CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 2
peroxisome 1
mitochondrion 3
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	mitochondrion, nucleus, peroxisome, secretory
BaCelLo:	nucleus
MultiLoc:	peroxisome
Plant-mPloc:	mitochondrion
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	golgi

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH01228
KRH18445
KRH44934

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G48530.1	KRH01228	AT3G29160.1	10417704
AT3G48530.1	KRH18445	AT3G29160.1	10417704
AT3G48530.1	KRH44934	AT3G29160.1	10417704

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH63760	Soybean	cytosol	89.68	95.13
KRH57227	Soybean	cytosol	76.15	76.15
KRH03956	Soybean	peroxisome	77.29	75.9
Solyc03g111310.2.1	Tomato	unclear	67.43	69.01
PGSC0003DMT400039410	Potato	peroxisome	67.43	69.01
Bra018050.1-P	Field mustard	cytosol	66.28	68.48
VIT_17s0000g01460.t01	Wine grape	peroxisome	67.43	68.37
CDY08080	Canola	cytosol	65.83	68.17
AT3G48530.1	Thale cress	cytosol	66.06	67.92
CDX68465	Canola	cytosol	65.83	67.69
CDY59154	Canola	cytosol	65.6	67.14
CDY70744	Canola	cytosol	57.34	66.31
Bra029902.1-P	Field mustard	mitochondrion	65.37	62.5
KXG26065	Sorghum	cytosol	58.72	60.52
Os04t0382300-01	Rice	cytoskeleton, cytosol, peroxisome	58.72	60.24
Zm00001d004006_P001	Maize	cytosol, extracellular	58.26	60.05
TraesCS2B01G299000.1	Wheat	cytosol	57.11	58.59
TraesCS2A01G281700.1	Wheat	cytosol	56.88	58.35
TraesCS2D01G280600.1	Wheat	cytosol	56.65	58.12
HORVU2Hr1G069060.5	Barley	cytosol	55.96	52.7
GSMUA_AchrUn_...	Banana	extracellular, vacuole	51.38	51.38
KRH74794	Soybean	cytosol	33.03	32.51
GSMUA_Achr3P19540_001	Banana	nucleus	59.63	30.2
KRH69332	Soybean	endoplasmic reticulum, vacuole	32.57	29.4
KRH60826	Soybean	nucleus	16.97	21.39
KRH51517	Soybean	nucleus	21.1	21.1

Protein Annotations

EMBL:ACUP02003508	EnsemblPlants:KRH54191	EnsemblPlantsGene:GLYMA_06G170700	EntrezGene:100776754	Gene3D:3.10.580.10	GO:GO:0005575
GO:GO:0016020	GO:GO:0016021	InterPro:CBS_dom	InterPro:IPR000644	PANTHER:PTHR13780	PANTHER:PTHR13780:SF36
PFAM:PF00571	PFscan:PS51371	ProteinID:KRH54191	ProteinID:KRH54191.1	SEG:seg	SMART:SM00116
SUPFAM:SSF54631	UniParc:UPI000233A8CD	UniProt:I1KC37	MapMan:18.4.5.1.3	MapMan:27.3.1.3	:

Description

hypothetical protein

Coordinates

chr6:-:14288629..14292939

Molecular Weight (calculated)

48560.5 Da

IEP (calculated)

5.347

GRAVY (calculated)

-0.102

Length

436 amino acids

Sequence
(BLAST)

001: MCKEKKRIII NLLALALQRE RERVIMVTME ESPRSPEAKL GLRVEDLWDI QEPQLSPDEK FNACFESIPV SAFPPPPSNQ EIEIKSDATL AEAVKILAEH
101: NILSAPVVDV DAPNDASWID RYIGIVEFAG IVVWILHQSE PTSPRSPSGG AANTAAVNGI TSEAFGLESA STTSGNFFED LTSSQLYKNT KVRDISGSFR
201: WAPFLALERS NSFLTMLLLL SKYKMKSIPV VDLGAGRIDN IITQSSVIHM LAECAGLQWF ESWGTKKLSE VGLPMVTPNH IIKVYEDEPV LQAFKLMRKK
301: RIGGLPVMDR GSSTAIGNIS LKDVQFLLTA PEIYHDYRSI TAKDFLTAIR SYLEKHEGAF PMSGDLITCN KDCTIKELIQ LLDHEKIHRV YVADNDGNLE
401: GLITLRDIIS RLVHEPRGYF GDFFDGVLPM PPNSRV

Best Arabidopsis Sequence Match ( AT3G48530.1 )
(BLAST)

001: MATVPEIKIM RSESLGHRSD VSSPEAKLGM RVEDLWDEQK PQLSPNEKLN ACFESIPVSA FPLSSDSQDI EIRSDTSLAE AVQTLSKFKV LSAPVVDVDA
101: PEDASWIDRY IGIVEFPGIV VWLLHQLEPP SPRSPAVAAS NGFSHDFTTD VLDNGDSAVT SGNFFEVLTS SELYKNTKVR DISGTFRWAP FLALQKENSF
201: LTMLLLLSKY KMKSIPVVDL GVAKIENIIT QSGVIHMLAE CAGLLWFEDW GIKTLSEVGL PIMSKDHIIK IYEDEPVLQA FKLMRRKRIG GIPVIERNSE
301: KPVGNISLRD VQFLLTAPEI YHDYRSITTK NFLVSVREHL EKCGDTSAPI MSGVIACTKN HTLKELILML DAEKIHRIYV VDDFGNLEGL ITLRDIIARL
401: VHEPSGYFGD FFDGVMPLPE NYRV

Arabidopsis Description

KING1SNF1-related protein kinase regulatory subunit gamma-1 [Source:UniProtKB/Swiss-Prot;Acc:Q8LBB2]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.