Factsheet for Os02t0465400-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

plastid 2
cytosol 1
mitochondrion 1
golgi 2
endoplasmic reticulum 2
extracellular 1
vacuole 1
plasma membrane 3

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, mitochondrion, plastid, secretory
BaCelLo:	plastid
EpiLoc:	cytosol
iPSORT:	mitochondrion
MultiLoc:	golgi
Plant-mPloc:	endoplasmic reticulum
PProwler:	secretory
TargetP:	plastid
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

plasma membrane: 18260611

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0509600-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G50430.1	Os02t0509600-01	AT1G27300.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES05015	Sorghum	plasma membrane	92.44	91.63
Zm00001d016463_P001	Maize	plastid	90.0	87.28
Bra030492.1-P	Field mustard	extracellular, plasma membrane	26.22	85.51
GSMUA_Achr6P30910_001	Banana	plasma membrane	82.44	85.48
TraesCS3A01G488600.1	Wheat	plasma membrane	86.22	85.46
TraesCS3D01G484700.1	Wheat	golgi, peroxisome, plasma membrane	86.22	85.09
TraesCS3B01G536500.1	Wheat	plasma membrane	86.0	84.87
VIT_09s0054g00930.t01	Wine grape	plasma membrane	80.89	83.87
KRH44662	Soybean	endoplasmic reticulum	80.22	83.56
CDY03603	Canola	plasma membrane	80.22	83.56
CDY45061	Canola	plasma membrane	80.0	83.33
Bra014252.1-P	Field mustard	plasma membrane	80.0	83.33
AT1G50430.1	Thale cress	plasma membrane	79.56	82.87
PGSC0003DMT400038174	Potato	mitochondrion, plasma membrane	77.56	80.41
Solyc01g009310.2.1	Tomato	nucleus, plasma membrane	77.56	80.41
PGSC0003DMT400015184	Potato	plasma membrane	73.33	76.04
Solyc06g074090.2.1	Tomato	nucleus	73.11	75.81
HORVU3Hr1G107040.11	Barley	plastid	87.56	73.78
CDY29319	Canola	plasma membrane	64.0	69.23
Bra041028.1-P	Field mustard	plasma membrane	50.22	66.08
CDY07287	Canola	endoplasmic reticulum, plasma membrane, vacuole	17.78	55.56
CDY07286	Canola	endoplasmic reticulum, plasma membrane, vacuole	27.33	39.81
Os09t0565600-01	Rice	plasma membrane	22.44	27.37
Os01t0354200-01	Rice	endoplasmic reticulum, plasma membrane	19.56	27.33

Protein Annotations

Gene3D:1.20.120.1630	EntrezGene:4329318	MapMan:5.5.1.6	ProteinID:BAD19845.1	ProteinID:BAF08723.1	ProteinID:BAS78582.1
InterPro:Ergosterol_biosynth_ERG4_ERG24	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005783	GO:GO:0005794	GO:GO:0005886	GO:GO:0006629	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009918	GO:GO:0016020	GO:GO:0016021	GO:GO:0016126
GO:GO:0016132	GO:GO:0016628	GO:GO:0030176	GO:GO:0055114	EnsemblPlantsGene:Os02g0465400	EnsemblPlants:Os02t0465400-01
PFAM:PF01222	ScanProsite:PS01017	ScanProsite:PS01018	PANTHER:PTHR21257	PANTHER:PTHR21257:SF38	UniProt:Q6K4V9
InterPro:Sterol_reductase_CS	TMHMM:TMhelix	UniParc:UPI0000375999	RefSeq:XP_015627380.1	RefSeq:XP_015627381.1	SEG:seg

Description

Similar to 7-dehydrocholesterol reductase (EC 1.3.1.21) (7-DHC reductase) (Sterol delta-7-reductase) (Dwarf5 protein). (Os02t0465400-01)

Coordinates

chr2:-:15641302..15647376

Molecular Weight (calculated)

51023.4 Da

IEP (calculated)

8.822

GRAVY (calculated)

0.294

Length

450 amino acids

Sequence
(BLAST)

001: MAKPRASAAA AKAPASTPPK TVHSALVTYA SMLSLLSLCP PFVILLWYTM VHADGSVVRA YEHLREHGVL EGLKAIWPMP TMAAWKIIFG FGLFEAALQL
101: LLPGKRFEGP VSPSGNVPVY KANGLQAYAV TLITYLSLWW FGIFNPAIVY DHLGEIYSAL VFGSFVFCIF LYIKGHLAPS SSDSGSSGNV IIDFYWGMEL
201: YPRIGKHFDI KVFTNCRFGM MSWAVLAVTY CIKQYEMNGR VADSMLVNTA LMLIYVTKFF WWESGYWCTM DIAHDRAGFY ICWGCLVWVP SIYTSPGMYL
301: VNHPVNLGPQ LALSILLAGI LCIYINYDCD RQRQEFRRTN GKCSIWGKAP SKIVASYQTT NGETKSSLLL TSGWWGLSRH FHYVPEILSA FFWTVPALFD
401: HFLPYFYVIF LTILLFDRAK RDDDRCSSKY GKYWKMYCNK VPCRVIPGIY

Best Arabidopsis Sequence Match ( AT1G50430.1 )
(BLAST)

001: MAETVHSPIV TYASMLSLLA FCPPFVILLW YTMVHQDGSV TQTFGFFWEN GVQGLINIWP RPTLIAWKII FCYGAFEAIL QLLLPGKRVE GPISPAGNRP
101: VYKANGLAAY FVTLATYLGL WWFGIFNPAI VYDHLGEIFS ALIFGSFIFC VLLYIKGHVA PSSSDSGSCG NLIIDFYWGM ELYPRIGKSF DIKVFTNCRF
201: GMMSWAVLAV TYCIKQYEIN GKVSDSMLVN TILMLVYVTK FFWWEAGYWN TMDIAHDRAG FYICWGCLVW VPSVYTSPGM YLVNHPVELG TQLAIYILVA
301: GILCIYINYD CDRQRQEFRR TNGKCLVWGR APSKIVASYT TTSGETKTSL LLTSGWWGLA RHFHYVPEIL SAFFWTVPAL FDNFLAYFYV IFLTLLLFDR
401: AKRDDDRCRS KYGKYWKLYC EKVKYRIIPG IY

Arabidopsis Description

DWF57-dehydrocholesterol reductase [Source:UniProtKB/Swiss-Prot;Acc:Q9LDU6]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.