Factsheet for Bra035529.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

plastid 9
mitochondrion 2
cytosol 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra027469.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G35630.1	Bra027469.1-P	AT5G42980.1	15352244

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Bra035392.1-P	Field mustard	plastid	97.66	97.66
CDX84116	Canola	plastid	56.07	97.17
AT5G35630.1	Thale cress	plastid	92.29	91.86
GSMUA_Achr9P17480_001	Banana	cytosol	72.2	86.8
Solyc01g080280.2.1	Tomato	plastid	85.75	84.95
PGSC0003DMT400011133	Potato	cytosol	85.51	84.72
VIT_05s0020g02480.t01	Wine grape	plastid	82.94	82.18
VIT_07s0104g00610.t01	Wine grape	plastid	82.94	81.99
GSMUA_Achr3P09120_001	Banana	mitochondrion	76.4	80.94
Bra039756.1-P	Field mustard	cytosol	65.65	78.93
Os04t0659100-01	Rice	plastid	78.27	78.27
Bra008612.1-P	Field mustard	cytosol	64.72	77.81
TraesCS2B01G528300.1	Wheat	plastid	76.64	77.54
TraesCS2A01G500400.1	Wheat	plastid	77.34	77.52
TraesCS2D01G500600.1	Wheat	plastid	77.34	77.52
Bra023573.1-P	Field mustard	cytosol	64.25	77.25
Bra028132.1-P	Field mustard	cytosol	63.78	76.69
KXG27313	Sorghum	plastid	77.57	76.67
Bra022247.1-P	Field mustard	cytosol	62.38	75.42
Bra021276.1-P	Field mustard	cytosol	61.92	74.86
Bra018729.1-P	Field mustard	cytosol	59.35	72.16
Bra001686.1-P	Field mustard	endoplasmic reticulum, golgi	57.71	69.77
Bra032257.1-P	Field mustard	cytosol	23.13	58.24
KRH25519	Soybean	plastid	22.66	51.05

Protein Annotations

KEGG:00220+6.3.1.2	KEGG:00250+6.3.1.2	KEGG:00630+6.3.1.2	KEGG:00910+6.3.1.2	MapMan:25.1.5.1.2	Gene3D:3.10.20.70
Gene3D:3.30.590.40	EnsemblPlantsGene:Bra035529	EnsemblPlants:Bra035529.1	EnsemblPlants:Bra035529.1-P	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0004356	GO:GO:0005488	GO:GO:0005524	GO:GO:0006542	GO:GO:0006807
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016874	InterPro:Gln_synt_N
InterPro:Gln_synt_b-grasp	InterPro:Gln_synth/guanido_kin_cat_dom	InterPro:Gln_synth_N_conserv_site	InterPro:Gln_synth_cat_dom	InterPro:Gln_synth_gly_rich_site	InterPro:IPR036651
UniProt:M4F379	PFAM:PF00120	PFAM:PF03951	ScanProsite:PS00180	ScanProsite:PS00181	PANTHER:PTHR20852
PANTHER:PTHR20852:SF53	SMART:SM01230	SUPFAM:SSF54368	SUPFAM:SSF55931	UniParc:UPI0002546382	:

Description

AT5G35630 (E=2e-238) GS2, GLN2, ATGSL1 | GS2 (GLUTAMINE SYNTHETASE 2); glutamate-ammonia ligase

Coordinates

chrA08:-:8242679..8244889

Molecular Weight (calculated)

47346.2 Da

IEP (calculated)

6.277

GRAVY (calculated)

-0.446

Length

428 amino acids

Sequence
(BLAST)

001: MAQILAASPT CQMKLTKPSP IASSKLWSSV MLKQKKQNSS KLRGFKVMAL QSDNSTINRV ESLLNLDTKP FTDRIIAEYI WIGGSGIDLR SKSRTLEKPV
101: EDPSELPKWN YDGSSTGQAP GEDSEVILYP QAIFRDPFRG GDNILVICDT YTPAGEPIPT NKRARAAEIF SNKKVNEEIP WFGIEQEYTL LQPNVNWPLG
201: WPVGAYPGPQ GPYYCGVGAE KSWGRDISDA HYKACLYAGI NISGTNGEVM PGQWEFQVGP SVGIEAGDHV WCARYLLERI TEQAGVVLTL DPKPIEGDWN
301: GAGCHTNYST KSMREEGGFD VIKKAILNLS LRHMEHISAY GEGNERRLTG KHETASIDQF SWGVANRGCS IRVGRDTEKK GKGYLEDRRP ASNMDPYIVT
401: SLLAETTLLW EPTLEAEALA AQKLSLKV

Best Arabidopsis Sequence Match ( AT5G35630.3 )
(BLAST)

001: MAQILAASPT CQMRVPKHSS VIASSSKLWS SVVLKQKKQS NNKVRGFRVL ALQSDNSTVN RVETLLNLDT KPYSDRIIAE YIWIGGSGID LRSKSRTIEK
101: PVEDPSELPK WNYDGSSTGQ APGEDSEVIL YPQAIFRDPF RGGNNILVIC DTWTPAGEPI PTNKRAKAAE IFSNKKVSGE VPWFGIEQEY TLLQQNVKWP
201: LGWPVGAFPG PQGPYYCGVG ADKIWGRDIS DAHYKACLYA GINISGTNGE VMPGQWEFQV GPSVGIDAGD HVWCARYLLE RITEQAGVVL TLDPKPIEGD
301: WNGAGCHTNY STKSMREEGG FEVIKKAILN LSLRHKEHIS AYGEGNERRL TGKHETASID QFSWGVANRG CSIRVGRDTE AKGKGYLEDR RPASNMDPYI
401: VTSLLAETTL LWEPTLEAEA LAAQKLSLNV

Arabidopsis Description

GLN2Glutamine synthetase, chloroplastic/mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q43127]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.