Factsheet for PGSC0003DMT400048585

Subcellular Localization

min:

: max

Winner_takes_all: vacuole

Predictor Summary:

extracellular 6
golgi 5
plasma membrane 6
endoplasmic reticulum 5
vacuole 7
peroxisome 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	vacuole
Any Predictor:	peroxisome, secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	vacuole
Plant-mPloc:	plasma membrane
Predotar:	secretory
PProwler:	secretory
PTS1:	peroxisome
TargetP:	secretory
WoLF PSORT:	extracellular
YLoc:	vacuole

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400008290
PGSC0003DMT400020233

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G01300.1	PGSC0003DMT400008290	AT4G31920.1	18642946
AT1G01300.1	PGSC0003DMT400020233	AT4G31920.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc10g009410.1.1	Tomato	extracellular	95.66	96.29
PGSC0003DMT400000651	Potato	vacuole	74.4	71.46
KRG97194	Soybean	endoplasmic reticulum	65.94	64.41
KRH47900	Soybean	nucleus	65.51	63.85
KRH06457	Soybean	endoplasmic reticulum, vacuole	65.29	63.24
KRH68920	Soybean	nucleus	62.91	62.91
VIT_15s0046g00360.t01	Wine grape	extracellular	66.38	62.58
CDX90023	Canola	vacuole	65.08	62.37
Bra033271.1-P	Field mustard	vacuole	64.86	62.16
AT1G01300.1	Thale cress	vacuole	65.08	61.86
CDY15187	Canola	vacuole	64.43	61.75
AT3G61820.1	Thale cress	extracellular, vacuole	63.12	60.25
CDX89133	Canola	vacuole	61.82	59.01
Bra014420.1-P	Field mustard	vacuole	61.61	58.8
CDX67868	Canola	vacuole	61.39	58.35
CDY43952	Canola	vacuole	60.74	57.97
Bra003476.1-P	Field mustard	vacuole	60.95	57.94
CDY25081	Canola	vacuole	60.09	57.11
TraesCS3A01G214100.1	Wheat	nucleus	52.71	49.8
TraesCS3D01G216700.1	Wheat	vacuole	52.71	49.69
Os01t0598600-01	Rice	nucleus	53.58	49.4
HORVU3Hr1G052320.1	Barley	mitochondrion, plasma membrane, plastid, vacuole	53.36	48.81
Zm00001d044390_P001	Maize	plasma membrane	50.98	48.45
Os05t0590000-01	Rice	extracellular	49.89	47.82
TraesCS3A01G287800.1	Wheat	cytosol	36.01	46.5
EES01017	Sorghum	mitochondrion, plastid	51.84	46.14
TraesCS3B01G244800.1	Wheat	plastid	52.93	46.12
KXG22664	Sorghum	extracellular, plastid, vacuole	49.46	45.51
Os01t0720000-00	Rice	vacuole	43.38	44.84
GSMUA_Achr3P31640_001	Banana	cytosol	54.23	44.48
PGSC0003DMT400033734	Potato	vacuole	46.42	44.4
PGSC0003DMT400014352	Potato	endoplasmic reticulum, vacuole	45.55	43.21
Zm00001d043617_P001	Maize	endoplasmic reticulum, extracellular, vacuole	41.87	42.7
HORVU3Hr1G068330.1	Barley	plastid, vacuole	40.13	41.95
OQU92922	Sorghum	vacuole	44.47	41.84
KXG24090	Sorghum	extracellular	44.69	41.62
KXG33265	Sorghum	vacuole	41.0	41.54
KXG24092	Sorghum	vacuole	44.03	41.26
PGSC0003DMT400005606	Potato	nucleus	41.65	41.2
TraesCS3D01G287600.1	Wheat	vacuole	39.91	41.16
PGSC0003DMT400010420	Potato	endoplasmic reticulum, plastid	43.6	40.94
EES16428	Sorghum	extracellular	43.82	40.89
PGSC0003DMT400065088	Potato	extracellular	42.3	40.71
TraesCS3B01G322500.1	Wheat	vacuole	38.83	40.41
OQU79707	Sorghum	vacuole	42.52	38.97
PGSC0003DMT400095799	Potato	vacuole	36.23	31.16
PGSC0003DMT400059566	Potato	extracellular	32.75	31.13
PGSC0003DMT400091831	Potato	vacuole	35.79	30.16
PGSC0003DMT400036706	Potato	vacuole	30.59	29.75
PGSC0003DMT400074390	Potato	vacuole	29.72	29.21

Protein Annotations

EntrezGene:102601674	MapMan:19.4.3.1	Gene3D:2.40.70.10	MEROPS:A01.050	InterPro:Aspartic_peptidase_A1	InterPro:Aspartic_peptidase_AS
InterPro:CND41-like	GO:GO:0003674	GO:GO:0003824	GO:GO:0004190	GO:GO:0006508	GO:GO:0008150
GO:GO:0008152	GO:GO:0008233	GO:GO:0016787	GO:GO:0019538	InterPro:IPR021109	InterPro:IPR033121
UniProt:M1BMM5	InterPro:PEPTIDASE_A1	PFAM:PF14541	PFAM:PF14543	EnsemblPlantsGene:PGSC0003DMG400018877	PGSC:PGSC0003DMG400018877
EnsemblPlants:PGSC0003DMT400048585	PRINTS:PR00792	ScanProsite:PS00141	PFscan:PS51767	PANTHER:PTHR13683	PANTHER:PTHR13683:SF308
InterPro:Peptidase_aspartic_dom_sf	SUPFAM:SSF50630	InterPro:TAXi_C	InterPro:TAXi_N	TMHMM:TMhelix	UniParc:UPI000295E209
RefSeq:XP_006362137.1	SEG:seg	:	:	:	:

Description

Aspartic proteinase nepenthesin-1 [Source:PGSC_GENE;Acc:PGSC0003DMG400018877]

Coordinates

chr10:+:5258629..5260282

Molecular Weight (calculated)

49592.0 Da

IEP (calculated)

8.392

GRAVY (calculated)

0.016

Length

461 amino acids

Sequence
(BLAST)

001: MEVKLISILF FFTIFVAAYS LQYQTLTLNS LPQLQSLSWE PQLSSDNENT LSVQLHHVDL LSPSSFNATP HALFKLRLQR DAIRAKSLSL LSASAANSTG
101: KDFSSSVISG LSQGSGEYFT RIGIGTPTKY VYLVLDTGSD IVWIQCLPCA KCYSQSDPVF DPSKSSSFSP VTCDSPLCRR LDSSGCNNRN KCLYQVSYGD
201: GSFTVGEYST ETLNFRKTTV SNISFGCGHD NEGLFIGAAG LLGLGKGKLS FPGQAGSRFG QKFSYCLADR IGSAKPSYIV FGESAITRNT IFTPLLTNPK
301: LDTFYYVELT GISVGGTKVP AVTPELFKLD TEGNGGVIVD SGTSVTRLTR PGYIAVRDAF RLGAKDLKRA PNFSLFDTCY DLSGKTQAKV PTLVLHFAGA
401: DVALPAANYM IPVNGEGRYC LGFAGTNGGL SIIGNIQQQG FRVVFDLAGS RLGFAPRGCS Y

Best Arabidopsis Sequence Match ( AT1G01300.1 )
(BLAST)

001: MVGRRKALLF SLCFFFLSLP SFSSLPSFQT LFPNSHSLPC ASPVSFQPDS DSESLLESEF ESGSDSESSS SITLNLDHID ALSSNKTPDE LFSSRLQRDS
101: RRVKSIATLA AQIPGRNVTH APRPGGFSSS VVSGLSQGSG EYFTRLGVGT PARYVYMVLD TGSDIVWLQC APCRRCYSQS DPIFDPRKSK TYATIPCSSP
201: HCRRLDSAGC NTRRKTCLYQ VSYGDGSFTV GDFSTETLTF RRNRVKGVAL GCGHDNEGLF VGAAGLLGLG KGKLSFPGQT GHRFNQKFSY CLVDRSASSK
301: PSSVVFGNAA VSRIARFTPL LSNPKLDTFY YVGLLGISVG GTRVPGVTAS LFKLDQIGNG GVIIDSGTSV TRLIRPAYIA MRDAFRVGAK TLKRAPDFSL
401: FDTCFDLSNM NEVKVPTVVL HFRGADVSLP ATNYLIPVDT NGKFCFAFAG TMGGLSIIGN IQQQGFRVVY DLASSRVGFA PGGCA

Arabidopsis Description

APF2Aspartyl protease family protein 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9LNJ3]
SUBAcon: [vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.