Factsheet for Bra030492.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, extracellular

Predictor Summary:

extracellular 5
endoplasmic reticulum 5
vacuole 4
plasma membrane 5
golgi 4
cytosol 1
mitochondrion 1
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	extracellular, plasma membrane
Any Predictor:	cytosol, mitochondrion, plastid, secretory
BaCelLo:	secretory
EpiLoc:	cytosol
iPSORT:	mitochondrion
MultiLoc:	plasma membrane
Plant-mPloc:	endoplasmic reticulum
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	extracellular

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra002403.1-P
Bra006619.1-P
Bra012567.1-P
Bra013361.1-P
Bra020182.1-P
Bra030034.1-P
Bra032817.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G50430.1	Bra030034.1-P	AT1G27300.1	21798944
AT1G50430.1	Bra032817.1-P	AT1G27300.1	21798944
AT1G50430.1	Bra012567.1-P	AT4G19030.1	21798944
AT1G50430.1	Bra013361.1-P	AT4G19030.1	21798944
AT1G50430.1	Bra002403.1-P	AT5G22290.1	21798944
AT1G50430.1	Bra006619.1-P	AT5G22290.1	21798944
AT1G50430.1	Bra020182.1-P	AT5G22290.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Bra014252.1-P	Field mustard	plasma membrane	94.93	30.32
AT1G50430.1	Thale cress	plasma membrane	92.03	29.4
Bra041028.1-P	Field mustard	plasma membrane	71.01	28.66
VIT_09s0054g00930.t01	Wine grape	plasma membrane	86.96	27.65
GSMUA_Achr6P30910_001	Banana	plasma membrane	84.06	26.73
KRH44662	Soybean	endoplasmic reticulum	83.33	26.62
PGSC0003DMT400038174	Potato	mitochondrion, plasma membrane	83.33	26.5
CDY07287	Canola	endoplasmic reticulum, plasma membrane, vacuole	27.54	26.39
Solyc01g009310.2.1	Tomato	nucleus, plasma membrane	82.61	26.27
Os02t0465400-01	Rice	plasma membrane	85.51	26.22
EES05015	Sorghum	plasma membrane	84.78	25.77
Solyc06g074090.2.1	Tomato	nucleus	79.71	25.35
TraesCS3A01G488600.1	Wheat	plasma membrane	83.33	25.33
TraesCS3B01G536500.1	Wheat	plasma membrane	83.33	25.22
TraesCS3D01G484700.1	Wheat	golgi, peroxisome, plasma membrane	83.33	25.22
PGSC0003DMT400015184	Potato	plasma membrane	78.99	25.12
Zm00001d016463_P001	Maize	plastid	83.33	24.78
HORVU3Hr1G107040.11	Barley	plastid	83.33	21.54
CDY07286	Canola	endoplasmic reticulum, plasma membrane, vacuole	34.06	15.21
Bra019638.1-P	Field mustard	plasma membrane	19.57	7.61

Protein Annotations

EnsemblPlants:Bra030492.1	EnsemblPlants:Bra030492.1-P	EnsemblPlantsGene:Bra030492	GO:GO:0005575	GO:GO:0016020	GO:GO:0016021
InterPro:Ergosterol_biosynth_ERG4_ERG24	PANTHER:PTHR21257	PANTHER:PTHR21257:SF38	PFAM:PF01222	TMHMM:TMhelix	UniParc:UPI0002547BC1
UniProt:M4ENX2	MapMan:50.1.3	:	:	:	:

Description

AT1G50430 (E=9e-037) DWF5, PA, LE, ST7R, 7RED | DWF5 (DWARF 5); sterol delta7 reductase

Coordinates

chrA05:+:11863721..11865573

Molecular Weight (calculated)

15791.4 Da

IEP (calculated)

8.841

GRAVY (calculated)

0.162

Length

138 amino acids

Sequence
(BLAST)

001: MAWKIIFCYR AFEAALQLLL PGKRVEGPVS PTGNRPVYKV HVAPSSSDSG SCRNLIIDFY WGMELYPRIA GFYICWGCLV WVPSIYTSPG MYLVNHPVQL
101: GTQLAIYILV AGILCIYINY DCDRQRQEFR RTNRKCSV

Best Arabidopsis Sequence Match ( AT1G50430.2 )
(BLAST)

001: MAETVHSPIV TYASMLSLLA FCPPFVILLW YTMVHQDGSV TQTFGFFWEN GVQGLINIWP RPTLIAWKII FCYGAFEAIL QLLLPGKRVE GPISPAGNRP
101: VYKANGLAAY FVTLATYLGL WFGIFNPAIV YDHLGEIFSA LIFGSFIFCV LLYIKGHVAP SSSDSGSCGN LIIDFYWGME LYPRIGKSFD IKVFTNCRFG
201: MMSWAVLAVT YCIKQYEING KVSDSMLVNT ILMLVYVTKF FWWEAGYWNT MDIAHDRAGF YICWGCLVWV PSVYTSPGMY LVNHPVELGT QLAIYILVAG
301: ILCIYINYDC DRQRQEFRRT NGKCLVWGRA PSKIVASYTT TSGETKTSLL LTSGWWGLAR HFHYVPEILS AFFWTVPALF DNFLAYFYVI FLTLLLFDRA
401: KRDDDRCRSK YGKYWKLYCE KVKYRIIPGI Y

Arabidopsis Description

DWF57-dehydrocholesterol reductase [Source:UniProtKB/Swiss-Prot;Acc:Q9LDU6]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.