CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plastid, nucleus

Predictor Summary:

plastid 11

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG98855
KRH37954
KRH46102

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G54810.1	KRG98855	AT5G54810.1	21798944
AT5G54810.1	KRH37954	AT5G54810.1	21798944
AT5G54810.1	KRH46102	AT5G54810.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG98855	Soybean	mitochondrion, plastid	92.9	94.48
KRH37954	Soybean	cytosol	30.27	94.16
PGSC0003DMT400037303	Potato	cytosol	77.04	90.0
GSMUA_Achr4P07140_001	Banana	mitochondrion	76.41	84.92
Solyc07g064280.2.1	Tomato	plastid	78.29	82.24
PGSC0003DMT400002722	Potato	plastid	78.08	82.02
VIT_19s0177g00310.t01	Wine grape	plastid	78.08	81.66
Solyc10g006400.2.1	Tomato	plastid	78.71	80.56
AT5G54810.1	Thale cress	plastid	76.62	78.09
AT4G27070.1	Thale cress	plastid	77.24	77.89
Zm00001d049610_P001	Maize	plastid	73.9	74.68
TraesCS7A01G308600.1	Wheat	plastid	73.28	74.68
TraesCS7D01G305300.1	Wheat	plastid	73.28	74.68
TraesCS7B01G208700.1	Wheat	plastid	73.07	74.47
EES13315	Sorghum	plastid	73.7	74.16
Os08t0135900-01	Rice	plastid	72.65	73.89
Zm00001d024702_P001	Maize	plastid	73.49	73.49
Bra026381.1-P	Field mustard	cytosol	15.24	72.28
CDX89281	Canola	cytosol	15.03	71.29
HORVU7Hr1G073460.3	Barley	plastid	73.7	69.9
KRH27603	Soybean	mitochondrion	50.1	51.95
KRG93806	Soybean	cytosol	14.82	29.71
KRH18295	Soybean	nucleus, plastid	24.22	23.11

Protein Annotations

KEGG:00260+4.2.1.20	KEGG:00400+4.2.1.20	EntrezGene:100792812	Gene3D:3.40.50.1100	MapMan:4.1.5.3.5.2	EMBL:ACUP02005318
EnsemblPlantsGene:GLYMA_08G312300	GO:GO:0000162	GO:GO:0003674	GO:GO:0003824	GO:GO:0004834	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006568	GO:GO:0008150	GO:GO:0008152
GO:GO:0008652	GO:GO:0009058	GO:GO:0009073	GO:GO:0009987	GO:GO:0016829	UniProt:I1JFS9
EnsemblPlants:KRH46102	ProteinID:KRH46102	ProteinID:KRH46102.1	HAMAP:MF_00133	PFAM:PF00291	PIRSF:PIRSF001413
InterPro:PLP-dep	ScanProsite:PS00168	PANTHER:PTHR42882	PANTHER:PTHR42882:SF3	SUPFAM:SSF53686	TIGRFAMs:TIGR00263
InterPro:Trp_synth_b_CS	InterPro:Trp_synth_beta	InterPro:Trp_synth_beta/beta-like	InterPro:Trypto_synt_PLP_dependent	UniParc:UPI000233702F	SEG:seg

Description

hypothetical protein

Coordinates

chr8:+:43204862..43209394

Molecular Weight (calculated)

52026.2 Da

IEP (calculated)

6.961

GRAVY (calculated)

-0.220

Length

479 amino acids

Sequence
(BLAST)

001: MAASIPFTGI NSSRVLPIPR EPHHPFSHLL SNMSKFASLR TPSSSSASSS SSSVSCSLTK DPYSVVPLEE HSKMDNGSVL LQRPDSFGRF GKFGGKYVPE
101: TLMHALTELE AAFYSLSADE EFQKELAGIL KDYVGRESPL YFAERLTEHY KRPNGEGPHI YLKREDLNHT GAHKINNAVA QALLAKRLGK KRIIAETGAG
201: QHGVATATVC ARFGLECIIY MGAQDMERQA LNVFRMRLLG AEVRPVHSGT ATLKDATSEA IRDWVTNVET THYILGSVAG PHPYPMMVRE FHAVIGKETR
301: KQALEKWGGK PDILIACVGG GSNAMGLFNE FVDDKDVRLI GVEAAGFGLD SGKHAATLTK GEVGVLHGAM SYLLQDDDGQ IVEPHSISAG LDYPGVGPEH
401: SFLKDLGRAE YHSITDEEAL EAFKRVSRLE GIIPALETSH ALAYLEKVCP TLPNGAKVVV NFSGRGDKDV QTAIKCLKL

Best Arabidopsis Sequence Match ( AT4G27070.1 )
(BLAST)

001: MATASTAATF RPSSVSASSE LTHLRSPSKL PKFTPLPSAR SRSSSSFSVS CTIAKDPAVV MADSEKIKAA GSDPTMWQRP DSFGRFGKFG GKYVPETLMH
101: ALSELETAFY SLATDEDFQR ELAEILKDYV GRESPLYFAE RLTEHYRREN GEGPLIYLKR EDLNHTGAHK INNAVAQALL AKRLGKKRII AETGAGQHGV
201: ATATVCARFG LQCIIYMGAQ DMERQALNVF RMRLLGAEVR GVHSGTATLK DATSEAIRDW VTNVETTHYI LGSVAGPHPY PMMVRDFHAV IGKETRKQAM
301: EKWGGKPDVL VACVGGGSNA MGLFHEFVDD TEVRMIGVEA AGFGLDSGKH AATLTKGDVG VLHGAMSYLL QDDDGQIIEP HSISAGLDYP GVGPEHSFLK
401: DVGRAEYFSV TDEEALEAFK RVSRLEGIIP ALETSHALAH LEKLCPTLPD GARVVLNFSG RGDKDVQTAI KYLEV

Arabidopsis Description

TSB2Tryptophan synthase [Source:UniProtKB/TrEMBL;Acc:Q0WS13]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.