CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 2
plastid 3
cytosol 3
mitochondrion 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89812
KRG94975
KRH18664
KRH31638
KRH69070

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G23650.1	KRG89812	AT2G41090.1	17360592
AT4G23650.1	KRG94975	AT2G41090.1	17360592
AT4G23650.1	KRH18664	AT2G41090.1	17360592
AT4G23650.1	KRG89812	AT2G41100.1	17360592
AT4G23650.1	KRG94975	AT2G41100.1	17360592
AT4G23650.1	KRH18664	AT2G41100.1	17360592
AT4G23650.1	KRH31638	AT4G14640.1	17360592
AT4G23650.1	KRH69070	AT4G14640.1	17360592
AT4G23650.1	KRG89812	AT5G21274.1	17360592
AT4G23650.1	KRG94975	AT5G21274.1	17360592
AT4G23650.1	KRH18664	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH27875	Soybean	nucleus	86.1	88.32
KRH77599	Soybean	nucleus	85.71	84.09
GSMUA_Achr6P31900_001	Banana	mitochondrion	69.88	83.03
VIT_02s0025g00690.t01	Wine grape	cytosol	84.56	82.95
Solyc08g008170.2.1	Tomato	plastid	81.66	81.98
PGSC0003DMT400014939	Potato	plastid	81.27	81.43
TraesCS1B01G456700.1	Wheat	cytosol	71.43	80.43
TraesCS1A01G424200.1	Wheat	cytosol	71.24	80.22
TraesCS1D01G433400.1	Wheat	cytosol, golgi	71.24	80.22
HORVU1Hr1G092710.1	Barley	cytosol, plasma membrane	73.55	79.38
CDY10955	Canola	cytosol	56.76	78.19
Bra019282.1-P	Field mustard	cytosol	56.37	77.66
GSMUA_Achr9P05370_001	Banana	cytosol	66.41	77.13
Os01t0622600-01	Rice	plasma membrane	77.03	77.03
GSMUA_Achr2P06360_001	Banana	plasma membrane	76.64	76.94
KRH41261	Soybean	cytosol	63.9	75.74
TraesCS3D01G228700.1	Wheat	cytosol, mitochondrion	75.1	75.1
TraesCS3B01G254500.1	Wheat	golgi, unclear	75.1	75.1
TraesCS3A01G224900.1	Wheat	cytosol, mitochondrion	75.1	75.1
GSMUA_Achr1P05040_001	Banana	cytosol	68.53	74.89
EES01094	Sorghum	cytosol, mitochondrion	75.87	74.86
Zm00001d011392_P001	Maize	cytosol, plastid	76.06	74.34
Zm00001d044185_P005	Maize	cytosol, mitochondrion	74.9	74.05
Zm00001d009708_P001	Maize	mitochondrion	77.03	74.03
EES20022	Sorghum	cytosol, mitochondrion	76.64	73.38
AT4G23650.1	Thale cress	plastid	74.13	72.59
CDY54853	Canola	plastid	73.55	72.57
Os05t0585500-01	Rice	plasma membrane	75.87	72.51
Bra013719.1-P	Field mustard	cytosol, plastid	73.36	72.38
CDY44125	Canola	cytosol, plastid	73.17	72.19
Bra019281.1-P	Field mustard	plastid	72.97	71.05
Bra019284.1-P	Field mustard	plastid	72.97	71.05
KRG89696	Soybean	cytosol, mitochondrion	69.88	66.3
KRH50623	Soybean	cytosol	69.11	66.17
KRH73535	Soybean	cytosol	67.18	66.03
KRH14593	Soybean	cytosol	67.37	65.97
HORVU3Hr1G055730.3	Barley	plastid	75.29	65.66
KRH73749	Soybean	nucleus	65.83	64.58
KRH14398	Soybean	cytosol, nucleus	66.41	63.82
KRH02072	Soybean	endoplasmic reticulum, nucleus	64.67	62.27
KRH51083	Soybean	endoplasmic reticulum	63.32	62.0
CDY62741	Canola	endoplasmic reticulum, plasma membrane	73.17	61.63
KRH46181	Soybean	nucleus	64.67	60.8
KRG98764	Soybean	cytosol	64.48	60.62
KRG91803	Soybean	mitochondrion	57.14	60.41
KRH34984	Soybean	cytosol	57.34	60.37
KRH17481	Soybean	cytosol	62.36	60.15
KRH54032	Soybean	cytosol	57.34	59.76
KRH63919	Soybean	cytosol	56.95	59.48
KRH41022	Soybean	plastid	56.56	57.68
KRH59698	Soybean	plastid	56.37	57.59
KRH74107	Soybean	cytosol	60.62	57.19
KRH34983	Soybean	cytosol	52.7	56.64
KRH05995	Soybean	cytosol	55.79	56.34
KRH05994	Soybean	cytosol	57.92	55.97
KRH14008	Soybean	cytosol	60.23	55.91
KRG90147	Soybean	cytosol, plastid	61.39	54.92
KRH33395	Soybean	endoplasmic reticulum, nucleus	60.81	54.22
KRH72128	Soybean	plastid	57.92	51.11
KRH32931	Soybean	plastid	57.72	50.25
KRH68038	Soybean	cytosol	56.76	49.83
KRG96293	Soybean	cytosol	56.18	49.07

Protein Annotations

Gene3D:1.10.238.10	Gene3D:1.10.510.10	EntrezGene:100777096	MapMan:18.4.5.4	Gene3D:3.30.200.20	EMBL:ACUP02003347
InterPro:EF-hand-dom_pair	InterPro:EF_Hand_1_Ca_BS	InterPro:EF_hand_dom	EnsemblPlantsGene:GLYMA_05G213200	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0004672	GO:GO:0004683	GO:GO:0005488	GO:GO:0005509	GO:GO:0005515
GO:GO:0005516	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005737	GO:GO:0006464	GO:GO:0006468	GO:GO:0007154	GO:GO:0007165	GO:GO:0008150
GO:GO:0008152	GO:GO:0009719	GO:GO:0009738	GO:GO:0009931	GO:GO:0009987	GO:GO:0016301
GO:GO:0016740	GO:GO:0018105	GO:GO:0019538	GO:GO:0035556	GO:GO:0046777	UniProt:I1K6C6
InterPro:IPR000719	InterPro:IPR002048	EnsemblPlants:KRH59992	ProteinID:KRH59992	ProteinID:KRH59992.1	InterPro:Kinase-like_dom_sf
PFAM:PF00069	PFAM:PF13499	ScanProsite:PS00018	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011
PFscan:PS50222	PANTHER:PTHR24349	PANTHER:PTHR24349:SF182	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00054
SMART:SM00220	SUPFAM:SSF47473	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI00023396E6	SEG:seg

Description

hypothetical protein

Coordinates

chr5:-:39468820..39475106

Molecular Weight (calculated)

58254.6 Da

IEP (calculated)

6.375

GRAVY (calculated)

-0.444

Length

518 amino acids

Sequence
(BLAST)

001: MGNCSSGAGA PTTYSDDHPH HNGITVLPPN SNPSPQLPKP PPTSSSSSSL GRVLGRPMED VRSIYIFGRE LGRGQFGVTY LVTHKATKEQ FACKSIATRK
101: LVNRDDIDDI RREVQIMHHL TGHRNIVELK GAYEDRHSVN LVMELCAGGE LFDRIITKGH YSERAAANSC RQIVTVVHNC HSMGVMHRDL KPENFLLLNK
201: NDDSPLKATD FGLSVFFKPG DVFRDLVGSA YYVAPEVLRR SYGPEADIWS AGVILYILLS GVPPFWAENE QGIFDAILRG HIDFASDPWP SISSSAKDLV
301: KKMLRADPKE RLSAVEVLNH PWMRVDGDAP DKPLDIAVLT RMKQFRAMNK LKKVALKVIA ENLSEEEIIG LKEMFKSMDT DNSGTITFEE LKAGLPKLGT
401: KLSESEVRQL MEAADVDGNG TIDYIEFITA TMHMNRMERE DHLYKAFEYF DNDKSGYITM EELESALKKY NMGDEKTIKE IIAEVDTDND GRINYDEFVA
501: MMRKGNPDIT HITQRRRK

Best Arabidopsis Sequence Match ( AT4G23650.1 )
(BLAST)

001: MGHRHSKSKS SDPPPSSSSS SSGNVVHHVK PAGERRGSSG SGTVGSSGSG TGGSRSTTST QQNGRILGRP MEEVRRTYEF GRELGRGQFG VTYLVTHKET
101: KQQVACKSIP TRRLVHKDDI EDVRREVQIM HHLSGHRNIV DLKGAYEDRH SVNLIMELCE GGELFDRIIS KGLYSERAAA DLCRQMVMVV HSCHSMGVMH
201: RDLKPENFLF LSKDENSPLK ATDFGLSVFF KPGDKFKDLV GSAYYVAPEV LKRNYGPEAD IWSAGVILYI LLSGVPPFWG ENETGIFDAI LQGQLDFSAD
301: PWPALSDGAK DLVRKMLKYD PKDRLTAAEV LNHPWIREDG EASDKPLDNA VLSRMKQFRA MNKLKKMALK VIAENLSEEE IIGLKEMFKS LDTDNNGIVT
401: LEELRTGLPK LGSKISEAEI RQLMEAADMD GDGSIDYLEF ISATMHMNRI EREDHLYTAF QFFDNDNSGY ITMEELELAM KKYNMGDDKS IKEIIAEVDT
501: DRDGKINYEE FVAMMKKGNP ELVPNRRRM

Arabidopsis Description

CPK3CPK3 [Source:UniProtKB/TrEMBL;Acc:A0A178V4I3]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.