Factsheet for PGSC0003DMT400077648

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 4
plastid 3
peroxisome 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400063189

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G77760.1	PGSC0003DMT400063189	AT3G48150.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc11g013810.1.1	Tomato	cytosol, nucleus	97.91	97.81
VIT_18s0001g03910.t01	Wine grape	plastid	80.68	80.86
CDY68886	Canola	cytosol, mitochondrion	63.45	79.5
KRH53180	Soybean	cytosol, mitochondrion, peroxisome	75.41	77.19
KRH18816	Soybean	cytosol, mitochondrion, peroxisome	74.97	77.09
AT1G37130.1	Thale cress	cytosol	76.51	76.01
Bra003670.1-P	Field mustard	plastid	75.3	75.38
CDX68044	Canola	plastid	75.19	75.27
CDX79234	Canola	plastid	75.08	75.08
Bra015656.1-P	Field mustard	cytosol	73.22	74.94
Bra008333.1-P	Field mustard	plastid	74.31	74.72
CDX88386	Canola	cytosol, plastid	73.33	74.72
CDX87505	Canola	cytosol, mitochondrion, peroxisome, plastid	22.72	74.19
AT1G77760.1	Thale cress	cytosol	74.09	73.61
KRH16605	Soybean	cytosol	70.58	72.57
GSMUA_Achr9P15480_001	Banana	cytosol	59.28	71.9
CDY26836	Canola	cytosol	43.69	71.45
KRH16606	Soybean	cytosol	67.73	70.68
EES13985	Sorghum	cytosol, mitochondrion, peroxisome, plastid	70.36	69.6
GSMUA_Achr8P23160_001	Banana	mitochondrion	52.25	69.59
Os08t0468100-01	Rice	plastid	69.7	69.32
Zm00001d049995_P001	Maize	cytosol, mitochondrion, peroxisome, plastid	51.04	68.89
TraesCS7A01G078500.1	Wheat	cytosol	65.2	68.59
Zm00001d031769_P001	Maize	mitochondrion	51.7	68.46
Os08t0468700-00	Rice	plastid	68.83	68.45
KRH18814	Soybean	cytosol, mitochondrion, plastid	34.36	68.34
TraesCS7D01G073700.1	Wheat	cytosol, nucleus, peroxisome	65.09	68.08
TraesCS6A01G017500.2	Wheat	cytosol, plastid	68.17	67.94
TraesCS6B01G024900.1	Wheat	cytosol, plastid	68.17	67.94
TraesCS6D01G020700.1	Wheat	cytosol, plastid	68.17	67.94
Zm00001d052139_P001	Maize	cytosol, mitochondrion, peroxisome	47.97	67.44
EES07601	Sorghum	cytosol, mitochondrion, peroxisome	65.31	66.7
TraesCS4A01G376700.1	Wheat	cytosol	65.31	66.41
Zm00001d018206_P001	Maize	peroxisome	48.08	66.36
Os02t0770800-01	Rice	cytosol	64.43	66.03
HORVU6Hr1G003300.1	Barley	mitochondrion	68.83	65.93
TraesCS6D01G306000.1	Wheat	cytosol, peroxisome, plastid	64.54	65.7
TraesCS6B01G356800.1	Wheat	cytosol, peroxisome, plastid	64.11	65.11
TraesCS6A01G326200.1	Wheat	plastid	64.0	64.85
Zm00001d017025_P001	Maize	cytosol	61.36	62.81
OQU85223	Sorghum	cytosol	61.69	60.89
GSMUA_Achr3P07200_001	Banana	cytosol	67.84	54.93
GSMUA_Achr5P05820_001	Banana	mitochondrion, nucleus, peroxisome	23.38	46.61
GSMUA_Achr5P05830_001	Banana	cytosol	16.25	41.34
PGSC0003DMT400072515	Potato	endoplasmic reticulum, extracellular, golgi, mitochondrion, peroxisome, plasma membrane, plastid, vacuole	11.31	37.05

Protein Annotations

Gene3D:2.40.30.10	Gene3D:2.60.40.650	MapMan:25.1.4.1	Gene3D:3.10.120.10	Gene3D:3.40.50.80	Gene3D:3.90.420.10
ProteinID:AAB18985	ProteinID:AAB18985.1	ProteinID:AAB52786	ProteinID:AAB52786.1	EMBL:AB062142	EMBL:AB062143
ProteinID:BAB93533	ProteinID:BAB93533.1	ProteinID:BAB93534	ProteinID:BAB93534.1	InterPro:Cyt_B5-like_heme/steroid-bd	InterPro:Cyt_B5-like_heme/steroid_sf
InterPro:Cyt_B5_heme-BS	InterPro:Fd_Rdtase_FAD-bd	InterPro:Flavoprot_Pyr_Nucl_cyt_Rdtase	GO:GO:0003674	GO:GO:0003824	GO:GO:0005488
GO:GO:0006809	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016491
GO:GO:0020037	GO:GO:0030151	GO:GO:0042128	GO:GO:0043546	GO:GO:0046872	GO:GO:0050464
GO:GO:0055114	InterPro:IPR001199	InterPro:IPR017927	InterPro:IPR036374	InterPro:IPR036400	InterPro:Ig_E-set
UniProt:M1CYP7	InterPro:MoCF_OxRdtse_dimer	InterPro:Mopterin_OxRdtase_euk	InterPro:NADH-Cyt_B5_reductase	InterPro:Nitr_rd_NADH	InterPro:OxRdtase_FAD-bd_dom
InterPro:OxRdtase_FAD/NAD-bd	InterPro:OxRdtase_Mopterin-bd_dom	InterPro:OxRdtase_Mopterin-bd_sf	InterPro:OxRdtase_Mopterin_BS	PFAM:PF00173	PFAM:PF00174
PFAM:PF00175	PFAM:PF00970	PFAM:PF03404	EnsemblPlantsGene:PGSC0003DMG400030212	PGSC:PGSC0003DMG400030212	EnsemblPlants:PGSC0003DMT400077648
PIRSF:PIRSF000233	PRINTS:PR00363	PRINTS:PR00371	PRINTS:PR00406	PRINTS:PR00407	ScanProsite:PS00191
ScanProsite:PS00559	PFscan:PS50255	PFscan:PS51384	PANTHER:PTHR19370	PANTHER:PTHR19370:SF182	InterPro:Riboflavin_synthase-like_b-brl
SMART:SM01117	SUPFAM:SSF52343	SUPFAM:SSF55856	SUPFAM:SSF56524	SUPFAM:SSF63380	SUPFAM:SSF81296
EMBL:U76701	EMBL:U95317	UniParc:UPI000296F6C1	SEG:seg	:	:

Description

Nitrate reductase [Source:PGSC_GENE;Acc:PGSC0003DMG400030212]

Coordinates

chr11:+:9376508..9380925

Molecular Weight (calculated)

102493.0 Da

IEP (calculated)

7.019

GRAVY (calculated)

-0.373

Length

911 amino acids

Sequence
(BLAST)

001: MAASVENRQF THLEPGLSGV GRNFKPRPDS PVRGCNFPPS SNHELPFQKQ QNLPIYLDYS SSEDEDDDDE KNEYVQMIKK GKTELEPSIH DTRDEGTADN
101: WIQRNFSLIR LTGKHPFNSE PPLARLMHHG FITPVPLHYV RNHGPVPKAS WSDWSVEVTG LVKRPMKFTM DQLVNEFPSR ELPVTLVCAG NRRKEQNMVK
201: QTIGFNWGAA AVSTTVWRGV PLRALLKRCG VHSKKKGALN ICFEGSDVLP GGGGSKYGTS IKKEFAMDPS RDIIVAYMQN GEMLSPDHGF PVRMIIPGFI
301: GGRMVKWLKR IVVTTQESES YYHYKDNRVL PPHVDAELAN AEAWWYKPEY IINELNINSV ITTPCHEEIL PINAWTTQRP YTLRGYAYSG GGKKVTRVEV
401: TLDGGETWSV CTLDHPEKPT KYGKYWCWCF WSLEVEVLDL LSAKEIAVRA IDETHNTQPE KLIWNVMGMM NNCWFRVKMN VCKPHKGEIG IVFEHPTQPG
501: NQSGGWMAKE RHLEISAVAP PTLKKSISTP FMNTASKMYS MSEVRKHNSS DSAWIIVHGH IYDASRFLKD HPGGVDSILI NAGTDCTEEF DAIHSDKAKK
601: LLEDFRIGEL ITTGYTSDSS PNTSVHGSSS ISSFLAPIKE LVQTPTRSVA LIPREKIPCK LVDKQSISHD VRKFKFALPS EDQVLGLPVG KHIFLCATVD
701: DKLCMRAYTP TSTVDEVGFF ELVVKIYFKG VHPKFPNGGQ MSQHLDSLPI GAFLDVKGPL GHIEYQGKGN FLVHGKQKFA KKLAMIAGGT GITPVYQVMQ
801: SILKDPEDDT EMYVVYANRT EDDILLKEEL DAWAEQIPDR VKVWYVVQES ITEGWKYSTG FVTEAILREH IPQPSHTTLA LACGPPPMIQ FAINPNLEKM
901: GYDIKEELLV F

Best Arabidopsis Sequence Match ( AT1G37130.1 )
(BLAST)

001: MAASVDNRQY ARLEPGLNGV VRSYKPPVPG RSDSPKAHQN QTTNQTVFLK PAKVHDDDED VSSEDENETH NSNAVYYKEM IRKSNAELEP SVLDPRDEYT
101: ADSWIERNPS MVRLTGKHPF NSEAPLNRLM HHGFITPVPL HYVRNHGHVP KAQWAEWTVE VTGFVKRPMK FTMDQLVSEF AYREFAATLV CAGNRRKEQN
201: MVKKSKGFNW GSAGVSTSVW RGVPLCDVLR RCGIFSRKGG ALNVCFEGSE DLPGGAGTAG SKYGTSIKKE YAMDPSRDII LAYMQNGEYL TPDHGFPVRI
301: IIPGFIGGRM VKWLKRIIVT TKESDNFYHF KDNRVLPSLV DAELADEEGW WYKPEYIINE LNINSVITTP CHEEILPINA FTTQRPYTLK GYAYSGGGKK
401: VTRVEVTVDG GETWNVCALD HQEKPNKYGK FWCWCFWSLE VEVLDLLSAK EIAVRAWDET LNTQPEKMIW NLMGMMNNCW FRVKTNVCKP HKGEIGIVFE
501: HPTLPGNESG GWMAKERHLE KSADAPPSLK KSVSTPFMNT TAKMYSMSEV KKHNSADSCW IIVHGHIYDC TRFLMDHPGG SDSILINAGT DCTEEFEAIH
601: SDKAKKMLED YRIGELITTG YSSDSSSPNN SVHGSSAVFS LLAPIGEATP VRNLALVNPR AKVPVQLVEK TSISHDVRKF RFALPVEDMV LGLPVGKHIF
701: LCATINDKLC LRAYTPSSTV DVVGYFELVV KIYFGGVHPR FPNGGLMSQY LDSLPIGSTL EIKGPLGHVE YLGKGSFTVH GKPKFADKLA MLAGGTGITP
801: VYQIIQAILK DPEDETEMYV IYANRTEEDI LLREELDGWA EQYPDRLKVW YVVESAKEGW AYSTGFISEA IMREHIPDGL DGSALAMACG PPPMIQFAVQ
901: PNLEKMQYNI KEDFLIF

Arabidopsis Description

NIA2Nitrate reductase [NADH] 2 [Source:UniProtKB/Swiss-Prot;Acc:P11035]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.