CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 3
cytosol 2
mitochondrion 1
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH13399
KRH20755
KRH62918

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G60940.1	KRH13399	AT5G13810.1	21798944
AT1G60940.1	KRH20755	AT5G13810.1	21798944
AT1G60940.1	KRH62918	AT5G13810.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH44077	Soybean	endoplasmic reticulum, nucleus	96.1	98.01
VIT_12s0035g00310.t01	Wine grape	cytosol	89.69	90.45
PGSC0003DMT400061170	Potato	cytosol	88.02	87.78
Solyc01g103940.2.1	Tomato	plastid	88.02	87.53
Bra031475.1-P	Field mustard	cytosol	85.52	87.22
CDY46190	Canola	cytosol	85.52	87.22
KRH59681	Soybean	endoplasmic reticulum	83.84	85.75
KRH41015	Soybean	endoplasmic reticulum	83.84	85.75
KRH54011	Soybean	cytosol	82.17	85.26
KRH63934	Soybean	endoplasmic reticulum	82.45	84.81
CDY20973	Canola	cytosol	83.57	84.75
Bra018417.1-P	Field mustard	cytosol	83.29	84.46
CDY34860	Canola	cytosol	83.57	84.03
Bra031740.1-P	Field mustard	cytosol	83.29	83.75
Bra019889.1-P	Field mustard	cytosol	83.57	83.57
AT1G60940.1	Thale cress	cytosol	83.84	83.38
CDX97923	Canola	cytosol	83.84	83.15
CDY34668	Canola	cytosol	83.57	83.1
AT1G10940.2	Thale cress	cytosol	84.12	81.4
Os04t0432000-02	Rice	cytosol	80.78	80.78
EES06943	Sorghum	cytosol	81.62	80.49
TraesCS2B01G320500.1	Wheat	cytosol	79.67	80.11
TraesCS2A01G303900.1	Wheat	cytosol	79.67	80.11
HORVU2Hr1G075470.2	Barley	cytosol	79.39	79.83
TraesCS2D01G302500.1	Wheat	cytosol, golgi	79.39	79.83
Os02t0551100-01	Rice	plasma membrane	80.22	78.9
CDY20594	Canola	cytosol	64.62	75.57
KXG26327	Sorghum	cytosol	78.55	74.8
CDY19332	Canola	cytosol	83.57	72.29
KRH33219	Soybean	cytosol	25.35	71.65
KRH43149	Soybean	cytosol	67.69	70.43
KRH57532	Soybean	cytosol, endoplasmic reticulum, nucleus	65.46	69.94
KRH28204	Soybean	cytosol	66.02	69.91
KRH72382	Soybean	cytosol	65.46	69.53
KRH04240	Soybean	cytosol	64.9	69.35
KRH77286	Soybean	cytosol	65.46	69.32
KRH43042	Soybean	cytosol	50.14	68.7
KRH71186	Soybean	endoplasmic reticulum	67.69	68.64
KRH16769	Soybean	cytosol	64.35	68.34
KRG89230	Soybean	cytosol	68.8	67.86
KRH28215	Soybean	cytosol, plastid, vacuole	22.84	67.77
KRH50231	Soybean	cytosol	68.8	67.3
KRH49772	Soybean	cytosol	69.36	67.3
Bra019890.1-P	Field mustard	cytosol	19.22	66.99
KRH59295	Soybean	cytosol, endoplasmic reticulum	58.77	66.98
KRH04679	Soybean	nucleus, plastid	66.3	66.11
KRH76942	Soybean	cytosol	55.15	65.56
KRH57751	Soybean	nucleus	65.74	65.56
KRH28516	Soybean	cytosol	63.79	63.79
CDX97922	Canola	cytosol	41.5	60.82
KRH76943	Soybean	cytosol, extracellular, plasma membrane	8.64	54.39
KRH08581	Soybean	mitochondrion	16.99	53.51
CDY03768	Canola	cytosol	85.24	51.95
KRH30137	Soybean	extracellular, plasma membrane	13.37	40.0

Protein Annotations

Gene3D:1.10.510.10	EntrezGene:100809324	MapMan:18.4.5.2	Gene3D:3.30.200.20	EMBL:ACUP02007709	ncoils:Coil
EnsemblPlantsGene:GLYMA_12G169800	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674
GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005737	GO:GO:0006464	GO:GO:0006468	GO:GO:0007154	GO:GO:0007165	GO:GO:0008150
GO:GO:0008152	GO:GO:0009987	GO:GO:0016301	GO:GO:0016740	GO:GO:0019538	GO:GO:0035556
UniProt:I1LTF6	InterPro:IPR000719	EnsemblPlants:KRH26363	ProteinID:KRH26363	ProteinID:KRH26363.1	InterPro:Kinase-like_dom_sf
PFAM:PF00069	PIRSF:PIRSF000654	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24343
PANTHER:PTHR24343:SF161	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS
UniParc:UPI000233D2F8	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr12:+:32462354..32467301

Molecular Weight (calculated)

41083.8 Da

IEP (calculated)

6.110

GRAVY (calculated)

-0.537

Length

359 amino acids

Sequence
(BLAST)

001: MDKYELVKDI GSGNFGVARL MRHKDTKELV AMKYIERGHK IDENVAREII NHRSLRHPNI IRFKEVVLTP THLGIVMEYA AGGELFERIC SAGRFSEDEA
101: RYFFQQLISG VSYCHSMQIC HRDLKLENTL LDGSPAPRLK ICDFGYSKSS LLHSRPKSTV GTPAYIAPEV LSRREYDGKL ADVWSCGVTL YVMLVGAYPF
201: EDQDDPKNFR KTINRIMAVQ YKIPDYVHIS QDCRHLLSRI FVANPARRIT IKEIKSHPWF LKNLPRELTE VAQAAYYRKE NPTFSLQSIE GIMNIVEEAK
301: TPPPASRSIG GFGWGGEEEE DETKEEEDET KEEAVTEAEE DEYEKRVKEA QASGEFHVS

Best Arabidopsis Sequence Match ( AT1G60940.2 )
(BLAST)

001: MDKYELVKDI GAGNFGVARL MRVKNSKELV AMKYIERGPK IDENVAREII NHRSLRHPNI IRFKEVVLTP THIAIAMEYA AGGELFERIC SAGRFSEDEA
101: RYFFQQLISG VSYCHAMQIC HRDLKLENTL LDGSPAPRLK ICDFGYSKSS LLHSMPKSTV GTPAYIAPEV LSRGEYDGKM ADVWSCGVTL YVMLVGAYPF
201: EDQEDPKNFK KTIQRIMAVK YKIPDYVHIS QDCKHLLSRI FVTNSNKRIT IGDIKKHPWF LKNLPRELTE IAQAAYFRKE NPTFSLQSVE EIMKIVEEAK
301: TPARVSRSIG AFGWGGGEDA EGKEEDAEEE VEEVEEEEDE EDEYDKTVKQ VHASMGEVRV S

Arabidopsis Description

SRK2BSerine/threonine-protein kinase SRK2B [Source:UniProtKB/Swiss-Prot;Acc:Q9C958]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.