CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

plastid 2
cytosol 1
mitochondrion 1
golgi 4
endoplasmic reticulum 4
extracellular 3
plasma membrane 5
vacuole 3

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, mitochondrion, plastid, secretory
BaCelLo:	plastid
ChloroP:	plastid
EpiLoc:	cytosol
iPSORT:	mitochondrion
MultiLoc:	golgi
Plant-mPloc:	endoplasmic reticulum
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

PPI

Inferred distinct locusB in Crop

locusB	locations
EES05123

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G50430.1	EES05123	AT1G27300.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Os02t0465400-01	Rice	plasma membrane	91.63	92.44
Zm00001d016463_P001	Maize	plastid	92.95	90.95
TraesCS3A01G488600.1	Wheat	plasma membrane	86.56	86.56
TraesCS3D01G484700.1	Wheat	golgi, peroxisome, plasma membrane	86.34	85.96
GSMUA_Achr6P30910_001	Banana	plasma membrane	82.16	85.94
TraesCS3B01G536500.1	Wheat	plasma membrane	86.12	85.75
CDY03603	Canola	plasma membrane	80.84	84.95
Bra030492.1-P	Field mustard	extracellular, plasma membrane	25.77	84.78
Bra014252.1-P	Field mustard	plasma membrane	80.62	84.72
CDY45061	Canola	plasma membrane	80.62	84.72
AT1G50430.1	Thale cress	plasma membrane	79.96	84.03
VIT_09s0054g00930.t01	Wine grape	plasma membrane	79.74	83.41
KRH44662	Soybean	endoplasmic reticulum	78.85	82.87
PGSC0003DMT400038174	Potato	mitochondrion, plasma membrane	77.31	80.88
Solyc01g009310.2.1	Tomato	nucleus, plasma membrane	77.31	80.88
PGSC0003DMT400015184	Potato	plasma membrane	73.13	76.5
Solyc06g074090.2.1	Tomato	nucleus	73.13	76.5
HORVU3Hr1G107040.11	Barley	plastid	87.67	74.53
CDY29319	Canola	plasma membrane	63.66	69.47
Bra041028.1-P	Field mustard	plasma membrane	49.78	66.08
CDY07287	Canola	endoplasmic reticulum, plasma membrane, vacuole	16.96	53.47
CDY07286	Canola	endoplasmic reticulum, plasma membrane, vacuole	26.65	39.16
EER96347	Sorghum	plasma membrane	21.81	26.76

Protein Annotations

Gene3D:1.20.120.1630	MapMan:5.5.1.6	EntrezGene:8084478	UniProt:C5Y133	EnsemblPlants:EES05015	ProteinID:EES05015
ProteinID:EES05015.1	InterPro:Ergosterol_biosynth_ERG4_ERG24	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005783	GO:GO:0005794	GO:GO:0005886	GO:GO:0006629
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009918	GO:GO:0016020	GO:GO:0016021
GO:GO:0016126	GO:GO:0016132	GO:GO:0016628	GO:GO:0030176	GO:GO:0055114	PFAM:PF01222
ScanProsite:PS01017	ScanProsite:PS01018	PANTHER:PTHR21257	PANTHER:PTHR21257:SF38	EnsemblPlantsGene:SORBI_3004G140300	unigene:Sbi.7898
InterPro:Sterol_reductase_CS	TMHMM:TMhelix	UniParc:UPI0001A85494	RefSeq:XP_002452039.1	SEG:seg	:

Description

hypothetical protein

Coordinates

chr4:+:40799384..40804789

Molecular Weight (calculated)

51323.8 Da

IEP (calculated)

8.968

GRAVY (calculated)

0.313

Length

454 amino acids

Sequence
(BLAST)

001: MAKPKPSSAG AKSAAAAAAP APPATVHSAL VTYTSMLALL SLCPPFVILL WYTMVHADGS VVRTYEHLRE HGVLEGLKAI WPMPTLVAWK IIFGFGLFEA
101: VLQLLLPGKR FEGPISPAGN VPVYKANGLQ AYAVTLITYL GLWWFGIFNP AIVYDHLGEI YSALVFGSFV FCILLYIKGH VAPSSSDSGS SGNVIIDFYW
201: GMELYPRIGK YFDIKVFTNC RFGMMSWAVL AVTYCIKQYE MNGRVADSML VNTALMLIYI TKFFWWESGY WCTMDIAHDR AGFYICWGCL VWVPSIYTSP
301: GMYLVNHPVN LGPQLALSIL LAGMLCIYIN YDCDRQRQEF RRTNGKCSVW GKAPSKIVAS YQTTKGETKT SLLLTSGWWG LSRHFHYVPE ILSAFFWTVP
401: ALFNHFLPYF YVIFLTILLF DRAKRDDDRC SSKYGKYWKT YCNKVPYRVI PGIY

Best Arabidopsis Sequence Match ( AT1G50430.1 )
(BLAST)

001: MAETVHSPIV TYASMLSLLA FCPPFVILLW YTMVHQDGSV TQTFGFFWEN GVQGLINIWP RPTLIAWKII FCYGAFEAIL QLLLPGKRVE GPISPAGNRP
101: VYKANGLAAY FVTLATYLGL WWFGIFNPAI VYDHLGEIFS ALIFGSFIFC VLLYIKGHVA PSSSDSGSCG NLIIDFYWGM ELYPRIGKSF DIKVFTNCRF
201: GMMSWAVLAV TYCIKQYEIN GKVSDSMLVN TILMLVYVTK FFWWEAGYWN TMDIAHDRAG FYICWGCLVW VPSVYTSPGM YLVNHPVELG TQLAIYILVA
301: GILCIYINYD CDRQRQEFRR TNGKCLVWGR APSKIVASYT TTSGETKTSL LLTSGWWGLA RHFHYVPEIL SAFFWTVPAL FDNFLAYFYV IFLTLLLFDR
401: AKRDDDRCRS KYGKYWKLYC EKVKYRIIPG IY

Arabidopsis Description

DWF57-dehydrocholesterol reductase [Source:UniProtKB/Swiss-Prot;Acc:Q9LDU6]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.