CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: extracellular, endoplasmic reticulum, vacuole, plastid, plasma membrane, golgi

Predictor Summary:

cytosol 2
plastid 3
extracellular 4
endoplasmic reticulum 4
vacuole 4
plasma membrane 4
golgi 4
mitochondrion 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plastid, secretory
Any Predictor:	cytosol, mitochondrion, plastid, secretory
BaCelLo:	cytosol
ChloroP:	plastid
EpiLoc:	plastid
iPSORT:	secretory
MultiLoc:	mitochondrion
Plant-mPloc:	mitochondrion
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG98502
KRG98507
KRH46360
KRH46364

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G50460.1	KRG98502	AT4G26630.1	25387881
AT1G50460.1	KRG98507	AT4G26630.1	25387881
AT1G50460.1	KRH46360	AT4G26630.1	25387881
AT1G50460.1	KRH46364	AT4G26630.1	25387881

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH77671	Soybean	plastid	97.19	97.19
KRH24099	Soybean	endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	85.34	84.33
KRH29072	Soybean	plastid	84.94	83.93
VIT_06s0061g00040.t01	Wine grape	plastid	78.11	74.38
PGSC0003DMT400034299	Potato	endoplasmic reticulum	74.7	72.66
Solyc11g065220.1.1	Tomato	endoplasmic reticulum	74.3	72.41
CDY22797	Canola	plastid	71.49	71.49
AT1G50460.1	Thale cress	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, plastid, vacuole	71.29	71.29
CDY37134	Canola	endoplasmic reticulum, extracellular	60.44	70.99
CDY37135	Canola	plastid	13.25	70.97
Bra018850.1-P	Field mustard	plastid	70.68	70.68
CDY29586	Canola	cytosol	57.23	70.02
Bra014254.1-P	Field mustard	plastid	70.08	69.94
Bra030490.1-P	Field mustard	plastid	69.88	69.88
CDY45062	Canola	plastid	68.27	69.67
CDY03607	Canola	plastid	68.07	69.47
CDY07303	Canola	plastid	69.28	69.28
CDY68234	Canola	endoplasmic reticulum, extracellular, mitochondrion	57.83	69.06
Os01t0940100-01	Rice	mitochondrion	49.4	68.91
GSMUA_Achr1P11390_001	Banana	plastid	69.28	68.45
GSMUA_Achr8P29510_001	Banana	endoplasmic reticulum, mitochondrion	69.88	67.7
Bra035821.1-P	Field mustard	plastid	68.47	67.52
CDY24565	Canola	plastid	68.47	67.52
CDY25373	Canola	plastid	68.27	67.33
GSMUA_Achr11P...	Banana	endoplasmic reticulum	66.87	67.0
GSMUA_AchrUn_...	Banana	cytosol	54.62	66.5
AT3G20040.1	Thale cress	plastid	66.87	66.33
Zm00001d042146_P002	Maize	mitochondrion, plastid	65.86	66.0
Zm00001d011889_P001	Maize	mitochondrion	64.86	64.99
CDY20557	Canola	extracellular, vacuole	16.06	61.54
TraesCS3A01G480900.1	Wheat	plastid	62.45	61.34
TraesCS3B01G525400.2	Wheat	plastid	62.05	61.07
TraesCS3D01G475600.1	Wheat	plastid	61.45	60.47
HORVU3Hr1G105380.4	Barley	cytosol	57.83	60.0
KRH60208	Soybean	endoplasmic reticulum	57.43	57.43
Os05t0375100-00	Rice	mitochondrion	58.03	57.34
KRH41490	Soybean	endoplasmic reticulum	57.23	57.23
KRH48988	Soybean	nucleus	57.03	57.03
KRH74246	Soybean	plastid	56.43	56.65
OQU77891	Sorghum	plastid	56.43	56.09
HORVU1Hr1G057760.1	Barley	plastid	56.22	56.0
TraesCS1A01G234700.1	Wheat	mitochondrion, plastid	55.62	55.96
OQU88184	Sorghum	mitochondrion, plastid	65.86	55.78
TraesCS1B01G248900.1	Wheat	mitochondrion, plastid	55.62	55.4
TraesCS1D01G237400.1	Wheat	plastid	55.42	55.2
KRH74248	Soybean	nucleus	54.82	55.04
KRH44270	Soybean	endoplasmic reticulum	54.02	54.67
Zm00001d037861_P001	Maize	plastid	54.62	54.08
KRH47202	Soybean	cytosol	40.76	50.12
KRH05943	Soybean	plastid	48.19	48.0
KRH17426	Soybean	plastid	47.59	47.31
KRH04349	Soybean	endoplasmic reticulum, mitochondrion	36.55	37.14
KRH58185	Soybean	endoplasmic reticulum, mitochondrion	36.14	36.73
KRH04729	Soybean	cytosol	11.45	36.54
KRH38576	Soybean	cytosol	21.69	36.49

Protein Annotations

KEGG:00010+2.7.1.1	KEGG:00051+2.7.1.1	KEGG:00052+2.7.1.1	KEGG:00500+2.7.1.1	KEGG:00520+2.7.1.1	KEGG:00521+2.7.1.1
KEGG:00524+2.7.1.1	EntrezGene:100819464	MapMan:3.1.4.3	Gene3D:3.30.420.40	Gene3D:3.40.367.20	EMBL:ACUP02006734
EnsemblPlantsGene:GLYMA_11G015800	GO:GO:0000166	GO:GO:0001678	GO:GO:0003674	GO:GO:0003824	GO:GO:0004340
GO:GO:0004396	GO:GO:0005488	GO:GO:0005524	GO:GO:0005536	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005829	GO:GO:0005975	GO:GO:0006091	GO:GO:0006096
GO:GO:0006139	GO:GO:0008150	GO:GO:0008152	GO:GO:0008865	GO:GO:0009056	GO:GO:0009058
GO:GO:0009987	GO:GO:0016301	GO:GO:0016310	GO:GO:0016740	GO:GO:0016773	GO:GO:0019158
GO:GO:0019725	GO:GO:0030246	GO:GO:0046835	GO:GO:0051156	InterPro:Hexokinase	InterPro:Hexokinase_BS
InterPro:Hexokinase_C	InterPro:Hexokinase_N	UniProt:I1LG62	InterPro:IPR001312	EnsemblPlants:KRH27814	ProteinID:KRH27814
ProteinID:KRH27814.1	PFAM:PF00349	PFAM:PF03727	PRINTS:PR00475	ScanProsite:PS00378	PFscan:PS51748
PANTHER:PTHR19443	PANTHER:PTHR19443:SF6	SUPFAM:SSF53067	TMHMM:TMhelix	UniParc:UPI00023CE3BD	SEG:seg

Description

hypothetical protein

Coordinates

chr11:+:1104413..1109421

Molecular Weight (calculated)

54594.4 Da

IEP (calculated)

8.555

GRAVY (calculated)

-0.011

Length

498 amino acids

Sequence
(BLAST)

001: MGRVVVGVAV TVAVATCALA AVVVGRRVRS RRKWKKVANV VRELEEGCDT RVGRLRQVVD AMAVEMHAGL ASEGGSKLKM LLTFVDNLPN GTERGTYYAL
101: HLGGTNFRVL RVQLNGQPSS DFEHEVERQP IPQHVMTSTS EDLFDFIASS LKEFIAKEGD GSNISQDRRE LGFTFSFPVK QMSVSSGILI KWTKGFSIVD
201: MVGRDVAACL QEALTRKGLD MRVAALVNDT VGTLAVGHYH DPDTVAAIII GTGTNACYWE RVDAIIKCQG LLMTSGRMVV NMEWGNFWSS HLPRTSYDID
301: LDAESPNPND QGFEKMISGM YLGDIVRRVI LKMSLESDMF GPISPKLSMP FIMRTPLMSA MHEDNSPDLR EVARILNDIF EIPDIPLKAR KFVVKVCDVV
401: TRRAARLAAA GIVGILKKIG RDGSGGITGG RSRSDMKMRR TVVAIEGGLY SKYALFREYL REALNEILGE DIAKHVILKV TEDGSGIGAA LLAASYSS

Best Arabidopsis Sequence Match ( AT1G50460.1 )
(BLAST)

001: MGKVAVAFAA VAVVAACSVA AVMVGRRMKS RRKWRTVVEI LKELEDDCDT PVGRLRQVVD AMAVEMHAGL ASEGGSKLKM LLTFVDDLPT GREKGTYYAL
101: HLGGTYFRIL RVLLGDQRSY LDVQDVERHP IPSHLMNSTS EVLFNFLAFS LERFIEKEEN GSDSQGVRRE LAFTFSFPVK HTSISSGVLI KWTKGFEISE
201: MVGQDIAECL QGALNRRGLD MHVAALVNDT VGALSLGYYH DPDTVVAVVF GTGSNACYLE RTDAIIKCQG LLTTSGSMVV NMEWGNFWSS HLPRTSYDID
301: LDAESSNAND MGFEKMISGM YLGDIVRRVI LRMSEDSDIF GPISPVLSEP YVLRTNSVSA IHEDDTPELQ EVARILKDIG VSDVPLKVRK LVVKICDVVT
401: RRAGRLAAAG IAGILKKIGR DGSGGITSGR SRSEIQMQKR TVVAVEGGLY MNYTMFREYM EEALVEILGE EVSQYVVVKA MEDGSSIGSA LLVASLQS

Arabidopsis Description

HKL1Hexokinase-3 [Source:UniProtKB/Swiss-Prot;Acc:Q9LPS1]
SUBAcon: [extracellular,endoplasmic reticulum,vacuole,plastid,cytosol,plasma membrane,golgi]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.