Factsheet for Bra008333.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

plastid 6
nucleus 1
mitochondrion 2

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra018081.1-P
Bra033769.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G77760.1	Bra018081.1-P	AT3G48150.1	21798944
AT1G77760.1	Bra033769.1-P	AT3G48150.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY68886	Canola	cytosol, mitochondrion	79.58	99.17
Bra003670.1-P	Field mustard	plastid	93.05	92.64
CDY26836	Canola	cytosol	56.29	91.56
AT1G77760.1	Thale cress	cytosol	91.28	90.19
Bra015656.1-P	Field mustard	cytosol	87.09	88.65
VIT_18s0001g03910.t01	Wine grape	plastid	76.49	76.24
KRH18816	Soybean	cytosol, mitochondrion, peroxisome	73.95	75.62
Solyc11g013810.1.1	Tomato	cytosol, nucleus	74.83	74.34
PGSC0003DMT400077648	Potato	cytosol	74.72	74.31
KRH53180	Soybean	cytosol, mitochondrion, peroxisome	72.08	73.37
KRH16605	Soybean	cytosol	69.32	70.88
GSMUA_Achr9P15480_001	Banana	cytosol	57.4	69.24
GSMUA_Achr8P23160_001	Banana	mitochondrion	51.88	68.71
KRH16606	Soybean	cytosol	65.67	68.16
EES13985	Sorghum	cytosol, mitochondrion, peroxisome, plastid	67.11	66.02
Zm00001d049995_P001	Maize	cytosol, mitochondrion, peroxisome, plastid	49.12	65.93
Os02t0770800-01	Rice	cytosol	64.68	65.92
TraesCS6D01G306000.1	Wheat	cytosol, peroxisome, plastid	65.01	65.81
Zm00001d052139_P001	Maize	cytosol, mitochondrion, peroxisome	47.02	65.74
TraesCS7D01G073700.1	Wheat	cytosol, nucleus, peroxisome	63.13	65.67
Os08t0468100-01	Rice	plastid	66.34	65.61
Zm00001d031769_P001	Maize	mitochondrion	49.56	65.26
TraesCS7A01G078500.1	Wheat	cytosol	62.36	65.24
EES07601	Sorghum	cytosol, mitochondrion, peroxisome	64.02	65.02
TraesCS6B01G356800.1	Wheat	cytosol, peroxisome, plastid	64.24	64.88
TraesCS6A01G326200.1	Wheat	plastid	64.35	64.85
Os08t0468700-00	Rice	plastid	65.56	64.85
Zm00001d018206_P001	Maize	peroxisome	47.13	64.7
KRH18814	Soybean	cytosol, mitochondrion, plastid	32.56	64.41
TraesCS6A01G017500.2	Wheat	cytosol, plastid	64.9	64.33
TraesCS6B01G024900.1	Wheat	cytosol, plastid	64.79	64.22
TraesCS6D01G020700.1	Wheat	cytosol, plastid	64.79	64.22
TraesCS4A01G376700.1	Wheat	cytosol	62.69	63.39
HORVU6Hr1G003300.1	Barley	mitochondrion	64.79	61.72
Zm00001d017025_P001	Maize	cytosol	60.49	61.57
OQU85223	Sorghum	cytosol	60.26	59.15
GSMUA_Achr3P07200_001	Banana	cytosol	65.12	52.44
GSMUA_Achr5P05820_001	Banana	mitochondrion, nucleus, peroxisome	21.08	41.79
GSMUA_Achr5P05830_001	Banana	cytosol	15.34	38.83
Bra006419.1-P	Field mustard	endoplasmic reticulum	10.82	34.75
Bra002104.1-P	Field mustard	endoplasmic reticulum, extracellular, golgi, mitochondrion, peroxisome, plasma membrane, plastid, vacuole	10.71	34.52
Bra023636.1-P	Field mustard	endoplasmic reticulum, extracellular, golgi, mitochondrion, peroxisome, plasma membrane, plastid, vacuole	9.49	34.26

Protein Annotations

Gene3D:2.40.30.10	Gene3D:2.60.40.650	MapMan:25.1.4.1	Gene3D:3.10.120.10	Gene3D:3.40.50.80	Gene3D:3.90.420.10
EnsemblPlantsGene:Bra008333	EnsemblPlants:Bra008333.1	EnsemblPlants:Bra008333.1-P	InterPro:Cyt_B5-like_heme/steroid-bd	InterPro:Cyt_B5-like_heme/steroid_sf	InterPro:Cyt_B5_heme-BS
InterPro:Fd_Rdtase_FAD-bd	InterPro:Flavoprot_Pyr_Nucl_cyt_Rdtase	GO:GO:0003674	GO:GO:0003824	GO:GO:0005488	GO:GO:0006809
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016491	GO:GO:0020037
GO:GO:0030151	GO:GO:0042128	GO:GO:0043546	GO:GO:0046872	GO:GO:0050464	GO:GO:0055114
InterPro:IPR001199	InterPro:IPR017927	InterPro:IPR036374	InterPro:IPR036400	InterPro:Ig_E-set	UniProt:M4CVT6
InterPro:MoCF_OxRdtse_dimer	InterPro:Mopterin_OxRdtase_euk	InterPro:NADH-Cyt_B5_reductase	InterPro:Nitr_rd_NADH	InterPro:OxRdtase_FAD-bd_dom	InterPro:OxRdtase_FAD/NAD-bd
InterPro:OxRdtase_Mopterin-bd_dom	InterPro:OxRdtase_Mopterin-bd_sf	InterPro:OxRdtase_Mopterin_BS	PFAM:PF00173	PFAM:PF00174	PFAM:PF00175
PFAM:PF00970	PFAM:PF03404	PIRSF:PIRSF000233	PRINTS:PR00363	PRINTS:PR00371	PRINTS:PR00406
PRINTS:PR00407	ScanProsite:PS00191	ScanProsite:PS00559	PFscan:PS50255	PFscan:PS51384	PANTHER:PTHR19370
PANTHER:PTHR19370:SF182	InterPro:Riboflavin_synthase-like_b-brl	SMART:SM01117	SUPFAM:SSF52343	SUPFAM:SSF55856	SUPFAM:SSF56524
SUPFAM:SSF63380	SUPFAM:SSF81296	UniParc:UPI00025462ED	SEG:seg	:	:

Description

AT1G77760 (E=0.0) NIA1, GNR1, NR1 | NIA1 (NITRATE REDUCTASE 1); nitrate reductase

Coordinates

chrA02:-:14778313..14782014

Molecular Weight (calculated)

101826.0 Da

IEP (calculated)

6.815

GRAVY (calculated)

-0.384

Length

906 amino acids

Sequence
(BLAST)

001: MATSVDNRHY PSLTSAMNAG VVRSFKRPPV PSRSFDSHRH QNVQTVVRGN ENTDGYDSSD DEDESHNRNV SYYMTMVHKS NSDLEPSILD SRDESTADNW
101: IHRNSSMVRL TGKHPFNAEA PLPRLMHHGF ITPVPLHYVR NHGPVPKADW SEWTIEVTGL VKRPVILTME QLISEFPSRE FPVTLVCAGN RRKEQNMVKQ
201: TIGFNWGSAG VSTSLWKGVP LSEILRRCGV YSRRGGALNV CFEGAEDLPG GGGSKYGTSI KKEMAMDPAR DIILAYMQNG ELLTPDHGFP VRIIVPGFIG
301: GRMVKWLKRI IVTPQESDSY YHYKDNRVLP SLVDAELANA EAWWYKPEYI INELNINSVI TTPGHEEILP INAFTTQKPY TLRGYAYSGG GKKVTRVEVT
401: LDGGETWSVC ELDHQEKPNK YGKFWCWCFW SLDVEVLDLL SAKDVAVRAW DESFNTQPDK LIWNLMGMMN NCWFRIRTNV CKPHRGEIGI VFEHPTRPGN
501: QSGGWMAKER QLEISESNPT LKKSVSSPFM NTSSKMYSIS DVRKHNSVES AWIIVHGHVY DCTRFLKDHP GGTDSILINA GTDCTEEFEA IHSDKAKKIL
601: EDYRIGELIT TGYDSSPNVS VHGGSNVAPL LAPIKELSPP KNIALVNPRE KVPVKLIEKT SISHDVRRFR FALPSEDQQL GLPVGKHIFL CANINDKLCL
701: RAYTPTSTVD AVGHIDLVVK VYFKDVHPRF PNGGLMSQHL DSLSIGAVLD IKGPLGHIEY KGKGNFMVNG KPKFAKKLAM LAGGTGITPI YQVIQSILSD
801: PEDETEMYVV YANRTEDDIL VREELEGWAN KHKERLKLWY VVEIAKEGWN YSTGFITEDV LREHVPEGLE GESLALACGP PPMIQFALQP NLEKMGYNVK
901: EDLLIF

Best Arabidopsis Sequence Match ( AT1G77760.1 )
(BLAST)

001: MATSVDNRHY PTMNGVAHAF KPPLVPSPRS FDRHRHQNQT LDVILTETKI VKETEVITTV VDSYDDSSSD DEDESHNRNV PYYKELVKKS NSDLEPSILD
101: PRDESTADSW IQRNSSMLRL TGKHPFNAEA PLPRLMHHGF ITPVPLHYVR NHGAVPKANW SDWSIEITGL VKRPAKFTME ELISEFPSRE FPVTLVCAGN
201: RRKEQNMVKQ TIGFNWGSAG VSTSLWKGIP LSEILRRCGI YSRRGGALNV CFEGAEDLPG GGGSKYGTSI KKEMAMDPAR DIILAYMQNG ELLTPDHGFP
301: VRVIVPGFIG GRMVKWLKRI IVTPQESDSY YHYKDNRVLP SLVDAELANS EAWWYKPEYI INELNINSVI TTPGHAEILP INAFTTQKPY TLKGYAYSGG
401: GKKVTRVEVT LDGGDTWSVC ELDHQEKPNK YGKFWCWCFW SLDVEVLDLL SAKDVAVRAW DESFNTQPDK LIWNLMGMMN NCWFRIRTNV CKPHRGEIGI
501: VFEHPTRPGN QSGGWMAKER QLEISSESNN TLKKSVSSPF MNTASKMYSI SEVRKHNTAD SAWIIVHGHI YDCTRFLKDH PGGTDSILIN AGTDCTEEFE
601: AIHSDKAKKL LEDYRIGELI TTGYDSSPNV SVHGASNFGP LLAPIKELTP QKNIALVNPR EKIPVRLIEK TSISHDVRKF RFALPSEDQQ LGLPVGKHVF
701: VCANINDKLC LRAYTPTSAI DAVGHIDLVV KVYFKDVHPR FPNGGLMSQH LDSLPIGSMI DIKGPLGHIE YKGKGNFLVS GKPKFAKKLA MLAGGTGITP
801: IYQIIQSILS DPEDETEMYV VYANRTEDDI LVREELEGWA SKHKERLKIW YVVEIAKEGW SYSTGFITEA VLREHIPEGL EGESLALACG PPPMIQFALQ
901: PNLEKMGYNV KEDLLIF

Arabidopsis Description

NIA1Nitrate reductase [Source:UniProtKB/TrEMBL;Acc:A0A178WBR8]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.