CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion, plastid, cytosol

Predictor Summary:

nucleus 1
plastid 4
cytosol 1
peroxisome 1
mitochondrion 2

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH24006
KRH27971
KRH29310
KRH77487

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G77760.1	KRH24006	AT3G48150.1	21798944
AT1G77760.1	KRH27971	AT3G48150.1	21798944
AT1G77760.1	KRH29310	AT3G48150.1	21798944
AT1G77760.1	KRH77487	AT3G48150.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY26836	Canola	cytosol	59.83	49.19
CDX87505	Canola	cytosol, mitochondrion, peroxisome, plastid	29.91	49.1
KRH16605	Soybean	cytosol	83.19	43.0
CDY68886	Canola	cytosol, mitochondrion	64.85	40.85
KRH16606	Soybean	cytosol	74.24	38.95
KRH18816	Soybean	cytosol, mitochondrion, peroxisome	74.89	38.71
GSMUA_Achr8P23160_001	Banana	mitochondrion	56.11	37.57
KRH53180	Soybean	cytosol, mitochondrion, peroxisome	70.52	36.29
VIT_18s0001g03910.t01	Wine grape	plastid	69.0	34.76
PGSC0003DMT400077648	Potato	cytosol	68.34	34.36
Solyc11g013810.1.1	Tomato	cytosol, nucleus	68.34	34.32
CDX68044	Canola	plastid	64.85	32.64
Bra003670.1-P	Field mustard	plastid	64.85	32.64
CDX79234	Canola	plastid	64.85	32.6
Bra008333.1-P	Field mustard	plastid	64.41	32.56
AT1G37130.1	Thale cress	cytosol	64.41	32.17
TraesCS7A01G078500.1	Wheat	cytosol	60.7	32.1
AT1G77760.1	Thale cress	cytosol	64.19	32.06
KRG89951	Soybean	mitochondrion	9.61	31.88
CDX88386	Canola	cytosol, plastid	61.79	31.66
Bra015656.1-P	Field mustard	cytosol	61.35	31.57
TraesCS7D01G073700.1	Wheat	cytosol, nucleus, peroxisome	60.04	31.57
Os08t0468700-00	Rice	plastid	62.88	31.44
Os08t0468100-01	Rice	plastid	62.88	31.44
EES13985	Sorghum	cytosol, mitochondrion, peroxisome, plastid	63.1	31.38
Os02t0770800-01	Rice	cytosol	60.48	31.16
TraesCS6B01G024900.1	Wheat	cytosol, plastid	61.79	30.96
EES07601	Sorghum	cytosol, mitochondrion, peroxisome	60.26	30.94
TraesCS4A01G376700.1	Wheat	cytosol	60.48	30.92
TraesCS6A01G017500.2	Wheat	cytosol, plastid	61.57	30.85
TraesCS6D01G020700.1	Wheat	cytosol, plastid	61.14	30.63
KRG94933	Soybean	mitochondrion	15.94	30.29
TraesCS6D01G306000.1	Wheat	cytosol, peroxisome, plastid	59.17	30.28
GSMUA_Achr9P15480_001	Banana	cytosol	49.56	30.23
TraesCS6B01G356800.1	Wheat	cytosol, peroxisome, plastid	58.95	30.1
TraesCS6A01G326200.1	Wheat	plastid	58.73	29.92
Zm00001d017025_P001	Maize	cytosol	57.86	29.78
HORVU6Hr1G003300.1	Barley	mitochondrion	61.57	29.65
OQU85223	Sorghum	cytosol	58.52	29.04
KRH64963	Soybean	endoplasmic reticulum	17.03	28.06
GSMUA_Achr3P07200_001	Banana	cytosol	63.97	26.04
Zm00001d031769_P001	Maize	mitochondrion	39.08	26.02
KRH44741	Soybean	endoplasmic reticulum, peroxisome, plastid	16.81	25.93
KRH13184	Soybean	endoplasmic reticulum, peroxisome, plastid	16.81	25.93
Zm00001d052139_P001	Maize	cytosol, mitochondrion, peroxisome	36.24	25.62
Zm00001d049995_P001	Maize	cytosol, mitochondrion, peroxisome, plastid	37.12	25.19
Zm00001d018206_P001	Maize	peroxisome	35.81	24.85
KRH56619	Soybean	mitochondrion	16.38	23.22
KRH03716	Soybean	mitochondrion	15.94	22.88
KRG89948	Soybean	endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	6.99	22.86
GSMUA_Achr5P05820_001	Banana	mitochondrion, nucleus, peroxisome	22.49	22.54
GSMUA_Achr5P05830_001	Banana	cytosol	15.94	20.39

Protein Annotations

Gene3D:2.40.30.10	Gene3D:2.60.40.650	Gene3D:3.10.120.10	Gene3D:3.40.50.80	MapMan:35.1	EMBL:ACUP02008135
InterPro:Cyt_B5-like_heme/steroid-bd	InterPro:Cyt_B5-like_heme/steroid_sf	InterPro:Cyt_B5_heme-BS	InterPro:Flavoprot_Pyr_Nucl_cyt_Rdtase	EnsemblPlantsGene:GLYMA_13G083800	GO:GO:0003674
GO:GO:0003824	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005829	GO:GO:0006809	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009703
GO:GO:0009987	GO:GO:0016491	GO:GO:0020037	GO:GO:0030151	GO:GO:0042128	GO:GO:0046872
GO:GO:0055114	InterPro:IPR001199	InterPro:IPR036400	InterPro:Ig_E-set	UniProt:K7LX30	EnsemblPlants:KRH18814
ProteinID:KRH18814	ProteinID:KRH18814.1	InterPro:MoCF_OxRdtse_dimer	InterPro:Mopterin_OxRdtase_euk	InterPro:NADH-Cyt_B5_reductase	InterPro:OxRdtase_FAD-bd_dom
InterPro:OxRdtase_FAD/NAD-bd	PFAM:PF00173	PFAM:PF00175	PFAM:PF00970	PFAM:PF03404	PRINTS:PR00363
PRINTS:PR00371	PRINTS:PR00406	PRINTS:PR00407	ScanProsite:PS00191	PFscan:PS50255	PANTHER:PTHR19370
PANTHER:PTHR19370:SF182	InterPro:Riboflavin_synthase-like_b-brl	SMART:SM01117	SUPFAM:SSF52343	SUPFAM:SSF55856	SUPFAM:SSF63380
SUPFAM:SSF81296	UniParc:UPI0002966430	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr13:-:19444299..19448036

Molecular Weight (calculated)

52269.9 Da

IEP (calculated)

8.172

GRAVY (calculated)

-0.395

Length

458 amino acids

Sequence
(BLAST)

001: MQEILPINSF TMQYLLKGYA YTGGGRKLTR VEVTLDVRKT WRVCKLDRTE KPNKYGKYCC WCFWSLELEV MNLLGTKEIA GLMNNCWFRV KTNVAKPHKG
101: EIGIVFEHPT QPANQPGGWM VKEKHLEKSL EKLPTLRKSG STQSMNTSSK MFTISEVKKH CTSDSTWIII HGHVYDCTRF LKDHPGGVDS ILINADTDCT
201: EEFDAIHSDK AKQMLQDFRI GDLMSTCRKL ITTPPPPREK ISCKLVSKTS ISHDDQLLGL PVGKHIFLCT TINDKLCMRA YTPTSSVDEV GFSELVIKVY
301: FKACTPSSQT EDSCLNIWTP SPLGHIEYTA RGNFLVHRKQ RFAKRLAMLA NGTGITPIYQ VAQTILKDPE DRTKMHEVYA NKTEDDILLK DEMDVWEKTH
401: CDRFKVWYVV GTAREGWGYS VGFITESITR EHLPETSRDA LAFFDLWTTT YDSIRSAT

Best Arabidopsis Sequence Match ( AT1G37130.1 )
(BLAST)

001: MAASVDNRQY ARLEPGLNGV VRSYKPPVPG RSDSPKAHQN QTTNQTVFLK PAKVHDDDED VSSEDENETH NSNAVYYKEM IRKSNAELEP SVLDPRDEYT
101: ADSWIERNPS MVRLTGKHPF NSEAPLNRLM HHGFITPVPL HYVRNHGHVP KAQWAEWTVE VTGFVKRPMK FTMDQLVSEF AYREFAATLV CAGNRRKEQN
201: MVKKSKGFNW GSAGVSTSVW RGVPLCDVLR RCGIFSRKGG ALNVCFEGSE DLPGGAGTAG SKYGTSIKKE YAMDPSRDII LAYMQNGEYL TPDHGFPVRI
301: IIPGFIGGRM VKWLKRIIVT TKESDNFYHF KDNRVLPSLV DAELADEEGW WYKPEYIINE LNINSVITTP CHEEILPINA FTTQRPYTLK GYAYSGGGKK
401: VTRVEVTVDG GETWNVCALD HQEKPNKYGK FWCWCFWSLE VEVLDLLSAK EIAVRAWDET LNTQPEKMIW NLMGMMNNCW FRVKTNVCKP HKGEIGIVFE
501: HPTLPGNESG GWMAKERHLE KSADAPPSLK KSVSTPFMNT TAKMYSMSEV KKHNSADSCW IIVHGHIYDC TRFLMDHPGG SDSILINAGT DCTEEFEAIH
601: SDKAKKMLED YRIGELITTG YSSDSSSPNN SVHGSSAVFS LLAPIGEATP VRNLALVNPR AKVPVQLVEK TSISHDVRKF RFALPVEDMV LGLPVGKHIF
701: LCATINDKLC LRAYTPSSTV DVVGYFELVV KIYFGGVHPR FPNGGLMSQY LDSLPIGSTL EIKGPLGHVE YLGKGSFTVH GKPKFADKLA MLAGGTGITP
801: VYQIIQAILK DPEDETEMYV IYANRTEEDI LLREELDGWA EQYPDRLKVW YVVESAKEGW AYSTGFISEA IMREHIPDGL DGSALAMACG PPPMIQFAVQ
901: PNLEKMQYNI KEDFLIF

Arabidopsis Description

NIA2Nitrate reductase [NADH] 2 [Source:UniProtKB/Swiss-Prot;Acc:P11035]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.