CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 2
mitochondrion 8

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
mitochondrion: 27780359
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG98502
KRG98507
KRH46360
KRH46364

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G20080.1	KRG98502	AT4G26630.1	25387881
AT5G20080.1	KRG98507	AT4G26630.1	25387881
AT5G20080.1	KRH46360	AT4G26630.1	25387881
AT5G20080.1	KRH46364	AT4G26630.1	25387881

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH03716	Soybean	mitochondrion	95.67	96.87
VIT_11s0016g04970.t01	Wine grape	mitochondrion	76.78	76.07
AT5G20080.1	Thale cress	mitochondrion	72.76	71.65
Solyc05g018520.2.1	Tomato	plastid	70.59	70.59
EES00217	Sorghum	mitochondrion	67.8	70.42
Zm00001d039656_P003	Maize	mitochondrion	67.49	70.1
TraesCS3D01G129500.1	Wheat	mitochondrion	66.87	69.9
Os01t0174300-01	Rice	mitochondrion	67.18	69.77
TraesCS3B01G147600.1	Wheat	mitochondrion, unclear	66.25	69.26
TraesCS3A01G128600.1	Wheat	mitochondrion	66.25	69.03
HORVU3Hr1G024170.3	Barley	mitochondrion	65.94	68.49
GSMUA_Achr10P...	Banana	mitochondrion	69.66	67.98
KRG89951	Soybean	mitochondrion	20.12	47.1
KRG94933	Soybean	mitochondrion	33.44	44.81
KRH64963	Soybean	endoplasmic reticulum	35.91	41.73
KRH13184	Soybean	endoplasmic reticulum, peroxisome, plastid	37.15	40.4
KRH44741	Soybean	endoplasmic reticulum, peroxisome, plastid	36.53	39.73
KRG89948	Soybean	endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	16.41	37.86
KRH18814	Soybean	cytosol, mitochondrion, plastid	23.22	16.38
KRH16606	Soybean	cytosol	35.29	13.06
KRH18816	Soybean	cytosol, mitochondrion, peroxisome	34.98	12.75
KRH16605	Soybean	cytosol	34.37	12.53
KRH53180	Soybean	cytosol, mitochondrion, peroxisome	33.75	12.25

Protein Annotations

Gene3D:2.40.30.10	Gene3D:3.40.50.80	MapMan:50.1.6	EMBL:ACUP02002788	InterPro:Fd_Rdtase_FAD-bd	InterPro:Flavoprot_Pyr_Nucl_cyt_Rdtase
EnsemblPlantsGene:GLYMA_05G008100	GO:GO:0003674	GO:GO:0003824	GO:GO:0004128	GO:GO:0008150	GO:GO:0008152
GO:GO:0016491	GO:GO:0055114	UniProt:I1K1F4	InterPro:IPR017927	EnsemblPlants:KRH56619	ProteinID:KRH56619
ProteinID:KRH56619.1	InterPro:NADH-Cyt_B5_reductase	InterPro:OxRdtase_FAD-bd_dom	InterPro:OxRdtase_FAD/NAD-bd	PFAM:PF00175	PFAM:PF00970
PRINTS:PR00371	PRINTS:PR00406	PFscan:PS51384	PANTHER:PTHR19370	PANTHER:PTHR19370:SF174	InterPro:Riboflavin_synthase-like_b-brl
SUPFAM:SSF52343	SUPFAM:SSF63380	UniParc:UPI0002339C9E	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr5:-:755273..760948

Molecular Weight (calculated)

35589.1 Da

IEP (calculated)

9.472

GRAVY (calculated)

-0.193

Length

323 amino acids

Sequence
(BLAST)

001: MAAFLRRFAR ATPILFAAQS NSSHTNLRLP FTAIAAISGG VSFLYYHSSP NFAHSQEAEQ VESKNIALVP DKWVEFKLQD TARVSHNTQL FRFSFDPTQK
101: LGLDIASCIL TRAPLGQDAE GKPKFVIRPY TPISDPQSKG YFDLLIKVYP EGKMSQHFAS LKPGDVVEVK GPIEKLRYTP NMKKHIGMIA GGTGITPMLQ
201: VIEAILKNPD DKTQISLLYA NVSPDDILLK QKLDILATSH PNLKIFYTVD NPTKNWRGGA GYISKDIVVK GLPGPSDDTL ILVCGPPGMM KAISGEKAKD
301: WTQGEVSGVL KEAGYTEQMV YKF

Best Arabidopsis Sequence Match ( AT5G20080.1 )
(BLAST)

001: MATSFFRRLA RSAPITFPVA FGSQSKSGSG AFRFSTGAIA ALSGGFSYYY LTSGNNLVYL DQAKEETGPK TALNPDKWLE FKLQDTARVS HNTQLFRFSF
101: DPSAELGLHV ASCLLTRAPL GYNAEGKTKY VIRPYTPISD PEAKGYFDLL IKVYPDGKMS QHFASLKPGD VLEVKGPVEK FKYSPNMKKH IGMIAGGSGI
201: TPMLQVIDAI VKNPEDNTQI SLLYANVSPD DILLKQKLDV LQANHPNLKI FYTVDNPTKN WKGGVGYISK DMALKGLPLP TDDTLILVCG PPGMMEHISG
301: GKAPDWSQGE VKGILKELGY TEEMVFKF

Arabidopsis Description

CBR2NADH-cytochrome b5 reductase-like protein [Source:UniProtKB/Swiss-Prot;Acc:P83291]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.