CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: extracellular, endoplasmic reticulum, vacuole, mitochondrion, plastid, plasma membrane, golgi

Predictor Summary:

extracellular 5
endoplasmic reticulum 5
vacuole 5
plasma membrane 5
golgi 5
plastid 3
mitochondrion 3

Predictors

GFP

MS/MS

Papers

Winner Takes All:	mitochondrion, plastid, secretory
Any Predictor:	mitochondrion, plastid, secretory
BaCelLo:	secretory
ChloroP:	plastid
EpiLoc:	plastid
iPSORT:	secretory
MultiLoc:	mitochondrion
Plant-mPloc:	mitochondrion
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	mitochondrion

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG98502
KRG98507
KRH46360
KRH46364

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G50460.1	KRG98502	AT4G26630.1	25387881
AT1G50460.1	KRG98507	AT4G26630.1	25387881
AT1G50460.1	KRH46360	AT4G26630.1	25387881
AT1G50460.1	KRH46364	AT4G26630.1	25387881

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH29072	Soybean	plastid	94.44	94.44
KRH27814	Soybean	endoplasmic reticulum, extracellular, golgi, plasma membrane, plastid, vacuole	84.33	85.34
KRH77671	Soybean	plastid	84.33	85.34
PGSC0003DMT400034299	Potato	endoplasmic reticulum	76.39	75.2
Solyc11g065220.1.1	Tomato	endoplasmic reticulum	76.19	75.15
VIT_06s0061g00040.t01	Wine grape	plastid	77.98	75.14
CDY37134	Canola	endoplasmic reticulum, extracellular	58.93	70.05
CDY22797	Canola	plastid	68.85	69.68
Os01t0940100-01	Rice	mitochondrion	49.21	69.47
Bra030490.1-P	Field mustard	plastid	68.45	69.28
AT1G50460.1	Thale cress	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, plastid, vacuole	68.45	69.28
CDY07303	Canola	plastid	68.25	69.08
Bra018850.1-P	Field mustard	plastid	68.25	69.08
CDY29586	Canola	cytosol	55.75	69.04
CDY03607	Canola	plastid	66.47	68.65
Bra014254.1-P	Field mustard	plastid	67.86	68.54
CDY68234	Canola	endoplasmic reticulum, extracellular, mitochondrion	56.55	68.35
CDY45062	Canola	plastid	66.07	68.24
Bra035821.1-P	Field mustard	plastid	67.26	67.13
GSMUA_Achr1P11390_001	Banana	plastid	67.06	67.06
AT3G20040.1	Thale cress	plastid	66.67	66.93
CDY24565	Canola	plastid	67.06	66.93
CDY37135	Canola	plastid	12.3	66.67
Zm00001d042146_P002	Maize	mitochondrion, plastid	65.67	66.6
CDY25373	Canola	plastid	66.67	66.53
GSMUA_AchrUn_...	Banana	cytosol	53.97	66.5
GSMUA_Achr11P...	Banana	endoplasmic reticulum	65.48	66.4
GSMUA_Achr8P29510_001	Banana	endoplasmic reticulum, mitochondrion	67.66	66.34
Zm00001d011889_P001	Maize	mitochondrion	64.88	65.79
CDY20557	Canola	extracellular, vacuole	16.47	63.85
TraesCS3A01G480900.1	Wheat	plastid	63.1	62.72
TraesCS3D01G475600.1	Wheat	plastid	62.9	62.65
TraesCS3B01G525400.2	Wheat	plastid	62.5	62.25
HORVU3Hr1G105380.4	Barley	cytosol	57.94	60.83
KRH74246	Soybean	plastid	56.35	57.26
KRH60208	Soybean	endoplasmic reticulum	56.55	57.23
KRH41490	Soybean	endoplasmic reticulum	56.35	57.03
KRH48988	Soybean	nucleus	56.15	56.83
Os05t0375100-00	Rice	mitochondrion	56.75	56.75
OQU77891	Sorghum	plastid	56.15	56.49
OQU88184	Sorghum	mitochondrion, plastid	65.67	56.29
KRH74248	Soybean	nucleus	54.76	55.65
HORVU1Hr1G057760.1	Barley	plastid	54.96	55.4
TraesCS1A01G234700.1	Wheat	mitochondrion, plastid	54.37	55.35
TraesCS1B01G248900.1	Wheat	mitochondrion, plastid	54.76	55.2
TraesCS1D01G237400.1	Wheat	plastid	54.37	54.8
KRH44270	Soybean	endoplasmic reticulum	53.17	54.47
Zm00001d037861_P001	Maize	plastid	53.97	54.08
KRH47202	Soybean	cytosol	40.28	50.12
KRH05943	Soybean	plastid	47.22	47.6
KRH17426	Soybean	plastid	46.43	46.71
KRH04729	Soybean	cytosol	11.11	35.9
KRH38576	Soybean	cytosol	20.63	35.14
KRH04349	Soybean	endoplasmic reticulum, mitochondrion	34.13	35.1
KRH58185	Soybean	endoplasmic reticulum, mitochondrion	33.93	34.9

Protein Annotations

KEGG:00010+2.7.1.1	KEGG:00051+2.7.1.1	KEGG:00052+2.7.1.1	KEGG:00500+2.7.1.1	KEGG:00520+2.7.1.1	KEGG:00521+2.7.1.1
KEGG:00524+2.7.1.1	EntrezGene:100790064	MapMan:3.1.4.3	Gene3D:3.30.420.40	Gene3D:3.40.367.20	EMBL:ACUP02007170
EnsemblPlantsGene:GLYMA_12G021700	GO:GO:0000166	GO:GO:0001678	GO:GO:0003674	GO:GO:0003824	GO:GO:0004340
GO:GO:0004396	GO:GO:0005488	GO:GO:0005524	GO:GO:0005536	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005829	GO:GO:0005975	GO:GO:0006091	GO:GO:0006096
GO:GO:0006139	GO:GO:0008150	GO:GO:0008152	GO:GO:0008865	GO:GO:0009056	GO:GO:0009058
GO:GO:0009987	GO:GO:0016301	GO:GO:0016310	GO:GO:0016740	GO:GO:0016773	GO:GO:0019158
GO:GO:0019725	GO:GO:0030246	GO:GO:0046835	GO:GO:0051156	InterPro:Hexokinase	InterPro:Hexokinase_BS
InterPro:Hexokinase_C	InterPro:Hexokinase_N	InterPro:IPR001312	UniProt:K7LSL4	EnsemblPlants:KRH24099	ProteinID:KRH24099
ProteinID:KRH24099.1	PFAM:PF00349	PFAM:PF03727	PRINTS:PR00475	ScanProsite:PS00378	PFscan:PS51257
PFscan:PS51748	PANTHER:PTHR19443	PANTHER:PTHR19443:SF6	SUPFAM:SSF53067	SignalP:SignalP-noTM	TMHMM:TMhelix
UniParc:UPI000233C5BC	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr12:+:1566157..1570771

Molecular Weight (calculated)

54801.5 Da

IEP (calculated)

6.795

GRAVY (calculated)

0.014

Length

504 amino acids

Sequence
(BLAST)

001: MGRVMVGVAV GLAAVACAVA AVAVGQRMRS RGNWKRVARV LKEVEEGCET SVGRLRQVVD AMAVEMHAGL ASEGGSKLKM LLTYVHNLPN GTEKGTYYAL
101: DLGGTNFRVL RVHLHGQQSS VLEHEVERQP IPQNLMTSTS EDLFDFIASS LKEFIEKEGD GSELSPDRRR ELGFTFSFPV KQMSVSSGIL LKWTKGFSIV
201: DLVGIDVPAC LQEALTRKGL DVRVAALVND TVGTLALGHY HDSDTVASVI IGTGTNACYL ERVDAIIKCQ GLPTTSGYMV VNMEWGNFWS SHLPRTSYDI
301: DLDSESPNPN DQGFEKMISG MYLGDIVRRV ILRMSLESDM LGPISSKLSM PFMLRTPMMA AMHEDDSPDL REVARILKGI LEIPDVPLKL RKVVVKVCDV
401: VTRRAARLAA AGIVGILKKI GRDGSGGIAG GRRSDMKMKR TVVAIEGGLY SSYTLFRDYL HEALNDILGE DIARHVILKV TEDGSGVGAA LLAASHSSYD
501: VDTV

Best Arabidopsis Sequence Match ( AT1G50460.1 )
(BLAST)

001: MGKVAVAFAA VAVVAACSVA AVMVGRRMKS RRKWRTVVEI LKELEDDCDT PVGRLRQVVD AMAVEMHAGL ASEGGSKLKM LLTFVDDLPT GREKGTYYAL
101: HLGGTYFRIL RVLLGDQRSY LDVQDVERHP IPSHLMNSTS EVLFNFLAFS LERFIEKEEN GSDSQGVRRE LAFTFSFPVK HTSISSGVLI KWTKGFEISE
201: MVGQDIAECL QGALNRRGLD MHVAALVNDT VGALSLGYYH DPDTVVAVVF GTGSNACYLE RTDAIIKCQG LLTTSGSMVV NMEWGNFWSS HLPRTSYDID
301: LDAESSNAND MGFEKMISGM YLGDIVRRVI LRMSEDSDIF GPISPVLSEP YVLRTNSVSA IHEDDTPELQ EVARILKDIG VSDVPLKVRK LVVKICDVVT
401: RRAGRLAAAG IAGILKKIGR DGSGGITSGR SRSEIQMQKR TVVAVEGGLY MNYTMFREYM EEALVEILGE EVSQYVVVKA MEDGSSIGSA LLVASLQS

Arabidopsis Description

HKL1Hexokinase-3 [Source:UniProtKB/Swiss-Prot;Acc:Q9LPS1]
SUBAcon: [extracellular,endoplasmic reticulum,vacuole,plastid,cytosol,plasma membrane,golgi]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.