CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

nucleus 3
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91748
KRH08941
KRH11760
KRH30256
KRH30262
KRH35025
KRH36762
KRH38460

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G60940.1	KRH11760	AT1G30460.1	18479511
AT5G60940.1	KRH36762	AT1G30460.1	18479511
AT5G60940.1	KRG91748	AT1G71800.1	18479511
AT5G60940.1	KRH35025	AT1G71800.1	18479511
AT5G60940.1	KRH08941	AT5G23880.1	18479511
AT5G60940.1	KRH30256	AT5G23880.1	18479511
AT5G60940.1	KRH30262	AT5G23880.1	18479511
AT5G60940.1	KRH38460	AT5G23880.1	18479511

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH63183	Soybean	cytosol	68.24	90.34
GSMUA_Achr6P35010_001	Banana	cytosol	61.18	77.84
PGSC0003DMT400059810	Potato	cytosol	29.18	75.61
Solyc12g038540.1.1	Tomato	cytosol	11.53	75.38
VIT_17s0000g05080.t01	Wine grape	cytosol	76.71	67.36
AT5G60940.1	Thale cress	cytosol	67.76	67.13
KXG37531	Sorghum	cytosol	66.59	66.9
TraesCS5D01G402200.1	Wheat	cytosol	65.41	66.67
Os03t0754900-02	Rice	cytosol	67.76	66.36
TraesCS5A01G392400.1	Wheat	cytosol	65.65	65.8
CDY02182	Canola	cytosol	64.71	65.63
TraesCS5B01G397200.1	Wheat	cytosol	65.41	65.26
HORVU5Hr1G095960.3	Barley	cytosol	64.94	65.25
CDY58694	Canola	cytosol	66.35	65.13
Zm00001d034054_P002	Maize	cytosol	65.88	62.64
Bra012994.1-P	Field mustard	cytosol	64.71	61.52

Protein Annotations

EntrezGene:100809908	MapMan:16.2.1.3.3	Gene3D:2.130.10.10	EMBL:ACUP02003574	InterPro:G-protein_beta_WD-40_rep	EnsemblPlantsGene:GLYMA_06G205000
GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	UniProt:I1KD31	InterPro:IPR001680	InterPro:IPR015943
InterPro:IPR017986	EnsemblPlants:KRH54731	ProteinID:KRH54731	ProteinID:KRH54731.1	PFAM:PF00400	PRINTS:PR00320
ScanProsite:PS00678	PFscan:PS50082	PFscan:PS50294	PANTHER:PTHR44133	PANTHER:PTHR44133:SF1	SMART:SM00320
SUPFAM:SSF50978	UniParc:UPI0001BA871A	InterPro:WD40/YVTN_repeat-like_dom_sf	InterPro:WD40_repeat	InterPro:WD40_repeat_CS	InterPro:WD40_repeat_dom
InterPro:WD40_repeat_dom_sf	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr6:-:19370319..19375364

Molecular Weight (calculated)

46798.4 Da

IEP (calculated)

7.647

GRAVY (calculated)

-0.246

Length

425 amino acids

Sequence
(BLAST)

001: MDNSSSEQTV QDGKLYRNLN SLIVAHLRDN SLTQAASAVA SATMTPLNVE APPNRLLDLV AKGLAVEKGD ITRGTGFTAT LPLPHTTAID FSSVPDVKGS
101: SKSFPKHETR HLSEHKNIAR CARFSADGRF VATGSADTSI KLFEVSKIKQ MLLPEAKDGP VRPVIRTYYD HIQPINDLDF HPQGTILISG AKDQTIKFFD
201: ISKTNAKRAY RVIQDTHNVR SVSFHPSGDF LLAGTDHAIP HLYDINTFQC YLSANIPETS PSGAINQIRY SCTGSMYVTA SKDGAIRLWD GITANCVRSI
301: TAAHGTAEAT SAIFTKDQRF VLSCGKDSTL KLWEVGSGRL VKQYLGAIHT QLRCQAIFNE TEEFILSIDE LSNEIVIWDA MTTEKVAKWP SNHVGAPRWL
401: EHSPIESAFI SCGTDRSVRF WKETV

Best Arabidopsis Sequence Match ( AT5G60940.1 )
(BLAST)

001: MGNSGDLEQA LQDGNIFRQL NALIVAHLRH HNLSQVASAV ASATMTPLNI EVPPNRLLEL VAKGLAAENN GTLRGVSSSV LLPSSYGSIT TPRTASIDFS
101: VNHAKGSSKT IPKHESKTLS EHKSVVRCAR FSPDGMFFAT GGADTSIKLF EVPKVKQMIS GDTQARPLIR TFYDHAEPIN DLDFHPRSTI LISSAKDNCI
201: KFFDFSKTTA KRAFKVFQDT HNVRSISFHP SGEFLLAGTD HPIPHLYDVN TYQCFLPSNF PDSGVSGAIN QVRYSSTGSI YITASKDGAI RLFDGVSAKC
301: VRSIGNAHGK SEVTSAVFTK DQRFVLSSGK DSTVKLWEIG SGRMVKEYLG AKRVKLRSQA IFNDTEEFVI SIDEASNEVV TWDARTADKV AKWPSNHNGA
401: PRWIEHSPVE SVFVTCGIDR SIRFWKESV

Arabidopsis Description

CSTF50Cleavage stimulation factor subunit 50 [Source:UniProtKB/Swiss-Prot;Acc:Q8L4J2]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.