CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plastid, nucleus

Predictor Summary:

plastid 10
cytosol 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus, plastid
Any Predictor:	cytosol, plastid
BaCelLo:	plastid
ChloroP:	plastid
EpiLoc:	cytosol
iPSORT:	plastid
MultiLoc:	plastid
Plant-mPloc:	plastid
Predotar:	plastid
PProwler:	plastid
TargetP:	plastid
WoLF PSORT:	plastid
YLoc:	plastid

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH22291
KRH27037

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G38530.1	KRH22291	AT2G42580.1	19000166
AT5G38530.1	KRH27037	AT2G42580.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG93806	Soybean	cytosol	40.64	85.36
Zm00001d046676_P001	Maize	extracellular	59.56	80.16
CDY63316	Canola	cytosol	61.35	79.79
VIT_14s0083g00460.t01	Wine grape	plastid	78.09	79.19
Solyc12g096190.1.1	Tomato	plastid	76.49	78.05
PGSC0003DMT400075555	Potato	plastid	75.3	76.83
CDX99679	Canola	cytosol	16.53	75.45
Bra028191.1-P	Field mustard	plastid	73.11	73.4
Bra028413.1-P	Field mustard	plastid	72.91	73.2
CDX74518	Canola	plastid	72.91	73.2
KXG20416	Sorghum	plastid	71.91	73.08
GSMUA_Achr2P05390_001	Banana	plastid	72.91	73.05
CDY01654	Canola	plastid	72.71	72.85
TraesCS7A01G392800.1	Wheat	plastid	70.52	72.69
HORVU7Hr1G092800.3	Barley	plastid	70.92	72.65
TraesCS7B01G294800.1	Wheat	mitochondrion, plastid	70.32	72.48
AT5G38530.1	Thale cress	plastid	72.51	71.94
CDY50576	Canola	plastid	68.33	71.91
CDY30314	Canola	plastid	68.33	71.91
CDY52465	Canola	plastid	71.51	71.8
CDX67409	Canola	plastid	68.13	71.7
TraesCS7D01G388400.3	Wheat	cytosol	70.32	71.6
CDX99680	Canola	plastid	70.72	71.0
Os06t0632200-02	Rice	plastid	68.33	70.43
Bra025430.1-P	Field mustard	plastid	69.12	70.24
TraesCS2A01G257700.1	Wheat	nucleus	10.56	51.46
KRH37954	Soybean	cytosol	8.57	27.92
KRG98855	Soybean	mitochondrion, plastid	23.31	24.84
KRH46102	Soybean	nucleus, plastid	23.11	24.22
KRH27603	Soybean	mitochondrion	19.32	21.0

Protein Annotations

KEGG:00260+4.2.1.20	KEGG:00400+4.2.1.20	EntrezGene:100802258	Gene3D:3.40.50.1100	MapMan:35.1	EMBL:ACUP02008055
EnsemblPlantsGene:GLYMA_13G049500	GO:GO:0000162	GO:GO:0003674	GO:GO:0003824	GO:GO:0004834	GO:GO:0005488
GO:GO:0006568	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0030170
UniProt:I1LWZ0	EnsemblPlants:KRH18295	ProteinID:KRH18295	ProteinID:KRH18295.1	HAMAP:MF_00133	PFAM:PF00291
InterPro:PLP-dep	ScanProsite:PS00168	PANTHER:PTHR43565	SUPFAM:SSF53686	TIGRFAMs:TIGR01415	InterPro:Trp_synth_b-like
InterPro:Trp_synth_b_CS	InterPro:Trp_synth_beta	InterPro:Trp_synth_beta/beta-like	InterPro:Trypto_synt_PLP_dependent	UniParc:UPI000233C2F2	SEG:seg

Description

hypothetical protein

Coordinates

chr13:-:14548296..14551750

Molecular Weight (calculated)

54811.3 Da

IEP (calculated)

7.529

GRAVY (calculated)

-0.131

Length

502 amino acids

Sequence
(BLAST)

001: MMAHCVFPVN ALPTPSPCFH SKVGKQWPQG FALSVRPTNP KRLSSACKVR ATLGASDKSI GIPNQWYNVI ADLPVKPPPP LHPKTYEPIK PDDLSPLFPD
101: ELIRQEIASD RFIDIPDEVL DVYKLWRPTP LIRAKRLEKL LDTPARIYYK YEGVSPAGSH KPNSAVPQAW YNLQAGVKNV VTETGAGQWG SALAFACSIF
201: GLGCEVWQVR ASYDSKPYRR LMMQTWGAKV HPSPSMITEA GRRMLQEDPS SPGSLGIAIS EAVEVAAKNA DTKYCLGSVL NHVLLHQSVI GEECIKQMEA
301: IGETPDVIIG CTGGGSNFAG LSFPFLREKL NKKINPVIRA VEPAACPSLT KGVYTYDYGD TAGMTPLMKM HTLGHDFVPD PIHAGGLRYH GMAPLISHVY
401: DLGLMEAIAI PQTECFQGAI QFARSEGLIP APEPTHAIAA TIREAIRCRE AGEAKVILTA MCGHGHFDLP AYEKYLQGNM VDLSFSEDKM KASLANIPQV
501: IT

Best Arabidopsis Sequence Match ( AT5G38530.1 )
(BLAST)

001: MASQLLLPPN QFTKSVSPQV FITGDCQGFS DLTLKRKSNQ ATRVSNGSSL RVKAALRSTH NKSVVEIPKQ WYNLVADLSV KPPPPLHPKT FEPIKPEDLA
101: HLFPNELIKQ EATQERFIDI PEEVLEIYKL WRPTPLIRAK RLEKLLQTPA RIYFKYEGGS PAGSHKPNTA VPQAYYNAKE GVKNVVTETG AGQWGSSLAF
201: ASSLFGLDCE VWQVANSYHT KPYRRLMMQT WGAKVHPSPS DLTEAGRRIL ESDPSSPGSL GIAISEAVEV AARNEDTKYC LGSVLNHVLL HQTIIGEECI
301: QQMENFGETP DLIIGCTGGG SNFAGLSFPF IREKLKGKIN PVIRAVEPSA CPSLTKGVYA YDFGDTAGLT PLMKMHTLGH DFIPDPIHAG GLRYHGMAPL
401: ISHVYEQGFM EAISIPQIEC FQGAIQFART EGIIPAPEPT HAIAATIREA LRCKETGEAK VILMAMCGHG HFDLTSYDKY LKGELVDLSF SEEKIRESLS
501: KVPHVV

Arabidopsis Description

TSBtype2Tryptophan synthase beta chain [Source:UniProtKB/TrEMBL;Acc:Q9FFW8]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.