Factsheet for Bra041028.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

extracellular 5
endoplasmic reticulum 6
vacuole 5
plasma membrane 7
golgi 5
cytosol 1
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, plastid, secretory
BaCelLo:	secretory
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	plasma membrane
Plant-mPloc:	endoplasmic reticulum
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	plasma membrane

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra002403.1-P
Bra006619.1-P
Bra012567.1-P
Bra013361.1-P
Bra020182.1-P
Bra030034.1-P
Bra032817.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G50430.1	Bra030034.1-P	AT1G27300.1	21798944
AT1G50430.1	Bra032817.1-P	AT1G27300.1	21798944
AT1G50430.1	Bra012567.1-P	AT4G19030.1	21798944
AT1G50430.1	Bra013361.1-P	AT4G19030.1	21798944
AT1G50430.1	Bra002403.1-P	AT5G22290.1	21798944
AT1G50430.1	Bra006619.1-P	AT5G22290.1	21798944
AT1G50430.1	Bra020182.1-P	AT5G22290.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Bra030492.1-P	Field mustard	extracellular, plasma membrane	28.66	71.01
CDY29319	Canola	plasma membrane	74.56	61.3
Bra014252.1-P	Field mustard	plasma membrane	73.1	57.87
AT1G50430.1	Thale cress	plasma membrane	72.51	57.41
VIT_09s0054g00930.t01	Wine grape	plasma membrane	68.42	53.92
KRH44662	Soybean	endoplasmic reticulum	65.5	51.85
Solyc01g009310.2.1	Tomato	nucleus, plasma membrane	65.5	51.61
GSMUA_Achr6P30910_001	Banana	plasma membrane	65.5	51.61
PGSC0003DMT400038174	Potato	mitochondrion, plasma membrane	65.5	51.61
Os02t0465400-01	Rice	plasma membrane	66.08	50.22
EES05015	Sorghum	plasma membrane	66.08	49.78
TraesCS3A01G488600.1	Wheat	plasma membrane	64.91	48.9
TraesCS3B01G536500.1	Wheat	plasma membrane	64.91	48.68
TraesCS3D01G484700.1	Wheat	golgi, peroxisome, plasma membrane	64.91	48.68
Zm00001d016463_P001	Maize	plastid	64.04	47.2
PGSC0003DMT400015184	Potato	plasma membrane	59.36	46.77
Solyc06g074090.2.1	Tomato	nucleus	59.36	46.77
HORVU3Hr1G107040.11	Barley	plastid	65.5	41.95
Bra019638.1-P	Field mustard	plasma membrane	17.25	16.62

Protein Annotations

EnsemblPlants:Bra041028.1	EnsemblPlants:Bra041028.1-P	EnsemblPlantsGene:Bra041028	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575
GO:GO:0008150	GO:GO:0008152	GO:GO:0016020	GO:GO:0016021	GO:GO:0016628	GO:GO:0055114
InterPro:Ergosterol_biosynth_ERG4_ERG24	InterPro:Sterol_reductase_CS	PANTHER:PTHR21257	PANTHER:PTHR21257:SF38	PFAM:PF01222	ScanProsite:PS01017
TMHMM:TMhelix	UniParc:UPI000253EC1A	UniProt:M4FIU9	MapMan:5.5.1.6	:	:

Description

AT1G50430 (E=5e-131) DWF5, PA, LE, ST7R, 7RED | DWF5 (DWARF 5); sterol delta7 reductase

Coordinates

chrScaffold000395:+:793..3465

Molecular Weight (calculated)

38929.7 Da

IEP (calculated)

8.517

GRAVY (calculated)

0.368

Length

342 amino acids

Sequence
(BLAST)

001: MAETVHSPIV AFALMLSLLA LCPPFVILLW FTRMVLLFRP LASCGRMEFK GSSTYGQDPL PSLGRLSFAT EHLRLLFSFF FLVKELKAQF LQRETDQFTR
101: SDRWLYLSSG IFNPAIVYDH LGAIFSALIF GCFIFCVLLY VKVHVAPSSS DSGSCGNLII DFYWGMELYP RIGKNFDIKV FTNCRFGMMS WAVLAVTYCI
201: KQYEINGRVS DSMLVNTILM HVYVTKFFGW EAGYWNSMDI AHDRAGFYIC WGCLVWVPSI YTSPGMYLVN HPVQLGTQLA IYILVAGILC IYINYDCDRQ
301: RQEFRRTNGK CSVWGKAPSK IVATYKTTAS ETKTSLLLTS GW

Best Arabidopsis Sequence Match ( AT1G50430.2 )
(BLAST)

001: MAETVHSPIV TYASMLSLLA FCPPFVILLW YTMVHQDGSV TQTFGFFWEN GVQGLINIWP RPTLIAWKII FCYGAFEAIL QLLLPGKRVE GPISPAGNRP
101: VYKANGLAAY FVTLATYLGL WFGIFNPAIV YDHLGEIFSA LIFGSFIFCV LLYIKGHVAP SSSDSGSCGN LIIDFYWGME LYPRIGKSFD IKVFTNCRFG
201: MMSWAVLAVT YCIKQYEING KVSDSMLVNT ILMLVYVTKF FWWEAGYWNT MDIAHDRAGF YICWGCLVWV PSVYTSPGMY LVNHPVELGT QLAIYILVAG
301: ILCIYINYDC DRQRQEFRRT NGKCLVWGRA PSKIVASYTT TSGETKTSLL LTSGWWGLAR HFHYVPEILS AFFWTVPALF DNFLAYFYVI FLTLLLFDRA
401: KRDDDRCRSK YGKYWKLYCE KVKYRIIPGI Y

Arabidopsis Description

DWF57-dehydrocholesterol reductase [Source:UniProtKB/Swiss-Prot;Acc:Q9LDU6]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.