CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

nucleus 3
cytosol 2
mitochondrion 1
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH13399
KRH20755
KRH62918

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G60940.1	KRH13399	AT5G13810.1	21798944
AT1G60940.1	KRH20755	AT5G13810.1	21798944
AT1G60940.1	KRH62918	AT5G13810.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH26363	Soybean	nucleus	98.01	96.1
VIT_12s0035g00310.t01	Wine grape	cytosol	91.48	90.45
PGSC0003DMT400061170	Potato	cytosol	89.77	87.78
Solyc01g103940.2.1	Tomato	plastid	89.77	87.53
Bra031475.1-P	Field mustard	cytosol	87.22	87.22
CDY46190	Canola	cytosol	87.22	87.22
KRH59681	Soybean	endoplasmic reticulum	84.66	84.9
KRH41015	Soybean	endoplasmic reticulum	84.66	84.9
KRH54011	Soybean	cytosol	82.95	84.39
CDY20973	Canola	cytosol	84.66	84.18
KRH63934	Soybean	endoplasmic reticulum	83.24	83.95
Bra018417.1-P	Field mustard	cytosol	84.38	83.9
CDY34860	Canola	cytosol	84.94	83.75
Bra019889.1-P	Field mustard	cytosol	85.23	83.57
Bra031740.1-P	Field mustard	cytosol	84.66	83.47
CDX97923	Canola	cytosol	85.8	83.43
CDY34668	Canola	cytosol	84.94	82.83
AT1G60940.1	Thale cress	cytosol	84.66	82.55
AT1G10940.2	Thale cress	cytosol	84.94	80.59
Os04t0432000-02	Rice	cytosol	81.82	80.22
TraesCS2B01G320500.1	Wheat	cytosol	80.97	79.83
TraesCS2A01G303900.1	Wheat	cytosol	80.97	79.83
EES06943	Sorghum	cytosol	82.39	79.67
TraesCS2D01G302500.1	Wheat	cytosol, golgi	80.68	79.55
HORVU2Hr1G075470.2	Barley	cytosol	80.68	79.55
Os02t0551100-01	Rice	plasma membrane	81.53	78.63
CDY20594	Canola	cytosol	66.48	76.22
KXG26327	Sorghum	cytosol	79.55	74.27
CDY19332	Canola	cytosol	85.23	72.29
KRH33219	Soybean	cytosol	25.85	71.65
KRH43149	Soybean	cytosol	69.32	70.72
KRH28204	Soybean	cytosol	67.9	70.5
KRH57532	Soybean	cytosol, endoplasmic reticulum, nucleus	67.05	70.24
KRH77286	Soybean	cytosol	67.33	69.91
KRH04240	Soybean	cytosol	66.48	69.64
KRH72382	Soybean	cytosol	66.76	69.53
KRH16769	Soybean	cytosol	65.91	68.64
KRH71186	Soybean	endoplasmic reticulum	68.75	68.36
KRH43042	Soybean	cytosol	50.85	68.32
Bra019890.1-P	Field mustard	cytosol	19.89	67.96
KRH28215	Soybean	cytosol, plastid, vacuole	23.3	67.77
KRG89230	Soybean	cytosol	69.89	67.58
KRH59295	Soybean	cytosol, endoplasmic reticulum	60.23	67.3
KRH50231	Soybean	cytosol	69.89	67.03
KRH49772	Soybean	cytosol	70.45	67.03
KRH04679	Soybean	nucleus, plastid	67.05	65.56
KRH76942	Soybean	cytosol	55.97	65.23
KRH57751	Soybean	nucleus	66.48	65.0
KRH28516	Soybean	cytosol	64.49	63.23
CDX97922	Canola	cytosol	42.61	61.22
KRH08581	Soybean	mitochondrion	17.61	54.39
KRH76943	Soybean	cytosol, extracellular, plasma membrane	8.52	52.63
CDY03768	Canola	cytosol	86.93	51.95
KRH30137	Soybean	extracellular, plasma membrane	13.35	39.17

Protein Annotations

Gene3D:1.10.510.10	EntrezGene:100812259	MapMan:18.4.5.2	Gene3D:3.30.200.20	EMBL:ACUP02004908	ncoils:Coil
EnsemblPlantsGene:GLYMA_08G188300	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674
GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005737	GO:GO:0006464	GO:GO:0006468	GO:GO:0007154	GO:GO:0007165	GO:GO:0008150
GO:GO:0008152	GO:GO:0009987	GO:GO:0016301	GO:GO:0016740	GO:GO:0019538	GO:GO:0035556
UniProt:I1KUN7	InterPro:IPR000719	EnsemblPlants:KRH44077	ProteinID:KRH44077	ProteinID:KRH44077.1	ProteinID:KRH44078.1
InterPro:Kinase-like_dom_sf	PFAM:PF00069	PIRSF:PIRSF000654	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011
PANTHER:PTHR24343	PANTHER:PTHR24343:SF161	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF56112
InterPro:Ser/Thr_kinase_AS	UniParc:UPI0000E64848	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr8:-:15104067..15107672

Molecular Weight (calculated)

40265.0 Da

IEP (calculated)

6.516

GRAVY (calculated)

-0.486

Length

352 amino acids

Sequence
(BLAST)

001: MEKYELVKDI GSGNFGVARL MRHKDTKELV AMKYIERGHK IDENVAREII NHRSLRHPNI IRFKEVVLTP THLGIVMEYA AGGELFERIC SAGRFSEDEA
101: RYFFQQLISG VSYCHSMQIC HRDLKLENTL LDGSPAPRLK ICDFGYSKSS LLHSRPKSTV GTPAYIAPEV LSRREYDGKL ADVWSCGVTL YVMLVGAYPF
201: EDQEDPKNFR KTINRIMAVQ YKIPDYVHIS QDCRHLLSRI FVANPARRIT IKEIKSHPWF VKNLPRELTE VAQAAYYRKE NPTFSLQSIE DIMNIVEEAK
301: APPPASRSIG GFGWGGEEEE EDETKEAVAE TEEDEYEKRV KEAQASGEFH VS

Best Arabidopsis Sequence Match ( AT1G60940.2 )
(BLAST)

001: MDKYELVKDI GAGNFGVARL MRVKNSKELV AMKYIERGPK IDENVAREII NHRSLRHPNI IRFKEVVLTP THIAIAMEYA AGGELFERIC SAGRFSEDEA
101: RYFFQQLISG VSYCHAMQIC HRDLKLENTL LDGSPAPRLK ICDFGYSKSS LLHSMPKSTV GTPAYIAPEV LSRGEYDGKM ADVWSCGVTL YVMLVGAYPF
201: EDQEDPKNFK KTIQRIMAVK YKIPDYVHIS QDCKHLLSRI FVTNSNKRIT IGDIKKHPWF LKNLPRELTE IAQAAYFRKE NPTFSLQSVE EIMKIVEEAK
301: TPARVSRSIG AFGWGGGEDA EGKEEDAEEE VEEVEEEEDE EDEYDKTVKQ VHASMGEVRV S

Arabidopsis Description

SRK2BSerine/threonine-protein kinase SRK2B [Source:UniProtKB/Swiss-Prot;Acc:Q9C958]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.