CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 4
cytosol 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
EpiLoc:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH02667
KRH12454
KRH19237
KRH37441
KRH45453
KRH75621

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G66880.1	KRH45453	AT4G17870.1	19874541
AT5G66880.1	KRH75621	AT4G17870.1	19874541
AT5G66880.1	KRH02667	AT4G26080.1	19874541
AT5G66880.1	KRH12454	AT4G26080.1	19874541
AT5G66880.1	KRH19237	AT4G26080.1	19874541
AT5G66880.1	KRH37441	AT4G26080.1	19874541

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH04679	Soybean	nucleus, plastid	98.61	98.61
KRH76943	Soybean	cytosol, extracellular, plasma membrane	14.44	91.23
KRH28516	Soybean	cytosol	86.11	86.35
KRH76942	Soybean	cytosol	71.67	85.43
KRH28215	Soybean	cytosol, plastid, vacuole	28.33	84.3
Solyc02g090390.2.1	Tomato	cytosol	82.22	84.09
KRG89230	Soybean	cytosol	84.72	83.79
VIT_07s0197g00080.t01	Wine grape	cytosol	83.06	82.83
KRH71186	Soybean	endoplasmic reticulum	80.83	82.2
KRH49772	Soybean	cytosol	84.44	82.16
GSMUA_Achr4P28190_001	Banana	cytosol	82.78	82.09
Zm00001d013736_P001	Maize	cytosol	81.94	81.49
Bra012113.1-P	Field mustard	cytosol	80.56	81.23
AT5G66880.1	Thale cress	cytosol	81.39	81.16
EER91234	Sorghum	cytosol	81.39	80.94
KRH50231	Soybean	cytosol	82.5	80.93
Os03t0610900-01	Rice	cytosol	81.11	80.66
TraesCS4D01G078100.2	Wheat	cytosol	80.28	80.06
HORVU4Hr1G013540.3	Barley	cytosol	80.28	80.06
TraesCS4B01G079300.1	Wheat	cytosol	80.28	80.06
TraesCS4A01G235600.2	Wheat	cytosol	80.28	80.06
GSMUA_Achr4P23200_001	Banana	cytosol	81.39	79.84
HORVU5Hr1G097630.1	Barley	cytosol	80.83	79.51
EER93398	Sorghum	cytosol	80.83	79.51
TraesCS5D01G411900.1	Wheat	cytosol	80.56	79.23
TraesCS5B01G406400.1	Wheat	cytosol	80.56	79.23
TraesCS5A01G401700.1	Wheat	cytosol	80.56	79.23
KRH33219	Soybean	cytosol	27.78	78.74
Bra012897.1-P	Field mustard	cytosol	78.89	78.67
GSMUA_Achr2P13360_001	Banana	cytosol	79.72	78.63
Zm00001d033339_P002	Maize	cytosol, extracellular	79.44	78.57
Os03t0764800-01	Rice	plasma membrane	79.72	77.36
Os12t0586100-01	Rice	cytosol	77.5	77.29
AT3G50500.2	Thale cress	cytosol	78.89	76.96
Zm00001d013201_P003	Maize	cytosol	80.83	76.58
TraesCS5B01G075800.1	Wheat	cytosol	75.28	74.66
HORVU0Hr1G011570.1	Barley	cytosol	73.89	74.51
TraesCS5D01G081700.1	Wheat	cytosol	75.28	74.45
TraesCS5A01G069500.1	Wheat	cytosol	74.44	74.44
EES17292	Sorghum	cytosol	74.72	73.9
HORVU5Hr1G018340.2	Barley	cytosol	73.89	71.7
Zm00001d034161_P003	Maize	cytosol	78.89	71.18
KRH72382	Soybean	cytosol	63.06	67.16
KRH43149	Soybean	cytosol	63.89	66.67
KRH57532	Soybean	cytosol, endoplasmic reticulum, nucleus	62.22	66.67
KRH16769	Soybean	cytosol	62.5	66.57
KRH44077	Soybean	endoplasmic reticulum, nucleus	65.0	66.48
KRH04240	Soybean	cytosol	61.67	66.07
KRH59681	Soybean	endoplasmic reticulum	64.17	65.81
KRH28204	Soybean	cytosol	61.94	65.78
KRH26363	Soybean	nucleus	65.56	65.74
KRH54011	Soybean	cytosol	63.06	65.61
KRH41015	Soybean	endoplasmic reticulum	63.89	65.53
KRH77286	Soybean	cytosol	61.67	65.49
KRH63934	Soybean	endoplasmic reticulum	63.06	65.04
Zm00001d024775_P002	Maize	cytosol	19.17	63.89
KRH59295	Soybean	cytosol, endoplasmic reticulum	55.83	63.81
KRH43042	Soybean	cytosol	43.61	59.92
CDY52825	Canola	plasma membrane	25.0	53.89
KRH08581	Soybean	mitochondrion	15.28	48.25
Zm00001d020262_P003	Maize	cytosol	9.72	47.95
KRH30137	Soybean	extracellular, plasma membrane	13.06	39.17
Zm00001d011604_P003	Maize	mitochondrion	31.94	24.21

Protein Annotations

Gene3D:1.10.510.10	EntrezGene:100801175	MapMan:18.4.5.2	Gene3D:3.30.200.20	EMBL:ACUP02002898	EnsemblPlantsGene:GLYMA_05G081900
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005737
GO:GO:0006464	GO:GO:0006468	GO:GO:0007154	GO:GO:0007165	GO:GO:0008150	GO:GO:0008152
GO:GO:0009987	GO:GO:0016301	GO:GO:0016740	GO:GO:0019538	GO:GO:0035556	UniProt:I1K1R0
InterPro:IPR000719	EnsemblPlants:KRH57751	ProteinID:KRH57751	ProteinID:KRH57751.1	InterPro:Kinase-like_dom_sf	PFAM:PF00069
PIRSF:PIRSF000654	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24343	PANTHER:PTHR24343:SF162
InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI0002339F5E

Description

hypothetical protein

Coordinates

chr5:+:12660412..12665851

Molecular Weight (calculated)

40803.6 Da

IEP (calculated)

4.504

GRAVY (calculated)

-0.268

Length

360 amino acids

Sequence
(BLAST)

001: MDRAALTVGP GMDMPIMHDS DRYDLVRDIG SGNFGVARLM QDKQTKELVA VKYIERGDKI DENVKREIIN HRSLRHPNIV RFKEVILTPT HLAIVMEYAS
101: GGELFEKICN AGRFTEDEAR FFFQQLISGV SYCHAMQVCH RDLKLENTLL DGSSAPRLKI CDFGYSKSSV LHSQPKSTVG TPAYIAPEVL LKQEYDGKLA
201: DVWSCGVTLY VMLVGAYPFE DPNEPKDFRK TIQRVLSVQY SIPDGVQISP ECGHLISRIF VFDPAERITM SEIWNHEWFL KNLPADLMDE KIMSNQFEEP
301: DQPMQSIDTI MQIISEATVP AAGTYSFDKF MEEQIYDLES ESDAESDLDI DSSGEIVYAI

Best Arabidopsis Sequence Match ( AT5G66880.1 )
(BLAST)

001: MDRAPVTTGP LDMPIMHDSD RYDFVKDIGS GNFGVARLMR DKLTKELVAV KYIERGDKID ENVQREIINH RSLRHPNIVR FKEVILTPTH LAIIMEYASG
101: GELYERICNA GRFSEDEARF FFQQLLSGVS YCHSMQICHR DLKLENTLLD GSPAPRLKIC DFGYSKSSVL HSQPKSTVGT PAYIAPEVLL RQEYDGKIAD
201: VWSCGVTLYV MLVGAYPFED PEEPRDYRKT IQRILSVKYS IPDDIRISPE CCHLISRIFV ADPATRISIP EIKTHSWFLK NLPADLMNES NTGSQFQEPE
301: QPMQSLDTIM QIISEATIPA VRNRCLDDFM TDNLDLDDDM DDFDSESEID IDSSGEIVYA L

Arabidopsis Description

SRK2ISerine/threonine-protein kinase SRK2I [Source:UniProtKB/Swiss-Prot;Acc:Q39193]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.