CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

extracellular 5
endoplasmic reticulum 5
vacuole 7
plasma membrane 6
golgi 5
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	plastid, secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	vacuole
Plant-mPloc:	plasma membrane
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	vacuole

nucleus: 21132161
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH68920	Soybean	nucleus	87.92	90.02
KRH47900	Soybean	nucleus	77.54	77.38
KRH06457	Soybean	endoplasmic reticulum, vacuole	77.12	76.47
Bra033271.1-P	Field mustard	vacuole	73.09	71.73
CDX90023	Canola	vacuole	73.09	71.73
CDY15187	Canola	vacuole	72.88	71.52
AT1G01300.1	Thale cress	vacuole	72.67	70.72
VIT_15s0046g00360.t01	Wine grape	extracellular	72.88	70.35
Solyc01g096040.2.1	Tomato	nucleus, plastid	71.61	70.27
PGSC0003DMT400000651	Potato	vacuole	71.4	70.21
Solyc10g009410.1.1	Tomato	extracellular	64.83	66.81
PGSC0003DMT400048585	Potato	vacuole	64.41	65.94
TraesCS3D01G216700.1	Wheat	vacuole	56.99	55.01
TraesCS3A01G214100.1	Wheat	nucleus	56.78	54.92
Os01t0598600-01	Rice	nucleus	57.2	54.0
HORVU3Hr1G052320.1	Barley	mitochondrion, plasma membrane, plastid, vacuole	57.2	53.57
Os05t0590000-01	Rice	extracellular	53.81	52.81
Zm00001d044390_P001	Maize	plasma membrane	54.03	52.58
TraesCS3B01G244800.1	Wheat	plastid	56.14	50.09
EES01017	Sorghum	mitochondrion, plastid	54.66	49.81
GSMUA_Achr3P31640_001	Banana	cytosol	58.9	49.47
KXG22664	Sorghum	extracellular, plastid, vacuole	52.33	49.3
TraesCS3A01G287800.1	Wheat	cytosol	35.59	47.06
KRH70570	Soybean	plastid	47.46	46.28
KRH75429	Soybean	plasma membrane, plastid, vacuole	47.25	46.07
KRH39233	Soybean	nucleus	47.46	45.53
KRG90588	Soybean	extracellular, vacuole	44.7	44.61
Os01t0720000-00	Rice	vacuole	41.95	44.39
KRH40917	Soybean	extracellular, vacuole	38.98	44.02
OQU92922	Sorghum	vacuole	44.92	43.27
KXG24090	Sorghum	extracellular	45.13	43.03
EES16428	Sorghum	extracellular	44.92	42.91
KRH36168	Soybean	endoplasmic reticulum, extracellular, vacuole	43.01	42.74
Zm00001d043617_P001	Maize	endoplasmic reticulum, extracellular, vacuole	40.89	42.7
HORVU3Hr1G068330.1	Barley	plastid, vacuole	39.19	41.95
KXG24092	Sorghum	vacuole	43.64	41.87
KXG33265	Sorghum	vacuole	40.25	41.76
TraesCS3D01G287600.1	Wheat	vacuole	39.19	41.39
OQU79707	Sorghum	vacuole	43.64	40.95
TraesCS3B01G322500.1	Wheat	vacuole	38.14	40.63
KRH46246	Soybean	cytosol	25.85	39.23
KRH46242	Soybean	vacuole	36.02	35.05
KRH46241	Soybean	nucleus, vacuole	35.81	34.63
KRH11684	Soybean	vacuole	34.53	34.53
KRG98688	Soybean	nucleus	35.59	34.29
KRH46245	Soybean	nucleus	34.53	33.82
KRH36688	Soybean	mitochondrion, vacuole	33.9	32.99
KRH73276	Soybean	cytosol	27.97	32.27
KRH09627	Soybean	vacuole	30.72	30.85
KRH47308	Soybean	extracellular	31.14	30.56
KRH73277	Soybean	extracellular	29.45	30.15
KRH44589	Soybean	extracellular, vacuole	30.51	29.88
KRG98962	Soybean	vacuole	35.38	29.82
KRH46018	Soybean	vacuole	34.96	29.62
KRH73176	Soybean	extracellular, vacuole	28.6	29.22
KRG98063	Soybean	vacuole	33.05	29.1
KRH30714	Soybean	vacuole	32.63	28.36
KRH73625	Soybean	vacuole	33.26	27.99
KRH57273	Soybean	endoplasmic reticulum, golgi	16.1	26.57
KRH14503	Soybean	vacuole	34.32	22.16

Protein Annotations

EntrezGene:100794269	MapMan:19.4.3.1	Gene3D:2.40.70.10	MEROPS:A01.A05	EMBL:ACUP02012179	InterPro:Aspartic_peptidase_A1
InterPro:Aspartic_peptidase_AS	InterPro:CND41-like	EnsemblPlantsGene:GLYMA_19G256400	GO:GO:0003674	GO:GO:0003824	GO:GO:0004190
GO:GO:0006508	GO:GO:0008150	GO:GO:0008152	GO:GO:0008233	GO:GO:0009056	GO:GO:0016787
GO:GO:0019538	GO:GO:0030163	UniProt:I1NCM4	InterPro:IPR021109	InterPro:IPR033121	EnsemblPlants:KRG97194
ProteinID:KRG97194	ProteinID:KRG97194.1	InterPro:PEPTIDASE_A1	PFAM:PF14541	PFAM:PF14543	ScanProsite:PS00141
PFscan:PS51767	PANTHER:PTHR13683	PANTHER:PTHR13683:SF308	InterPro:Peptidase_aspartic_dom_sf	SUPFAM:SSF50630	SignalP:SignalP-noTM
InterPro:TAXi_C	InterPro:TAXi_N	TMHMM:TMhelix	UniParc:UPI0001CAA8DD	SEG:seg	:

Description

hypothetical protein

Coordinates

chr19:-:50040646..50042808

Molecular Weight (calculated)

50914.9 Da

IEP (calculated)

8.919

GRAVY (calculated)

-0.015

Length

472 amino acids

Sequence
(BLAST)

001: MEGKKKTTNC LLFLFLSLTL SLSLSAALQL QTQTLPLHSL PHPPAISWPE SESEPDPEEE ALSLHLHHID ALSSNKTPEQ LFQLRLQRDA KRVEGVVALA
101: ALNQSHARRS GSSFSSSIIS GLAQGSGEYF TRIGVGTPAR YVYMVLDTGS DVVWLQCAPC RKCYTQADPV FDPTKSRTYA GIPCGAPLCR RLDSPGCNNK
201: NKVCQYQVSY GDGSFTFGDF STETLTFRRT RVTRVALGCG HDNEGLFIGA AGLLGLGRGR LSFPVQTGRR FNQKFSYCLV DRSASAKPSS VVFGDSAVSR
301: TARFTPLIKN PKLDTFYYLE LLGISVGGSP VRGLSASLFR LDAAGNGGVI IDSGTSVTRL TRPAYIALRD AFRVGASHLK RAAEFSLFDT CFDLSGLTEV
401: KVPTVVLHFR GADVSLPATN YLIPVDNSGS FCFAFAGTMS GLSIIGNIQQ QGFRVSFDLA GSRVGFAPRG CV

Best Arabidopsis Sequence Match ( AT1G01300.1 )
(BLAST)

001: MVGRRKALLF SLCFFFLSLP SFSSLPSFQT LFPNSHSLPC ASPVSFQPDS DSESLLESEF ESGSDSESSS SITLNLDHID ALSSNKTPDE LFSSRLQRDS
101: RRVKSIATLA AQIPGRNVTH APRPGGFSSS VVSGLSQGSG EYFTRLGVGT PARYVYMVLD TGSDIVWLQC APCRRCYSQS DPIFDPRKSK TYATIPCSSP
201: HCRRLDSAGC NTRRKTCLYQ VSYGDGSFTV GDFSTETLTF RRNRVKGVAL GCGHDNEGLF VGAAGLLGLG KGKLSFPGQT GHRFNQKFSY CLVDRSASSK
301: PSSVVFGNAA VSRIARFTPL LSNPKLDTFY YVGLLGISVG GTRVPGVTAS LFKLDQIGNG GVIIDSGTSV TRLIRPAYIA MRDAFRVGAK TLKRAPDFSL
401: FDTCFDLSNM NEVKVPTVVL HFRGADVSLP ATNYLIPVDT NGKFCFAFAG TMGGLSIIGN IQQQGFRVVY DLASSRVGFA PGGCA

Arabidopsis Description

APF2Aspartyl protease family protein 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9LNJ3]
SUBAcon: [vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.