Factsheet for PGSC0003DMT400038174

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion, plasma membrane

Predictor Summary:

nucleus 1
cytosol 1
extracellular 2
endoplasmic reticulum 3
vacuole 2
plasma membrane 5
golgi 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	mitochondrion, plasma membrane
Any Predictor:	cytosol, nucleus, secretory
BaCelLo:	nucleus
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	plasma membrane
Plant-mPloc:	endoplasmic reticulum
PProwler:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

mitochondrion: 24351685

msms PMID: 24351685 doi

F Salvato, JF Havelund, M Chen, RS Rao, A Rogowska-Wrzesinska, ON Jensen, DR Gang, JJ Thelen, IM Møller

Department of Biochemistry and Interdisciplinary Plant Group, University of Missouri, Columbia, Missouri 65211.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400015077
PGSC0003DMT400050482
PGSC0003DMT400071510
PGSC0003DMT400073246
PGSC0003DMT400084737

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G50430.1	PGSC0003DMT400084737	AT1G27300.1	21798944
AT1G50430.1	PGSC0003DMT400071510	AT4G19030.1	21798944
AT1G50430.1	PGSC0003DMT400073246	AT4G19030.1	21798944
AT1G50430.1	PGSC0003DMT400015077	AT5G22290.1	21798944
AT1G50430.1	PGSC0003DMT400050482	AT5G22290.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc01g009310.2.1	Tomato	nucleus, plasma membrane	99.31	99.31
VIT_09s0054g00930.t01	Wine grape	plasma membrane	84.56	84.56
CDY03603	Canola	plasma membrane	84.1	84.49
Bra014252.1-P	Field mustard	plasma membrane	83.87	84.26
CDY45061	Canola	plasma membrane	83.87	84.26
PGSC0003DMT400015184	Potato	plasma membrane	83.87	83.87
Bra030492.1-P	Field mustard	extracellular, plasma membrane	26.5	83.33
AT1G50430.1	Thale cress	plasma membrane	82.03	82.41
KRH44662	Soybean	endoplasmic reticulum	81.8	82.18
GSMUA_Achr6P30910_001	Banana	plasma membrane	80.65	80.65
Os02t0465400-01	Rice	plasma membrane	80.41	77.56
EES05015	Sorghum	plasma membrane	80.88	77.31
TraesCS3A01G488600.1	Wheat	plasma membrane	79.26	75.77
TraesCS3D01G484700.1	Wheat	golgi, peroxisome, plasma membrane	79.26	75.44
TraesCS3B01G536500.1	Wheat	plasma membrane	79.03	75.22
Zm00001d016463_P001	Maize	plastid	79.49	74.35
CDY29319	Canola	plasma membrane	65.21	68.03
HORVU3Hr1G107040.11	Barley	plastid	80.65	65.54
Bra041028.1-P	Field mustard	plasma membrane	51.61	65.5
CDY07287	Canola	endoplasmic reticulum, plasma membrane, vacuole	18.43	55.56
CDY07286	Canola	endoplasmic reticulum, plasma membrane, vacuole	28.11	39.48
PGSC0003DMT400007043	Potato	endoplasmic reticulum	23.5	27.64

Protein Annotations

Gene3D:1.20.120.1630	EntrezGene:102589505	MapMan:5.5.1.6	InterPro:Ergosterol_biosynth_ERG4_ERG24	GO:GO:0003674	GO:GO:0003824
GO:GO:0005575	GO:GO:0008150	GO:GO:0008152	GO:GO:0016020	GO:GO:0016021	GO:GO:0016628
GO:GO:0055114	UniProt:M1B6G0	PFAM:PF01222	EnsemblPlantsGene:PGSC0003DMG400014722	PGSC:PGSC0003DMG400014722	EnsemblPlants:PGSC0003DMT400038174
ScanProsite:PS01018	PANTHER:PTHR21257	PANTHER:PTHR21257:SF38	InterPro:Sterol_reductase_CS	TMHMM:TMhelix	UniParc:UPI000296FA30
RefSeq:XP_006362628.1	SEG:seg	:	:	:	:

Description

Sterol delta-7 reductase DWF5 [Source:PGSC_GENE;Acc:PGSC0003DMG400014722]

Coordinates

chr1:+:6585418..6596019

Molecular Weight (calculated)

49654.0 Da

IEP (calculated)

8.750

GRAVY (calculated)

0.299

Length

434 amino acids

Sequence
(BLAST)

001: MVENKLVHSP LITYGSMLSL LSFTPPFVIL MWYTNEHADG SILKTFNHLR ENGLQGLIDI WPKPTAIAGK LIICYALFEA ALQLLLPGKT VEGPISPTGH
101: RPVYKANGMA AYAVTLITYI SLWWFGIFNP AIVYDHLGEI LSTLIFGSLV FCVLLYIKGH VAPSSTDSGS SGNIIVDFYW GMELYPRIGK HFDIKVFTNC
201: RFGMMSWAVL AVTYCIKQHE EYGRVSDSML VNTILMLVYV TKFFWWEAGY WNTMDIAHDR AGFYICWGCL VWVPSIYTSP GMYLVKQPVN LGLQLALYIL
301: VAGLLCIYIN YDCDRQRQEF RRTNGKCTVW GKTPSKIVAA YTTTSGEKKT SLLLTSGWWG LARHFHYVPE ILAAFFWSVP ALFNHFIPYF YVIFLIILLL
401: DRAKRDDDRC KAKYGKYWKL YCEKVPYRVI PGIY

Best Arabidopsis Sequence Match ( AT1G50430.1 )
(BLAST)

001: MAETVHSPIV TYASMLSLLA FCPPFVILLW YTMVHQDGSV TQTFGFFWEN GVQGLINIWP RPTLIAWKII FCYGAFEAIL QLLLPGKRVE GPISPAGNRP
101: VYKANGLAAY FVTLATYLGL WWFGIFNPAI VYDHLGEIFS ALIFGSFIFC VLLYIKGHVA PSSSDSGSCG NLIIDFYWGM ELYPRIGKSF DIKVFTNCRF
201: GMMSWAVLAV TYCIKQYEIN GKVSDSMLVN TILMLVYVTK FFWWEAGYWN TMDIAHDRAG FYICWGCLVW VPSVYTSPGM YLVNHPVELG TQLAIYILVA
301: GILCIYINYD CDRQRQEFRR TNGKCLVWGR APSKIVASYT TTSGETKTSL LLTSGWWGLA RHFHYVPEIL SAFFWTVPAL FDNFLAYFYV IFLTLLLFDR
401: AKRDDDRCRS KYGKYWKLYC EKVKYRIIPG IY

Arabidopsis Description

DWF57-dehydrocholesterol reductase [Source:UniProtKB/Swiss-Prot;Acc:Q9LDU6]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.