Factsheet for Os12t0125300-01

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 4
cytosol 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	nucleus

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0829900-01
Os04t0486500-01	cytoskeleton, nucleus
Os11t0128700-00
Os12t0125300-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G05510.1	Os02t0829900-01	AT2G22640.1	25262777
AT5G05510.1	Os04t0486500-01	AT3G25980.1	25262777
AT5G05510.1	Os04t0486500-01	AT3G25980.1	25262777
AT5G05510.1	Os11t0128700-00	AT5G05510.1	25262777
AT5G05510.1	Os12t0125300-01	AT5G05510.1	25262777

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Os11t0128700-00	Rice	nucleus	96.3	96.46
TraesCS5A01G142400.1	Wheat	nucleus	66.56	66.77
EES09179	Sorghum	nucleus	65.92	66.34
HORVU7Hr1G029580.3	Barley	nucleus	66.08	66.18
TraesCSU01G188700.1	Wheat	nucleus	65.59	65.81
HORVU5Hr1G045460.1	Barley	mitochondrion	66.08	63.52
TraesCS5D01G149700.1	Wheat	plastid	65.92	62.22
TraesCS5B01G141100.1	Wheat	nucleus, plastid	65.59	61.82
Zm00001d023279_P001	Maize	nucleus	63.34	61.56
EES16550	Sorghum	mitochondrion, nucleus	66.72	57.96
Bra009117.1-P	Field mustard	nucleus	34.08	46.8
CDX70146	Canola	nucleus	33.6	46.14
CDX98847	Canola	nucleus	32.48	45.19
GSMUA_Achr5P14270_001	Banana	cytosol	38.75	44.8
AT5G05510.1	Thale cress	nucleus	32.8	43.31
KRH31175	Soybean	nucleus	34.41	40.68
KRG97697	Soybean	nucleus	33.92	40.42
Solyc06g051040.2.1	Tomato	cytosol, nucleus, plastid	35.69	37.76
VIT_13s0067g01530.t01	Wine grape	nucleus	35.05	36.03
Os02t0193600-01	Rice	nucleus	9.32	14.57
Os07t0508500-01	Rice	cytosol	9.65	10.71

Protein Annotations

Gene3D:1.25.40.430	MapMan:13.3.5.5.5	EntrezGene:4351380	ProteinID:ABA96340.1	ProteinID:BAF29062.1	ProteinID:BAT15687.1
InterPro:Bub1/Mad3	ncoils:Coil	ProteinID:EAZ19504.1	GO:GO:0000003	GO:GO:0000778	GO:GO:0003674
GO:GO:0003824	GO:GO:0004672	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0006464	GO:GO:0006468	GO:GO:0007049	GO:GO:0007094	GO:GO:0008150	GO:GO:0008152
GO:GO:0009987	GO:GO:0016043	GO:GO:0016301	GO:GO:0016310	GO:GO:0016740	GO:GO:0019538
GO:GO:0051754	InterPro:IPR013212	InterPro:Mad3/Bub1_I	EnsemblPlantsGene:Os12g0125300	EnsemblPlants:Os12t0125300-01	PFAM:PF08311
PFscan:PS51489	PANTHER:PTHR14030	PANTHER:PTHR14030:SF14	UniProt:Q2QYB8	SMART:SM00777	UniParc:UPI0000681B1A
RefSeq:XP_015618840.1	SEG:seg	:	:	:	:

Description

Mitotic checkpoint serine/threonine protein kinase, Bub1 domain containing protein. (Os12t0125300-01)

Coordinates

chr12:-:1212877..1216296

Molecular Weight (calculated)

69747.1 Da

IEP (calculated)

6.914

GRAVY (calculated)

-0.807

Length

622 amino acids

Sequence
(BLAST)

001: MAAATEQHCG GGGGDKEKDL LSAVVGDIRS YSGSDPLRPW LRGMRKMEAA LPPATLRAKL PRFLQKCAQE FQDDARYRDD SRYLRVWIQL MDYVKDAKPL
101: LKKMEKNRIG LKRSAFYMAY AVYYEKHKRF EDAENMYRLG TQNLAEPVGE LQKAHEQFIR RVELYKRRKS RVQQERMPNK VQSIAISKNE VEGQSRSCTK
201: PKSNPVQRSG SGSNPHLGFP HPLGRPLSRG TSGETMSLSR HNSDDTVVVR FVGSALVGKS ETEDACHHGL VEPTINTKEA MDAISSMFLE PLEPETKLKR
301: RSNRDKPSFN QEASAFEIFV DEDEPNKSGP SKLQDKNMKQ DNPKLSQQAS AFEIFVDEDD PYCNNQNMVQ HRHFNKENTQ VNQNASGFEI FVDENEAHGN
401: GRNAMSHKSS GCPPKPSRDS KQQANFDFQK PFVGGFAILP DDEDEQLEKN DNGVKINSGT VQLTDDKDTS LCSRQTDSKI RCDDLRPAIS GLREDTVFHR
501: FVGSAVVGEP KVENACHHGL VEPTVNLKEA MDDINNMFGI PLNFKGDKPK NKKTTALSER KAALLSGFSI LADDEPGENP AAQVKPSNAS KFECQSGLFE
601: PTITTRDVMA EINDMFGMPL DF

Best Arabidopsis Sequence Match ( AT5G05510.1 )
(BLAST)

001: MAKNENGYEN LLASLIVDIK SYSGKDHLLP WIRGVKKMKE SLPSQILNEK LPRFLQKCAE SFESDKRYKN DSRYIRVWLQ LMDFVDDPRA LLRTMEAKSI
101: GTKRSLFYQA YALHYEKMKR FEDAEKMYRL GVQNLAEPMD ELQKSYLQFV TRMERHKKKK TQRQEQKLSG KHHKVDERQQ HEPVLNFVDK AIVGKPEAEN
201: ACHHGLVDPT INMKEAMNTI NNMFKEPIET APLQRRSRQR SQNKENQGCN NSFEVFLDEN LECETGTSGK AKTSTTQCGS QPNQESFEIF IDDENTDETA
301: DENDEAGKAF VFLLPRDHSP ESSEEADRNT PPRARFREDT VVRRFVGSTI SDEPEAVENA CHHGLVDPTV NLKEAMEDIN NMFGEPINFV RPNRSKNKGK
401: AVVETKPNPA AGFSILEDDD EEEAEQEHQG KSQPTQRLPS KSDERELFEP TVCTKVALDE INKLFAMPMD F

Arabidopsis Description

At5g05510 [Source:UniProtKB/TrEMBL;Acc:Q9FFG4]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.