CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 2
vacuole 3
cytosol 2
extracellular 3
endoplasmic reticulum 2
plasma membrane 2
golgi 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, nucleus, secretory
BaCelLo:	nucleus
EpiLoc:	vacuole
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	extracellular
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH23958
KRH39841

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G50680.1	KRH23958	AT4G25200.1	21798944
AT5G50680.1	KRH39841	AT4G25200.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH59806	Soybean	cytosol, nucleus	91.81	77.95
VIT_17s0000g01270.t01	Wine grape	cytosol	76.51	66.56
PGSC0003DMT400039493	Potato	cytosol	72.24	62.85
CDY08954	Canola	cytosol, mitochondrion	38.79	62.29
PGSC0003DMT400069346	Potato	cytosol	70.82	62.19
AT5G50580.2	Thale cress	cytosol	70.11	61.56
AT5G50680.1	Thale cress	cytosol	70.11	61.56
Solyc06g072080.2.1	Tomato	cytosol	68.68	60.69
CDY34327	Canola	cytosol	69.04	60.25
CDX93356	Canola	cytosol	68.68	59.94
CDX92842	Canola	cytosol	68.68	59.94
CDY03404	Canola	cytosol	68.68	59.94
Bra013848.1-P	Field mustard	cytosol	68.68	59.94
GSMUA_AchrUn_...	Banana	cytosol	68.68	59.75
AT4G24940.1	Thale cress	cytosol	67.97	59.32
Solyc03g019730.2.1	Tomato	mitochondrion	71.89	58.38
CDY08955	Canola	cytosol	20.64	54.72
Os11t0497000-01	Rice	cytosol	59.79	51.22
TraesCS7A01G352700.2	Wheat	cytosol	59.43	50.76
TraesCS7B01G275300.1	Wheat	cytosol	59.79	50.76
EES08513	Sorghum	cytosol	60.14	50.45
TraesCS7D01G370300.1	Wheat	cytosol	59.43	50.3
Zm00001d049163_P001	Maize	cytosol, extracellular	59.43	50.15
TraesCS3D01G090000.1	Wheat	cytosol	57.65	48.8
TraesCS3A01G090000.1	Wheat	cytosol	56.94	48.19
TraesCS3B01G105300.2	Wheat	cytosol	56.94	48.19
HORVU2Hr1G027820.1	Barley	cytosol	14.59	45.56
HORVU7Hr1G089410.1	Barley	cytosol, mitochondrion, nucleus	60.14	44.13
CDY08961	Canola	plastid	22.06	43.97
HORVU3Hr1G016090.4	Barley	peroxisome	35.59	39.53
HORVU3Hr1G016010.1	Barley	cytosol, plastid	56.94	34.33
KRH56132	Soybean	cytosol	22.42	12.05
KRH25373	Soybean	cytosol	21.71	11.66

Protein Annotations

EntrezGene:100809607	MapMan:19.2.2.2.2.1	Gene3D:3.40.50.720	EMBL:ACUP02004750	EnsemblPlantsGene:GLYMA_08G011400	GO:GO:0003674
GO:GO:0003824	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006464
GO:GO:0008150	GO:GO:0008152	GO:GO:0008641	GO:GO:0009987	GO:GO:0016925	GO:GO:0019538
GO:GO:0019948	UniProt:I1KP66	EnsemblPlants:KRH41120	ProteinID:KRH41120	ProteinID:KRH41120.1	PFAM:PF00899
PANTHER:PTHR10953	PANTHER:PTHR10953:SF162	SUPFAM:SSF69572	InterPro:ThiF_NAD_FAD-bd	UniParc:UPI00023C5AE2	InterPro:Ubiquitin-activating_enz

Description

hypothetical protein

Coordinates

chr8:-:903120..907093

Molecular Weight (calculated)

31138.5 Da

IEP (calculated)

5.288

GRAVY (calculated)

-0.072

Length

281 amino acids

Sequence
(BLAST)

001: MKGTVAEFCK NIVLAGVGSL TLVDDRVATE EVLSSNFLIP PDENAYSGKT LGELCCNSLK DFNPMVRVSI EKGDLSSFDV EFFSKFDVVV VSCCSLSAKK
101: LANDKCRKLS KRVAFYAVDC RDSCGEIFVD LQDYKYSKKK QDETVKCDLK YPSFEDALSV PWRALHRRMS KLYYAMRVIE KFEEAEGRSA GEVSIADLSG
201: VLKLKKEICT AQSLNESHVP DTLLERLVTN AIEFPPVCAI IGGILGQEVI KAISGKGDPL KNFFFFDAFD GKGIIEDLSP K

Best Arabidopsis Sequence Match ( AT5G50680.1 )
(BLAST)

001: MDGDELTEQE TALYDRQIRV WGAGAQRRLS KSHVLVSGIK GTVAEFCKNI VLAGVGSVTL LDDRLVTTEV FNANFLILPD ENAYVGKTVA EICCDSLKDF
101: NPMVHVSIEK GDLSTLGVDF FEKFDVVVIG YSSRATKKAV NEKCRNLAKD VAFYTVDCRG SCGEIFVDLQ NYKYTKKKLD ETVECELTFP SFEEAVSVPW
201: KPMPRRTAKL YFAMRVIELF EETEGRKPGE CSLSDLPRVL KLKKELCEGN SVSENHIPDI LLERLVSNNT EFPPACAIIG GILGQEVIKV ISGKGEPLKN
301: FFYFDAEDGK GVIEDLSHKL

Arabidopsis Description

SAE1B-2SUMO-activating enzyme subunit 1B-1 [Source:UniProtKB/Swiss-Prot;Acc:P0DI12]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.