	InterPro:Ergosterol_biosynth_ERG4_ERG24
GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0008150	GO:GO:0008152	GO:GO:0016020
GO:GO:0016021	GO:GO:0016628	GO:GO:0055114	UniProt:M4DCN6	PFAM:PF01222	ScanProsite:PS01017
ScanProsite:PS01018	PANTHER:PTHR21257	PANTHER:PTHR21257:SF38	InterPro:Sterol_reductase_CS	TMHMM:TMhelix	UniParc:UPI0002545FB5
SEG:seg	:	:	:	:	:

Description

AT1G50430 (E=6e-249) DWF5, PA, LE, ST7R, 7RED | DWF5 (DWARF 5); sterol delta7 reductase

Coordinates

chrA08:+:2171503..2174952

Molecular Weight (calculated)

49342.4 Da

IEP (calculated)

8.668

GRAVY (calculated)

0.337

Length

432 amino acids

Sequence
(BLAST)

001: MAATVHSPIV TYASMLSLLA FCPPFVILLW YTMVHQEGSV VQTGSFLWEN GVQGLINIWP RPTAIAWKII FCYGAFEAAL QLLLPGKRVE GPISPTGNRP
101: VYKANGMAAY FVTLATYLGL WWFGIFNPAI VYDHLGEIFS ALIFGSLVFC VLLYIKGHVA PSSSDSGSCG NLIIDFYWGM ELYPRIGKNF DIKVFTNCRF
201: GMMSWAVLAV TYCIKQYEIN GKVSDSMLVN TILMLVYVTK FFWWEAGYWN TMDIAHDRAG FYICWGCLVW VPSIYTSPGM YLVNHPVQLG TQLAIYILVA
301: GCLCIYINYD CDRQRQEFRR TNGKCSVWGK APSKIVATYT TTAGETKTSL LLTSGWWGLA RHFHYVPEIS SAFFWTVPAL FNNFLPYFYV IFLTILLFDR
401: AKRDDDRCRS KYGKYWKLYC EKVKYRIIPG IY

Best Arabidopsis Sequence Match ( AT1G50430.1 )
(BLAST)

001: MAETVHSPIV TYASMLSLLA FCPPFVILLW YTMVHQDGSV TQTFGFFWEN GVQGLINIWP RPTLIAWKII FCYGAFEAIL QLLLPGKRVE GPISPAGNRP
101: VYKANGLAAY FVTLATYLGL WWFGIFNPAI VYDHLGEIFS ALIFGSFIFC VLLYIKGHVA PSSSDSGSCG NLIIDFYWGM ELYPRIGKSF DIKVFTNCRF
201: GMMSWAVLAV TYCIKQYEIN GKVSDSMLVN TILMLVYVTK FFWWEAGYWN TMDIAHDRAG FYICWGCLVW VPSVYTSPGM YLVNHPVELG TQLAIYILVA
301: GILCIYINYD CDRQRQEFRR TNGKCLVWGR APSKIVASYT TTSGETKTSL LLTSGWWGLA RHFHYVPEIL SAFFWTVPAL FDNFLAYFYV IFLTLLLFDR
401: AKRDDDRCRS KYGKYWKLYC EKVKYRIIPG IY

Arabidopsis Description

DWF57-dehydrocholesterol reductase [Source:UniProtKB/Swiss-Prot;Acc:Q9LDU6]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.