CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

plastid 6
cytosol 3
nucleus 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH08775
KRH08776
KRH38307
KRH38311

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G10660.1	KRH08775	AT5G24030.1	24280384
AT3G10660.1	KRH08776	AT5G24030.1	24280384
AT3G10660.1	KRH38307	AT5G24030.1	24280384
AT3G10660.1	KRH38311	AT5G24030.1	24280384

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG90147	Soybean	cytosol, plastid	97.93	98.27
VIT_13s0175g00080.t01	Wine grape	cytosol, plastid	83.3	85.06
PGSC0003DMT400002592	Potato	plastid	81.76	82.18
Solyc11g006370.1.1	Tomato	plastid	81.41	81.83
Solyc04g009800.2.1	Tomato	cytosol, plastid	80.38	80.38
PGSC0003DMT400065419	Potato	plastid	80.03	80.03
CDX70192	Canola	cytosol, plastid	76.94	78.15
CDX98791	Canola	cytosol, plastid	76.76	77.84
Bra009420.1-P	Field mustard	cytosol, plastid	78.14	76.82
CDX80925	Canola	cytosol	75.73	76.79
GSMUA_Achr7P07810_001	Banana	cytosol, plastid	66.44	76.74
CDY08584	Canola	cytosol	75.39	76.04
CDY11313	Canola	cytosol	76.76	75.85
GSMUA_AchrUn_...	Banana	cytosol	73.32	75.8
AT5G04870.1	Thale cress	cytosol, plastid	79.52	75.74
Bra005824.1-P	Field mustard	cytosol, plastid	78.14	74.92
CDY00963	Canola	plastid	73.67	74.56
CDX73914	Canola	plastid	75.9	73.62
CDY00688	Canola	plastid	75.9	73.5
KRH72128	Soybean	plastid	73.84	73.08
Os03t0788500-01	Rice	cytosol	72.98	70.78
KRH63919	Soybean	cytosol	60.24	70.56
KRH54032	Soybean	cytosol	59.72	69.82
AT3G10660.1	Thale cress	cytosol, plastid	77.45	69.66
KRG91803	Soybean	mitochondrion	58.69	69.59
EER93328	Sorghum	cytosol	73.84	69.53
KRH34984	Soybean	cytosol	58.86	69.51
Zm00001d034372_P001	Maize	cytosol	73.15	69.44
KRH32931	Soybean	plastid	70.22	68.57
Zm00001d040996_P003	Maize	plastid	71.26	67.98
KXG23457	Sorghum	plastid	71.77	67.69
TraesCS5D01G434500.2	Wheat	cytosol	72.63	67.63
TraesCS5B01G428400.2	Wheat	cytosol	72.46	67.15
TraesCS5A01G426500.1	Wheat	cytosol	72.46	67.15
KRH59698	Soybean	plastid	58.18	66.67
TraesCS5B01G109300.1	Wheat	plastid	71.6	66.67
KRH41022	Soybean	plastid	58.18	66.54
TraesCS5D01G124000.1	Wheat	plastid	71.43	66.51
KRH74107	Soybean	cytosol	62.82	66.48
GSMUA_Achr4P22740_001	Banana	cytosol, plastid	73.67	66.46
Zm00001d013109_P002	Maize	plasma membrane	70.91	66.45
KRH68038	Soybean	cytosol	66.09	65.08
KRH14008	Soybean	cytosol	62.48	65.05
KRH34983	Soybean	cytosol	53.87	64.94
KRG96293	Soybean	cytosol	65.23	63.91
GSMUA_Achr7P01360_001	Banana	cytosol	72.63	62.89
KRH27875	Soybean	nucleus	54.04	62.18
KRH59992	Soybean	endoplasmic reticulum	54.22	60.81
HORVU5Hr1G030810.1	Barley	cytosol	71.43	60.23
KRH77599	Soybean	nucleus	53.53	58.9
KRH14593	Soybean	cytosol	51.64	56.71
KRH73535	Soybean	cytosol	51.29	56.55
KRH73749	Soybean	nucleus	50.77	55.87
KRG89696	Soybean	cytosol, mitochondrion	52.5	55.86
KRH50623	Soybean	cytosol	51.81	55.64
KRH14398	Soybean	cytosol, nucleus	50.95	54.92
KRG98764	Soybean	cytosol	51.64	54.45
KRH46181	Soybean	nucleus	51.29	54.08
KRH02072	Soybean	endoplasmic reticulum, nucleus	49.91	53.9
KRH51083	Soybean	endoplasmic reticulum	49.05	53.88
KRH17481	Soybean	cytosol	48.88	52.89
KRH41261	Soybean	cytosol	39.59	52.63
KRH05995	Soybean	cytosol	45.96	52.05
Os12t0486600-00	Rice	plasma membrane	67.47	50.84
KRH05994	Soybean	cytosol	43.89	47.57
Bra001373.1-P	Field mustard	plastid	77.8	46.6
Bra029859.1-P	Field mustard	plastid	75.73	46.32
CDY00964	Canola	plastid	0.52	0.9
CDY00689	Canola	plastid	0.34	0.6

Protein Annotations

Gene3D:1.10.238.10	Gene3D:1.10.510.10	EntrezGene:100819792	MapMan:18.4.5.4	Gene3D:3.30.200.20	UniProt:A0A0R0HX26
EMBL:ACUP02006475	InterPro:EF-hand-dom_pair	InterPro:EF_Hand_1_Ca_BS	InterPro:EF_hand_dom	EnsemblPlantsGene:GLYMA_10G120300	GO:GO:0000166
GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0005488	GO:GO:0005509	GO:GO:0005524
GO:GO:0006464	GO:GO:0006468	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301
GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:IPR002048	ProteinID:KRH33394.1	EnsemblPlants:KRH33395
ProteinID:KRH33395	ProteinID:KRH33395.1	InterPro:Kinase-like_dom_sf	PFAM:PF00069	PFAM:PF13499	ScanProsite:PS00018
ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PFscan:PS50222	PANTHER:PTHR24349	PANTHER:PTHR24349:SF161
InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00054	SMART:SM00220	SUPFAM:SSF47473	SUPFAM:SSF56112
InterPro:Ser/Thr_kinase_AS	UniParc:UPI0001B63BDE	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr10:-:30683200..30690502

Molecular Weight (calculated)

65044.6 Da

IEP (calculated)

5.295

GRAVY (calculated)

-0.398

Length

581 amino acids

Sequence
(BLAST)

001: MGNTCVGPSI SKNGFVQSVS AAIWRSQLPE GSVSNRESVK EEATSVPESP LPVQNKPPEQ ITMPKQEAKQ EGKSEIEPEK EQDKKKQKKR GSVKRVSSAG
101: LRVDSVLQRE TDNFKEFFTL GRKLGQGQFG TTFLCVEKAT GQEYACKSIA KRKLVTDDDV EDVRREIQIM HHLAGHPNVI SIKGAYEDAV AVHVVMELCA
201: GGELFDRIIQ RGHYTERQAA KLTKTIVGVV EACHSLGVMH RDLKPENFLF VNQHEDSLLK TIDFGLSVFF KPGDIFNDVV GSPYYVAPDV LRKRYGPEAD
301: VWSAGVILYI LLSGVPPFWA ENEQGIFEQV LRGDLDFSSD PWPSISESAK DLVRKMLVRD PRRRLTAHQV LCHPWIQVDG VAPDKPLDSA VLSRLKQFSA
401: MNKLKKMALI IIAESLSEEE IAGLKEMFKM IDADNSGQIT FEELKAGLKR VGANLKESEI YDLMQAADVD NSGTIDYGEF LAATLHRNKI EREDNLFAAF
501: SYFDKDGSGY ITQEELQQAC DEFGIKDVRL EEIIKEIDED NDGRIDYNEF VAMMQKGNLP AVGKKGLENS FSVKFREALK L

Best Arabidopsis Sequence Match ( AT5G04870.1 )
(BLAST)

001: MGNTCVGPSR NGFLQSVSAA MWRPRDGDDS ASMSNGDIAS EAVSGELRSR LSDEVQNKPP EQVTMPKPGT DVETKDREIR TESKPETLEE ISLESKPETK
101: QETKSETKPE SKPDPPAKPK KPKHMKRVSS AGLRTESVLQ RKTENFKEFY SLGRKLGQGQ FGTTFLCVEK TTGKEFACKS IAKRKLLTDE DVEDVRREIQ
201: IMHHLAGHPN VISIKGAYED VVAVHLVMEC CAGGELFDRI IQRGHYTERK AAELTRTIVG VVEACHSLGV MHRDLKPENF LFVSKHEDSL LKTIDFGLSM
301: FFKPDDVFTD VVGSPYYVAP EVLRKRYGPE ADVWSAGVIV YILLSGVPPF WAETEQGIFE QVLHGDLDFS SDPWPSISES AKDLVRKMLV RDPKKRLTAH
401: QVLCHPWVQV DGVAPDKPLD SAVLSRMKQF SAMNKFKKMA LRVIAESLSE EEIAGLKEMF NMIDADKSGQ ITFEELKAGL KRVGANLKES EILDLMQAAD
501: VDNSGTIDYK EFIAATLHLN KIEREDHLFA AFTYFDKDGS GYITPDELQQ ACEEFGVEDV RIEELMRDVD QDNDGRIDYN EFVAMMQKGS ITGGPVKMGL
601: EKSFSIALKL

Arabidopsis Description

CPK1CPK1 [Source:UniProtKB/TrEMBL;Acc:A0A178UQJ5]
SUBAcon: [plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.