CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, golgi

Predictor Summary:

nucleus 2
cytosol 1
extracellular 2
endoplasmic reticulum 3
vacuole 2
plasma membrane 2
golgi 3
mitochondrion 2
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum, golgi
Any Predictor:	cytosol, mitochondrion, nucleus, plastid, secretory
BaCelLo:	nucleus
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	mitochondrion
WoLF PSORT:	plastid
YLoc:	golgi

PPI

Inferred distinct locusB in Crop

locusB	locations
EER91974
EER95112
KXG36538
KXG39799

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G63900.1	KXG39799	AT1G02280.1	23118188
AT1G63900.1	EER91974	AT3G16620.1	23118188
AT1G63900.1	EER95112	AT3G46740.1	23118188
AT1G63900.1	KXG36538	AT3G46740.1	23118188

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS2D01G141100.1	Wheat	endoplasmic reticulum, golgi	91.55	92.08
TraesCS2B01G162000.1	Wheat	endoplasmic reticulum, golgi	91.55	92.08
HORVU2Hr1G024550.6	Barley	endoplasmic reticulum, golgi	91.55	92.08
TraesCS2A01G138100.2	Wheat	endoplasmic reticulum, golgi	91.25	91.79
Os07t0647800-01	Rice	endoplasmic reticulum	75.51	90.24
GSMUA_Achr7P18450_001	Banana	endoplasmic reticulum, golgi	79.01	79.01
VIT_07s0104g00630.t01	Wine grape	plasma membrane, vacuole	74.64	74.64
KRH71471	Soybean	endoplasmic reticulum, golgi	72.01	72.86
KRH31972	Soybean	endoplasmic reticulum, golgi	71.72	72.57
PGSC0003DMT400051847	Potato	endoplasmic reticulum, golgi	71.72	71.93
CDY25541	Canola	endoplasmic reticulum, vacuole	70.55	70.97
AT1G63900.1	Thale cress	endoplasmic reticulum, golgi	70.85	70.85
CDY34384	Canola	endoplasmic reticulum, vacuole	70.55	70.55
Bra027667.1-P	Field mustard	endoplasmic reticulum, golgi	70.55	70.55
Zm00001d007060_P003	Maize	extracellular, plasma membrane	83.09	70.2
Solyc06g084360.2.1	Tomato	nucleus	71.14	64.04
Bra040424.1-P	Field mustard	endoplasmic reticulum, extracellular	51.31	50.57
CDX89169	Canola	extracellular	51.6	50.57
CDX93939	Canola	extracellular	51.31	50.0
AT1G59560.1	Thale cress	endoplasmic reticulum, extracellular, golgi	46.65	47.34
Bra007725.1-P	Field mustard	plasma membrane	47.23	42.41
CDY63334	Canola	endoplasmic reticulum, extracellular	47.52	41.9

Protein Annotations

MapMan:19.2.2.1.5.3.2	MapMan:19.2.5.1	Gene3D:3.30.40.10	EntrezGene:8072220	UniProt:C5X430	EnsemblPlants:EER99814
ProteinID:EER99814	ProteinID:EER99814.1	InterPro:GIDE	GO:GO:0003674	GO:GO:0003824	GO:GO:0004842
GO:GO:0006464	GO:GO:0006996	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016043
GO:GO:0016567	GO:GO:0016740	GO:GO:0019538	InterPro:IPR001841	InterPro:IPR013083	PFAM:PF12483
PFAM:PF13920	PFscan:PS50089	PANTHER:PTHR12183	PANTHER:PTHR12183:SF18	EnsemblPlantsGene:SORBI_3002G399100	SUPFAM:SSF57850
unigene:Sbi.2625	SignalP:SignalP-noTM	UniParc:UPI0001A83DA1	RefSeq:XP_002463293.1	InterPro:Znf_RING	InterPro:Znf_RING/FYVE/PHD
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr2:-:75015809..75021567

Molecular Weight (calculated)

37584.8 Da

IEP (calculated)

8.923

GRAVY (calculated)

-0.014

Length

343 amino acids

Sequence
(BLAST)

001: MMIPWGGVGC CLSAAALYLL GRSSGRDAEV LRSVARAGSM KDLAAILDTA SKVLPLVVAV SGRVSSDTPL ICQQSGMRGV IVEETAEQHF LKHNDAGSWI
101: QDSAVMLSVS KEVPWYLDDG TGRVYVVGAR SAAGLILTVA SEVFEESGRT LVRGTLDYLQ GLKMLGVKRT ERVLPTGTSL TVVGEAIRDD VGTIRIQRPH
201: KGPFYVSPKS IDQLIMNLGK WAKLYRLASM GFATFGVFLL AKRAIQHFLE RKRRHELQKR VLNAAAQRQA REAEGSNGSS DTEPNSKKDQ LVLDICVICL
301: EQEYNAVFVP CGHMCCCMAC SSHLTNCPLC RRRIDQAVRT FRH

Best Arabidopsis Sequence Match ( AT1G63900.1 )
(BLAST)

001: MIPWGGVTCC LSAAALYLLG RSSGRDAEVL ETVTRVNQLK ELAQLLELDS KILPFIVAVS GRVGSETPIK CEHSGIRGVI VEETAEQHFL KHNETGSWVQ
101: DSALMLSMSK EVPWFLDDGT SRVHVMGARG ATGFALTVGS EVFEESGRSL VRGTLDYLQG LKMLGVKRIE RVLPTGIPLT IVGEAVKDDI GEFRIQKPDR
201: GPFYVSSKSL DQLISNLGKW SRLYKYASMG FTVLGVFLIT KHVIDSVLER RRRRQLQKRV LDAAAKRAEL ESEGSNGTRE SISDSTKKED AVPDLCVICL
301: EQEYNAVFVP CGHMCCCTAC SSHLTSCPLC RRRIDLAVKT YRH

Arabidopsis Description

SP1E3 ubiquitin-protein ligase SP1 [Source:UniProtKB/Swiss-Prot;Acc:Q8L7N4]
SUBAcon: [endoplasmic reticulum,golgi]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.