Factsheet for Zm00001d044390_P001

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

extracellular 6
endoplasmic reticulum 4
vacuole 5
plasma membrane 4
golgi 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	secretory
iPSORT:	secretory
MultiLoc:	extracellular
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	extracellular
YLoc:	vacuole

mitochondrion: 27297264
plasma membrane: 27341663

msms PMID: 27297264 doi

D Dahal, KJ Newton, BP Mooney

Division of Biological Sciences, ‡Department of Biochemistry, and §The Charles W Gehrke Proteomics Center, University of Missouri , Columbia, Missouri 65211, United States.

msms PMID: 27341663 doi

P Voothuluru, JC Anderson, RE Sharp, SC Peck

Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA., Division of Biochemistry, University of Missouri, Columbia, MO, 65211, USA., Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA., Interdisciplinary Plant Group, University of Missouri, Columbia, MO, 65211, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
Zm00001d015521_P001

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G01300.1	Zm00001d015521_P001	AT4G31920.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES01017	Sorghum	mitochondrion, plastid	89.28	83.59
TraesCS3A01G214100.1	Wheat	nucleus	79.79	79.3
TraesCS3D01G216700.1	Wheat	vacuole	79.79	79.14
Os01t0598600-01	Rice	nucleus	80.0	77.6
HORVU3Hr1G052320.1	Barley	mitochondrion, plasma membrane, plastid, vacuole	80.41	77.38
TraesCS3B01G244800.1	Wheat	plastid	80.62	73.91
Solyc01g096040.2.1	Tomato	nucleus, plastid	55.26	55.72
PGSC0003DMT400000651	Potato	vacuole	55.05	55.62
VIT_15s0046g00360.t01	Wine grape	extracellular	54.85	54.4
KRH47900	Soybean	nucleus	52.78	54.12
CDY15187	Canola	vacuole	53.61	54.05
KRG97194	Soybean	endoplasmic reticulum	52.58	54.03
KRH68920	Soybean	nucleus	51.34	54.01
CDX90023	Canola	vacuole	53.4	53.85
Bra033271.1-P	Field mustard	vacuole	53.4	53.85
KRH06457	Soybean	endoplasmic reticulum, vacuole	52.78	53.78
AT1G01300.1	Thale cress	vacuole	53.2	53.2
CDY43952	Canola	vacuole	52.37	52.59
Bra014420.1-P	Field mustard	vacuole	52.37	52.59
Solyc10g009410.1.1	Tomato	extracellular	49.28	52.18
AT3G61820.1	Thale cress	extracellular, vacuole	51.96	52.17
CDX89133	Canola	vacuole	51.96	52.17
CDX67868	Canola	vacuole	51.96	51.96
Bra003476.1-P	Field mustard	vacuole	51.55	51.55
PGSC0003DMT400048585	Potato	vacuole	48.45	50.98
GSMUA_Achr3P31640_001	Banana	cytosol	58.97	50.89
CDY25081	Canola	vacuole	50.72	50.72
Zm00001d039910_P001	Maize	vacuole	47.22	45.26
Zm00001d043617_P001	Maize	endoplasmic reticulum, extracellular, vacuole	41.44	44.47
Zm00001d045852_P001	Maize	plastid	13.4	41.94
Zm00001d001835_P001	Maize	vacuole	39.59	41.38
Zm00001d045855_P001	Maize	vacuole	29.9	39.94
Zm00001d001833_P001	Maize	plastid	36.49	39.6
Zm00001d037411_P001	Maize	plastid	31.96	37.71
Zm00001d020887_P001	Maize	vacuole	34.43	35.61
Zm00001d045831_P001	Maize	plastid	34.02	34.23
Zm00001d045836_P001	Maize	extracellular	32.78	33.76
Zm00001d022078_P001	Maize	vacuole	35.26	33.4
Zm00001d006330_P001	Maize	vacuole	35.05	33.33
Zm00001d017867_P001	Maize	golgi, vacuole	32.58	33.05
Zm00001d051692_P001	Maize	endoplasmic reticulum, plasma membrane	31.75	32.77
Zm00001d020886_P001	Maize	endoplasmic reticulum, vacuole	32.16	32.7
Zm00001d017869_P001	Maize	plasma membrane	31.34	32.69
Zm00001d017866_P002	Maize	extracellular	31.34	32.55
Zm00001d045863_P001	Maize	vacuole	29.48	32.13
Zm00001d045843_P001	Maize	endoplasmic reticulum, extracellular, vacuole	30.52	31.76
Zm00001d045849_P001	Maize	vacuole	30.31	31.34
Zm00001d017864_P001	Maize	plastid	32.58	30.5
Zm00001d051693_P001	Maize	endoplasmic reticulum, vacuole	28.45	30.13
Zm00001d045842_P001	Maize	endoplasmic reticulum, plasma membrane, vacuole	28.45	29.87
Zm00001d020536_P001	Maize	extracellular	30.1	29.2
Zm00001d051695_P001	Maize	mitochondrion	31.55	28.81
Zm00001d037409_P001	Maize	vacuole	28.04	28.57
Zm00001d035981_P001	Maize	extracellular	28.04	28.27
Zm00001d040433_P001	Maize	cytosol	20.62	28.01
Zm00001d050178_P001	Maize	plasma membrane	30.31	25.13
Zm00001d036827_P002	Maize	plasma membrane, vacuole	33.2	15.54

Protein Annotations

EntrezGene:100501808	MapMan:19.4.3.1	Gene3D:2.40.70.10	UniProt:A0A1D6NLC5	InterPro:Aspartic_peptidase_A1	InterPro:Aspartic_peptidase_AS
InterPro:CND41-like	GO:GO:0003674	GO:GO:0003824	GO:GO:0004190	GO:GO:0006508	GO:GO:0008150
GO:GO:0008152	GO:GO:0008233	GO:GO:0016787	GO:GO:0019538	InterPro:IPR021109	InterPro:IPR033121
ProteinID:ONM41026.1	InterPro:PEPTIDASE_A1	PFAM:PF14541	PFAM:PF14543	PRINTS:PR00792	ScanProsite:PS00141
PFscan:PS51767	PANTHER:PTHR13683	PANTHER:PTHR13683:SF355	InterPro:Peptidase_aspartic_dom_sf	SUPFAM:SSF50630	SignalP:SignalP-noTM
InterPro:TAXi_C	InterPro:TAXi_N	UniParc:UPI00022132CC	EnsemblPlantsGene:Zm00001d044390	EnsemblPlants:Zm00001d044390_P001	EnsemblPlants:Zm00001d044390_T001
SEG:seg	:	:	:	:	:

Description

Aspartyl protease family protein 2

Coordinates

chr3:-:226837569..226839600

Molecular Weight (calculated)

49934.2 Da

IEP (calculated)

8.530

GRAVY (calculated)

0.095

Length

485 amino acids

Sequence
(BLAST)

001: MTTLAALLLL VAASNVVKAV EYHSFVATPL SPHLYTAPSL DADEDVFGGS LAVAEEAAAA SDSAVHFRVV HRDTFAVNAT AGELLKHRLQ RDKRRAARIS
101: EAAGAGGGNG RKGVAAPVVS GLAQGSGEYF TKIGVGTPAT QALMVLDTGS DVVWVQCAPC RRCYEQSGPV FDPRRSSSYG AVGCGAALCR RLDSGGCDLR
201: RGACMYQVAY GDGSVTAGDF VTETLTFAGG ARVARVALGC GHDNEGLFVA AAGLLGLGRG GLSFPTQISR RYGRSFSYCL VDRTSSGAGA APGSHRSSTV
301: SFGAGSVGAS SASFTPMVRN PRMETFYYVQ LVGISVGGAR VPGVAESDLR LDPSTGRGGV IVDSGTSVTR LARASYSALR DAFRAAAAGG LRLSPGGFSL
401: FDTCYDLGGR RVVKVPTVSM HFAGGAEAAL PPENYLIPVD SRGTFCFAFA GTDGGVSIIG NIQQQGFRVV FDGDGQRVGF APKGC

Best Arabidopsis Sequence Match ( AT1G01300.1 )
(BLAST)

001: MVGRRKALLF SLCFFFLSLP SFSSLPSFQT LFPNSHSLPC ASPVSFQPDS DSESLLESEF ESGSDSESSS SITLNLDHID ALSSNKTPDE LFSSRLQRDS
101: RRVKSIATLA AQIPGRNVTH APRPGGFSSS VVSGLSQGSG EYFTRLGVGT PARYVYMVLD TGSDIVWLQC APCRRCYSQS DPIFDPRKSK TYATIPCSSP
201: HCRRLDSAGC NTRRKTCLYQ VSYGDGSFTV GDFSTETLTF RRNRVKGVAL GCGHDNEGLF VGAAGLLGLG KGKLSFPGQT GHRFNQKFSY CLVDRSASSK
301: PSSVVFGNAA VSRIARFTPL LSNPKLDTFY YVGLLGISVG GTRVPGVTAS LFKLDQIGNG GVIIDSGTSV TRLIRPAYIA MRDAFRVGAK TLKRAPDFSL
401: FDTCFDLSNM NEVKVPTVVL HFRGADVSLP ATNYLIPVDT NGKFCFAFAG TMGGLSIIGN IQQQGFRVVY DLASSRVGFA PGGCA

Arabidopsis Description

APF2Aspartyl protease family protein 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9LNJ3]
SUBAcon: [vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.