CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 3
mitochondrion 4
nucleus 1
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH25071
KRH43086
KRH59212
KRH59213

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G65030.1	KRH25071	AT4G05420.1	18223036
AT1G65030.1	KRH43086	AT4G05420.1	18223036
AT1G65030.1	KRH59212	AT4G05420.1	18223036
AT1G65030.1	KRH59213	AT4G05420.1	18223036

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH37745	Soybean	nucleus	87.32	94.98
VIT_08s0007g00490.t01	Wine grape	mitochondrion, plastid	60.52	62.13
AT1G65030.1	Thale cress	cytosol, nucleus, plastid	58.5	58.84
CDY12650	Canola	cytosol	58.21	58.72
CDY64887	Canola	cytosol	57.64	58.48
Bra004232.1-P	Field mustard	cytosol	57.35	57.85
CDX96086	Canola	cytosol	57.35	57.85
CDY01817	Canola	cytosol	57.35	57.85
Solyc02g078830.2.1	Tomato	nucleus	55.62	57.27
PGSC0003DMT400057752	Potato	cytosol	55.33	56.97
TraesCS5B01G033700.1	Wheat	endoplasmic reticulum, extracellular, golgi, plasma membrane, plastid, vacuole	49.86	53.73
TraesCS5D01G041900.1	Wheat	endoplasmic reticulum, extracellular, golgi, plasma membrane, plastid, vacuole	49.57	53.58
TraesCS5A01G033600.1	Wheat	mitochondrion, plastid	49.57	53.25
Os12t0615900-01	Rice	nucleus	36.6	50.8
GSMUA_Achr4P31530_001	Banana	cytosol	50.72	50.14
EES16386	Sorghum	plastid	47.26	49.7
HORVU5Hr1G008820.2	Barley	mitochondrion	47.84	47.7
CDY01885	Canola	plastid	21.61	47.17
Zm00001d022257_P002	Maize	plastid	46.4	46.8
Bra033995.1-P	Field mustard	cytosol	46.69	41.97
HORVU0Hr1G027960.1	Barley	plastid	33.43	34.94

Protein Annotations

EntrezGene:100777673	Gene3D:2.130.10.10	MapMan:35.2	EMBL:ACUP02009394	InterPro:G-protein_beta_WD-40_rep	EnsemblPlantsGene:GLYMA_15G195000
GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	UniProt:I1MHU7	InterPro:IPR001680	InterPro:IPR015943
InterPro:IPR017986	EnsemblPlants:KRH12797	ProteinID:KRH12797	ProteinID:KRH12797.1	PFAM:PF00400	PRINTS:PR00320
ScanProsite:PS00678	PFscan:PS50082	PFscan:PS50294	PANTHER:PTHR44675	SMART:SM00320	SUPFAM:SSF50978
UniParc:UPI00023D276F	InterPro:WD40/YVTN_repeat-like_dom_sf	InterPro:WD40_repeat	InterPro:WD40_repeat_CS	InterPro:WD40_repeat_dom	InterPro:WD40_repeat_dom_sf
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr15:+:22258410..22268297

Molecular Weight (calculated)

37505.6 Da

IEP (calculated)

8.288

GRAVY (calculated)

-0.189

Length

347 amino acids

Sequence
(BLAST)

001: MSLVAGSYEK FIWGFTLRSN SQTLNPLFSY PSHLSSIKSV AVSGPVVASG GEDDTIQLYD LAAAASLGSL HTHSATVTAL SFYAPPHLPF PRNLVSADAS
101: GNLSIFDADG FVHLTTLSVH RNSAINDLAL HPSGERALTV GRDECLAVVN LVRGRRNCCL RLGKEASLVK FDASGDQFFM AVKEKVSVHQ TEDARILFEL
201: ECPKPVLCAT PAKNGLLYTG GEDRNITAWD IKTGKIAYCL EEAHTARVKG IVALTESDGA TGDDDPYLVA SASSDGIIRV WDVRMAAREK PLTEHNTRSR
301: LTCLAGSSLK FKRQRPQDGK SKSKVVKQQR ALSGKSKSKV EDQTIEV

Best Arabidopsis Sequence Match ( AT1G65030.1 )
(BLAST)

001: MSIIAGSYER FIWGFKLKPT KHDAESQTLT LSPLFSYPSH ISPITTVACS GPAAASGGSD DTIHLYDLPS ASSLGSLLDH NHAASITALS FYTPSSLSFP
101: RNLISAAADG SVAIFDTDPF VLLKSFRPHK KAVNDLAIHP SGKLALAVYR DEFFAMLNLV RGKRSFCCRL GHEASLVKFD PSGERFYMVV SNKVGVHQSE
201: DAKLLLELEN PSRKRILCAT PGESGTLFTG GEDRAITAWD TNSGKLAYSI EDAHPARIKG IVVLTRNDSD GSLEDPYLIG SASSDGIIRV WDVRMAAKEN
301: TKPLAETNTK SRLTCLAGSA LKSMRRPQIG KQEPQKVDEE HEDSE

Arabidopsis Description

At1g65030 [Source:UniProtKB/TrEMBL;Acc:Q5XEV4]
SUBAcon: [plastid,nucleus,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.