Factsheet for Os03t0815200-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, extracellular, nucleus, cytosol

Predictor Summary:

cytosol 5
mitochondrion 2

Predictors

GFP

MS/MS

Papers

nucleus: 24133045
extracellular: 26072143
plasma membrane: 27800704

msms PMID: 26072143 doi

B Du, Z Wei, Z Wang, X Wang, X Peng, B Du, R Chen, L Zhu, G He

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China., State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China. Electronic address: gche@whu.edu.cn.

msms PMID: 24133045 doi

DK Jaiswal, D Ray, MK Choudhary, P Subba, A Kumar, J Verma, R Kumar, A Datta, S Chakraborty, N Chakraborty

National Institute of Plant Genome Research, New Delhi, India.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EER90654	Sorghum	cytosol	92.09	92.24
Zm00001d012999_P001	Maize	cytosol	44.11	91.29
TraesCS5B01G481000.1	Wheat	cytosol	90.74	90.74
TraesCS5A01G468400.1	Wheat	cytosol, golgi, unclear	90.74	90.74
TraesCS5D01G481300.1	Wheat	cytosol	90.74	90.74
HORVU5Hr1G111620.1	Barley	cytosol	90.57	90.57
GSMUA_Achr11P...	Banana	cytosol	80.47	86.28
GSMUA_Achr7P21790_001	Banana	cytosol	85.19	85.04
Zm00001d034602_P003	Maize	cytosol	87.88	83.52
VIT_15s0024g00070.t01	Wine grape	cytosol	82.49	82.49
KRH64810	Soybean	cytosol, endoplasmic reticulum, nucleus	82.66	82.38
PGSC0003DMT400060046	Potato	cytosol	82.66	82.24
KRH56478	Soybean	cytosol, nucleus	82.15	82.02
Solyc05g055730.2.1	Tomato	cytosol, unclear	82.15	81.88
PGSC0003DMT400041701	Potato	cytosol	81.31	81.31
Solyc11g008870.1.1	Tomato	extracellular, plastid	81.31	81.31
AT2G44160.1	Thale cress	cytosol	78.45	78.45
CDX79777	Canola	cytosol	78.45	78.32
CDY17879	Canola	cytosol	78.11	78.11
Bra004810.1-P	Field mustard	cytosol	73.06	78.06
CDX98401	Canola	cytosol	77.44	77.7
CDY41193	Canola	cytosol	77.27	77.53
CDX71855	Canola	cytosol	77.27	77.53
Bra007497.1-P	Field mustard	cytosol	77.27	77.53
Bra003397.1-P	Field mustard	cytosol	77.1	77.36
AT3G59970.3	Thale cress	cytosol	76.94	77.2
CDX67820	Canola	cytosol	76.94	77.2
CDY30457	Canola	cytosol	76.94	76.81
CDY23420	Canola	cytosol	76.77	76.64
Bra037664.1-P	Field mustard	cytosol	76.77	76.64
VIT_05s0094g01600.t01	Wine grape	cytosol	8.92	64.63
Zm00001d042247_P001	Maize	extracellular, golgi	12.46	63.25
Zm00001d013002_P001	Maize	golgi, mitochondrion	12.12	61.54

Protein Annotations

KEGG:00670+1.5.1.20	KEGG:00720+1.5.1.20	Gene3D:3.20.20.220	EntrezGene:4334554	MapMan:7.5.7.4	ProteinID:AAR01748.1
ProteinID:AAR89836.1	ProteinID:ABF99537.1	EMBL:AK062082	EMBL:AK099527	EMBL:AK121351	ProteinID:BAF13597.1
ProteinID:BAS87029.1	ProteinID:BAS87030.1	ProteinID:BAS87031.1	ProteinID:EAZ29042.1	InterPro:FAD-linked_oxidoreductase-like	InterPro:Fadh2_euk
GO:GO:0003674	GO:GO:0003824	GO:GO:0004489	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005829	GO:GO:0006555	GO:GO:0006730	GO:GO:0008150	GO:GO:0008152
GO:GO:0009987	GO:GO:0016491	GO:GO:0035999	GO:GO:0055114	EMBL:JX972161	InterPro:Mehydrof_redctse
EnsemblPlantsGene:Os03g0815200	EnsemblPlants:Os03t0815200-01	PFAM:PF02219	PANTHER:PTHR21091	PANTHER:PTHR21091:SF31	UniProt:Q10BJ7
UniProt:Q75HE6	SUPFAM:SSF51730	TIGRFAMs:TIGR00677	UniParc:UPI0000232F2C	RefSeq:XP_015630556.1	:

Description

Similar to Methylenetetrahydrofolate reductase (EC 1.5.1.20). (Os03t0815200-01);Similar to Methylenetetrahydrofolate reductase. (Os03t0815200-02);Similar to Methylenetetrahydrofolate reductase. (Os03t0815200-03);Similar to Methylenetetrahydrofolate reductase (Fragment). (Os03t0815200-04)

Coordinates

chr3:-:34167824..34173328

Molecular Weight (calculated)

66434.4 Da

IEP (calculated)

5.219

GRAVY (calculated)

-0.263

Length

594 amino acids

Sequence
(BLAST)

001: MKVIEKIQEA AADGRTVFSF EYFPPKTEEG LDNLFERMDR MVAHGPNFCD ITWGAGGSTA DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDDALTTIK
101: SNGIQNVLAL RGDPPHGQDK FVQVAGGFAC ALDLVQHIRA KYGDYFGITV AGYPEAHPDA IQSTEGATPE AYSNDLAYLK QKVDAGADLI ITQLFYDTDI
201: FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK IPAEITAALE PIKDNEEAVK AYGIHLGTEM CKKILATGIK TLHLYTLNME KSALGILMNL
301: GLIEESKISR SLPWRPPTNV FRVKEDVRPI FWANRPKSYI SRTLGWDQYP HGRWGDSRNP SYGALTDYQF TRPRGRGKKL QEEWAVPVKS VEDINERFMN
401: FCQGKLTSSP WSELDGLQPE TKIIDDQLVK INQKGFLTIN SQPAVNGERS DSTSVGWGGP GGYVYQKAYL EFFCSKEKLD QLIEKSKAFP SLTYIAVNKD
501: GESFSNIPTN AVNAVTWGVF PGKEIVQPTV VDSASFMVWK DEAFEIWSKG WACLFPEGDS SREILDKVQK SYFLVSLVDN DYINGDLFAA FKEI

Best Arabidopsis Sequence Match ( AT2G44160.1 )
(BLAST)

001: MKVIDKIQSL ADEGKTAFSF EFFPPKTEDG VDNLFERMDR MVAYGPTFCD ITWGAGGSTA DLTLDIASRM QNVVCVESMM HLTCTNMPVE KIDHALETIR
101: SNGIQNVLAL RGDPPHGQDK FVQVEGGFDC ALDLVNHIRS KYGDYFGITV AGYPEAHPDV IGENGLASNE AYQSDLEYLK KKIDAGADLI VTQLFYDTDI
201: FLKFVNDCRQ IGISCPIVPG IMPINNYRGF LRMTGFCKTK IPVEVMAALE PIKDNEEAVK AYGIHLGTEM CKKMLAHGVK SLHLYTLNME KSALAILMNL
301: GMIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWEDFP QGRWGDSRSA SYGALSDHQF SRPRARDKKL QQEWVVPLKS VEDIQEKFKE
401: LCLGNLKSSP WSELDGLQPE TRIINEQLIK VNSKGFLTIN SQPSVNAERS DSPTVGWGGP VGYVYQKAYL EFFCSKEKLD AVVEKCKALP SITYMAVNKG
501: EQWVSNTAQA DVNAVTWGVF PAKEIIQPTI VDPASFNVWK DEAFETWSRS WANLYPEADP SRNLLEEVKN SYYLVSLVEN DYINGDIFAV FADL

Arabidopsis Description

MTHFR2Methylenetetrahydrofolate reductase 2 [Source:UniProtKB/Swiss-Prot;Acc:O80585]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.