CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

extracellular 4
endoplasmic reticulum 4
vacuole 4
plasma membrane 4
golgi 4
cytosol 3
mitochondrion 1
plastid 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, plastid, secretory
BaCelLo:	secretory
EpiLoc:	cytosol
iPSORT:	mitochondrion
MultiLoc:	cytosol
Plant-mPloc:	plastid
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH17367
KRH69472
KRH73005
KRH74682

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G44590.1	KRH69472	AT1G10510.1	21798944
AT3G44590.1	KRH74682	AT1G10510.1	21798944
AT3G44590.1	KRH17367	AT3G02885.1	21798944
AT3G44590.1	KRH73005	AT3G02885.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH67884	Soybean	endoplasmic reticulum	72.07	70.8
KRH32679	Soybean	endoplasmic reticulum	72.07	70.18
KRH30835	Soybean	endoplasmic reticulum	72.07	70.18
KRG96175	Soybean	extracellular	71.17	69.91
KRH22710	Soybean	endoplasmic reticulum	93.69	69.8
AT3G44590.1	Thale cress	extracellular	67.57	67.57
Bra000501.1-P	Field mustard	extracellular	68.47	66.67
CDX83277	Canola	extracellular	67.57	65.79
CDY31188	Canola	extracellular	67.57	65.79
AT2G27710.1	Thale cress	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	67.57	65.22
CDY04508	Canola	endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	65.77	64.6
TraesCS1B01G286500.1	Wheat	extracellular	64.86	64.29
Bra012004.1-P	Field mustard	endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	64.86	63.72
TraesCS1D01G276700.1	Wheat	extracellular	63.96	63.39
TraesCS1A01G277500.1	Wheat	nucleus	63.96	62.83
CDY30535	Canola	extracellular	63.96	62.83
Bra034363.1-P	Field mustard	extracellular	63.96	62.83
CDX71430	Canola	endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	63.96	62.28
HORVU1Hr1G073640.1	Barley	cytosol	63.06	61.95
CDX77131	Canola	extracellular	63.06	61.95
AT2G27720.2	Thale cress	extracellular	66.67	56.92
Os05t0445500-01	Rice	nucleus	63.06	50.36
KXG22113	Sorghum	endoplasmic reticulum, extracellular, plastid	61.26	46.9
Zm00001d010128_P003	Maize	cytosol	55.86	37.58

Protein Annotations

Gene3D:1.10.10.1410	MapMan:17.1.2.1.47	EMBL:ACUP02007724	EnsemblPlantsGene:GLYMA_12G182800	GO:GO:0002181	GO:GO:0003674
GO:GO:0003735	GO:GO:0005198	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005829	GO:GO:0005840	GO:GO:0006412	GO:GO:0006414	GO:GO:0008150	GO:GO:0008152
GO:GO:0009058	GO:GO:0009987	GO:GO:0019538	GO:GO:0022625	GO:GO:0030529	UniProt:I1LTT3
InterPro:IPR038716	EnsemblPlants:KRH26594	ProteinID:KRH26594	ProteinID:KRH26594.1	HAMAP:MF_01478	InterPro:P1/P2_N_sf
PFAM:PF00428	PANTHER:PTHR21141	PANTHER:PTHR21141:SF72	InterPro:Ribosomal_L12	UniParc:UPI00023D0AF5	SEG:seg

Description

hypothetical protein

Coordinates

chr12:-:34385415..34387011

Molecular Weight (calculated)

11174.1 Da

IEP (calculated)

4.015

GRAVY (calculated)

0.050

Length

111 amino acids

Sequence
(BLAST)

001: MKVVAAYLLA VLGGNPSPSA DDIKHILGAV GAEAENELIE LLLTEVKGKD FNELIASGSE KMSAVSGGGA AVAVAAAPAG GAAAPAAEAK EEKKVEEKEE
101: SDDDMGFSLF D

Best Arabidopsis Sequence Match ( AT3G44590.1 )
(BLAST)

001: MKVAAAFLLA VLGGNANPSA DNIKDIIGAV GADVDGESIE LLLKEVSGKD IAELIASGRE KLASVPSGGG VAVSAAPSSG GGGAAAPAEK KEAKKEEKEE
101: SDDDMGFSLF E

Arabidopsis Description

RPP2DAcidic ribosomal protein P2-like [Source:UniProtKB/TrEMBL;Acc:Q0WRF9]
SUBAcon: [extracellular]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.