CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol, nucleus

Predictor Summary:

nucleus 3
plastid 1
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG88461
KRH08941
KRH09220
KRH11760
KRH30256
KRH30262
KRH36762
KRH38460
KRH38714
KRH54731
KRH63183
KRH65727
KRH70353
KRH75969

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G23880.1	KRH11760	AT1G30460.1	16897494
AT5G23880.1	KRH36762	AT1G30460.1	16897494
AT5G23880.1	KRH11760	AT1G30460.1	18479511
AT5G23880.1	KRH36762	AT1G30460.1	18479511
AT5G23880.1	KRH70353	AT2G01730.1	16897494
AT5G23880.1	KRH70353	AT2G01730.1	18479511
AT5G23880.1	KRG88461	AT5G01400.1	17008405
AT5G23880.1	KRH65727	AT5G01400.1	17008405
AT5G23880.1	KRH75969	AT5G01400.1	17008405
AT5G23880.1	KRH08941	AT5G23880.1	16897494
AT5G23880.1	KRH30256	AT5G23880.1	16897494
AT5G23880.1	KRH30262	AT5G23880.1	16897494
AT5G23880.1	KRH38460	AT5G23880.1	16897494
AT5G23880.1	KRH09220	AT5G51660.1	16897494
AT5G23880.1	KRH38714	AT5G51660.1	16897494
AT5G23880.1	KRH09220	AT5G51660.1	17008405
AT5G23880.1	KRH38714	AT5G51660.1	17008405
AT5G23880.1	KRH09220	AT5G51660.1	18479511
AT5G23880.1	KRH38714	AT5G51660.1	18479511
AT5G23880.1	KRH54731	AT5G60940.1	18479511
AT5G23880.1	KRH63183	AT5G60940.1	18479511

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH38460	Soybean	cytosol	97.56	97.43
KRH30256	Soybean	cytosol	86.45	94.38
VIT_16s0050g00590.t01	Wine grape	cytosol	83.6	83.38
Solyc03g025540.2.1	Tomato	nucleus	79.67	79.57
AT5G23880.1	Thale cress	cytosol	79.27	79.16
KRH30262	Soybean	cytosol	59.49	78.82
CDX80392	Canola	cytosol	77.64	77.54
Bra009712.1-P	Field mustard	cytosol	77.51	77.4
CDX88053	Canola	cytosol	77.24	77.13
GSMUA_Achr1P15280_001	Banana	cytosol	76.56	76.87
Os09t0569400-01	Rice	cytosol	73.58	73.58
EER93127	Sorghum	cytosol	72.63	72.63
TraesCS5D01G464200.1	Wheat	cytosol	71.54	71.54
TraesCS5B01G462500.2	Wheat	cytosol	71.41	71.31
TraesCS5A01G453700.5	Wheat	cytosol	71.14	71.04
Zm00001d044552_P006	Maize	cytosol, plasma membrane	72.09	69.45
HORVU5Hr1G109220.1	Barley	plastid	72.09	67.51
KRH70353	Soybean	cytosol	14.77	20.49
KRH44086	Soybean	cytosol	16.94	18.17
KRH26351	Soybean	nucleus	16.8	18.0

Protein Annotations

EntrezGene:100796817	MapMan:16.2.1.2.2	Gene3D:3.60.15.10	EMBL:ACUP02010216	InterPro:Beta_Casp	InterPro:CPSF2
InterPro:CPSF2_MBL	ncoils:Coil	InterPro:Cpsf2_C	EnsemblPlantsGene:GLYMA_16G182300	GO:GO:0003674	GO:GO:0003676
GO:GO:0003723	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005654	GO:GO:0005847	GO:GO:0006139	GO:GO:0006378	GO:GO:0006379	GO:GO:0006397
GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	InterPro:IPR036866	UniProt:K7MI53	EnsemblPlants:KRH08941
ProteinID:KRH08941	ProteinID:KRH08941.1	InterPro:Metallo-B-lactamas	PFAM:PF07521	PFAM:PF10996	PFAM:PF13299
PFAM:PF16661	PANTHER:PTHR11203	PANTHER:PTHR11203:SF5	InterPro:RMMBL	InterPro:RibonucZ/Hydroxyglut_hydro	SMART:SM01027
SUPFAM:SSF56281	UniParc:UPI000233AD75	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr16:+:34307278..34322761

Molecular Weight (calculated)

81883.7 Da

IEP (calculated)

4.892

GRAVY (calculated)

-0.142

Length

738 amino acids

Sequence
(BLAST)

001: MGTSVQVTPL CGVYNENPLS YLVSIDGFNF LVDCGWNDHF DPSLLQPLAR VASTIDAVLL SHADTLHLGA LPYAMKQLGL SAPVYSTEPV YRLGLLTMYD
101: QYLSRKQVSE FDLFTLDDID SSFQSVTRLT YSQNHHFSGK GEGIVIAPHV AGHLLGGTIW KITKDGEDVI YAVDFNHRKE RHLNGTVLGS FVRPAVLITD
201: AYNALNNQPY RRQNDKEFGD ILKKTLREGG NVLLPVDTVG RVLELILMLE SYWTDENLNY PIYFLTYVAS STIDYVKSFL EWMSDTIAKS FEKTRENIFL
301: LKYVTLLINK TELDNAPDGP KVVLASMASL EAGFSHEIFV EWANDVKNLV LFTERGQFAT LARMLQADPP PKAVKVVVSK RVALVGEELI AYEEEQNRIK
401: KEALKASLMK EEEFKTSHGA DNNTSDSMVI DSGNNHVPPE VSGPRGGGYR DIFIDGFVPP LTSVAPMFPC YENTSEWDDF GEVINPDDYV IKDEDMDQTA
501: MHGGDINGKL DEGAASLILD TKPSKVVSDE RTVQVRCSLV YMDFEGRSDG RSIKNILSHV APLKLVLVHG SAEATEHLKQ HCLKHVCPHV YAPQLEETID
601: VTSDLCAYKV LLSEKLMSNV LFKKLGDYEL AWVDAVVGKT ENDPLSLLPV SGAAPPHKSV LVGDLKLADI KQFLSSKGVQ VEFAGGALRC GEYVTLRKVG
701: DASQKGGGSG AQQIVIEGPL CEDYYKIRDY LYSQFYLL

Best Arabidopsis Sequence Match ( AT5G23880.1 )
(BLAST)

001: MGTSVQVTPL CGVYNENPLS YLVSIDGFNF LIDCGWNDLF DTSLLEPLSR VASTIDAVLL SHPDTLHIGA LPYAMKQLGL SAPVYATEPV HRLGLLTMYD
101: QFLSRKQVSD FDLFTLDDID SAFQNVIRLT YSQNYHLSGK GEGIVIAPHV AGHMLGGSIW RITKDGEDVI YAVDYNHRKE RHLNGTVLQS FVRPAVLITD
201: AYHALYTNQT ARQQRDKEFL DTISKHLEVG GNVLLPVDTA GRVLELLLIL EQHWSQRGFS FPIYFLTYVS SSTIDYVKSF LEWMSDSISK SFETSRDNAF
301: LLRHVTLLIN KTDLDNAPPG PKVVLASMAS LEAGFAREIF VEWANDPRNL VLFTETGQFG TLARMLQSAP PPKFVKVTMS KRVPLAGEEL IAYEEEQNRL
401: KREEALRASL VKEEETKASH GSDDNSSEPM IIDTKTTHDV IGSHGPAYKD ILIDGFVPPS SSVAPMFPYY DNTSEWDDFG EIINPDDYVI KDEDMDRGAM
501: HNGGDVDGRL DEATASLMLD TRPSKVMSNE LIVTVSCSLV KMDYEGRSDG RSIKSMIAHV SPLKLVLVHA IAEATEHLKQ HCLNNICPHV YAPQIEETVD
601: VTSDLCAYKV QLSEKLMSNV IFKKLGDSEV AWVDSEVGKT ERDMRSLLPM PGAASPHKPV LVGDLKIADF KQFLSSKGVQ VEFAGGGALR CGEYVTLRKV
701: GPTGQKGGAS GPQQILIEGP LCEDYYKIRD YLYSQFYLL

Arabidopsis Description

CPSF100Cleavage and polyadenylation specificity factor subunit 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9LKF9]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.