CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1
nucleus 3
plastid 3
mitochondrion 1
cytosol 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus, plastid, secretory
BaCelLo:	secretory
EpiLoc:	nucleus
iPSORT:	plastid
MultiLoc:	nucleus
Plant-mPloc:	nucleus, plastid
PProwler:	mitochondrion
TargetP:	plastid
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91301
KRG97345
KRH02428
KRH31570
KRH35520
KRH50633

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G21215.1	KRH02428	AT3G17310.1	21798944
AT3G21215.1	KRH50633	AT3G17310.1	21798944
AT3G21215.1	KRG91301	AT3G22440.1	21798944
AT3G21215.1	KRH35520	AT3G22440.1	21798944
AT3G21215.1	KRG97345	AT4G13850.1	21798944
AT3G21215.1	KRH31570	AT4G13850.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH47009	Soybean	nucleus	88.96	80.57
CDY37307	Canola	nucleus	52.37	78.3
VIT_09s0018g01880.t01	Wine grape	nucleus	73.5	77.93
Bra023895.1-P	Field mustard	nucleus	69.09	71.57
GSMUA_Achr2P05000_001	Banana	cytosol	66.88	70.2
GSMUA_Achr9P19610_001	Banana	cytosol	65.93	68.3
AT3G21215.1	Thale cress	nucleus	72.24	67.55
Solyc03g123840.2.1	Tomato	nucleus	68.14	67.5
PGSC0003DMT400023680	Potato	plastid	68.14	65.06
CDY27533	Canola	nucleus	70.35	65.01
TraesCS7A01G287800.1	Wheat	nucleus, plastid	64.67	64.26
TraesCS7D01G290200.1	Wheat	nucleus, plastid	64.35	63.95
Zm00001d049346_P001	Maize	plastid	64.35	63.35
TraesCS7B01G182400.1	Wheat	nucleus, plastid	63.72	63.32
EES14795	Sorghum	plastid	63.72	63.12
HORVU7Hr1G067800.6	Barley	plastid	64.35	59.3
CDY06428	Canola	nucleus	48.58	49.84
KRH47143	Soybean	nucleus	25.24	30.19
KRH44168	Soybean	nucleus	25.55	29.24
KRH28438	Soybean	nucleus	28.71	26.92
KRH77037	Soybean	nucleus	26.81	25.53

Protein Annotations

Gene3D:3.30.70.330	MapMan:35.2	EMBL:ACUP02004945	EnsemblPlantsGene:GLYMA_08G223200	GO:GO:0003674	GO:GO:0003676
GO:GO:0003723	GO:GO:0005488	InterPro:IPR000504	InterPro:IPR012677	UniProt:K7L852	EnsemblPlants:KRH44644
ProteinID:KRH44644	ProteinID:KRH44644.1	InterPro:Nucleotide-bd_a/b_plait_sf	PFAM:PF00076	PFscan:PS50102	PANTHER:PTHR45381
InterPro:RBD_domain_sf	InterPro:RRM_dom	SMART:SM00360	SUPFAM:SSF54928	UniParc:UPI00023C60A9	SEG:seg

Description

hypothetical protein

Coordinates

chr8:-:18103030..18104695

Molecular Weight (calculated)

34815.8 Da

IEP (calculated)

6.703

GRAVY (calculated)

-0.304

Length

317 amino acids

Sequence
(BLAST)

001: MAGTGIHPYH QQWAPAAAAP PPPPPAAAGG PPPHPGEEVR TIFITGLPED VKERELQNLL RWLPGFEASQ LNFKAEKPMG FALFSAPHQA LTAKDILQDM
101: LFDPDTKSVL HTEMAKKNLF VKRGIGADAG AFDQSKRLRT AGDYTHTGYT SPSPFHPPPP PVWGPHGYMA PPPPPPYDPY AGYPVAPVPM PTPAPIAAPS
201: TYVPVQNTKD NPPCNTLFIG NLGENINEEE VRGLFSVQPG FKQMKILRQE RHTVCFIEFE DVNSATNVHH NLQGAVIPSS GSIGMRIQYP FLYIINMINH
301: PDVCFLIFPP HMFPFLN

Best Arabidopsis Sequence Match ( AT3G21215.3 )
(BLAST)

001: MAGAGIHPYH QQWPPAGAPP PPAAVSSAAP PHPPPIHHHP PPPPVLVDNH NRPPYDELRT IFIAGLPDDV KERELLNLLR WLPGYEASQV NFKGEKPMGF
101: ALFSTAQFAM AAKDTLQHMV FDAESKSVIH TEMAKKNLFV KRGIVGDSNA YDQSKRLRTG GDCTHSVYSP SPFHPPPPPV WGPPHGYMAP APPPYDPYAG
201: YHAPPVPMPT PPPIAAPSSY VPVQNIKDNP PCNTLFIGNL GENINEEELR SLLSAQPGFK QMKILRQERH TVCFIEFEDV NSATNVHHNL QGAVIPSSGS
301: IGMRIQYPYI LLLFLII

Arabidopsis Description

AT3G21215 protein [Source:UniProtKB/TrEMBL;Acc:Q9ASW4]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.