Factsheet for Os07t0435100-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, plastid, cytosol

Predictor Summary:

nucleus 2
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704
plastid: 27992503

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os08t0177800-00
Os10t0458700-02

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G64520.1	Os08t0177800-00	AT3G01410.1	21798944
AT1G64520.1	Os10t0458700-02	AT3G01410.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES13525	Sorghum	cytosol	87.64	87.64
Zm00001d024319_P001	Maize	cytosol, extracellular	86.52	86.52
GSMUA_Achr11P...	Banana	cytosol	83.9	83.9
GSMUA_Achr1P21260_001	Banana	cytosol	82.77	82.77
KRH42301	Soybean	nucleus	82.4	82.4
TraesCS4D01G050100.1	Wheat	cytosol	79.03	82.1
VIT_02s0025g02010.t01	Wine grape	cytosol	82.02	82.02
TraesCS7A01G065500.1	Wheat	cytosol, golgi	82.02	82.02
TraesCS4A01G422100.1	Wheat	cytosol	81.65	81.65
TraesCS4B01G049700.1	Wheat	cytosol	81.65	81.65
TraesCS7D01G059900.1	Wheat	cytosol	81.65	81.65
KRH58430	Soybean	nucleus	82.77	80.66
TraesCS6B01G357900.1	Wheat	cytosol	78.65	78.95
CDY69533	Canola	cytosol	78.65	78.65
CDY62022	Canola	extracellular	78.65	78.65
CDY47840	Canola	cytosol	78.65	78.65
Bra040915.1-P	Field mustard	cytosol	78.65	78.65
Bra027705.1-P	Field mustard	cytosol	78.28	78.28
AT1G64520.1	Thale cress	nucleus	78.28	78.28
PGSC0003DMT400031956	Potato	cytosol	77.9	77.9
Solyc08g082700.2.1	Tomato	cytosol, extracellular, nucleus, plastid	76.78	76.78
Zm00001d039483_P001	Maize	cytosol	85.39	70.37
AT5G42040.1	Thale cress	cytosol	60.67	69.53
Zm00001d040137_P001	Maize	cytosol	35.21	62.25
Zm00001d019655_P001	Maize	cytosol	13.86	56.06
TraesCS4D01G012200.1	Wheat	mitochondrion	17.98	48.48
HORVU2Hr1G099580.8	Barley	nucleus	53.18	41.76

Protein Annotations

Gene3D:1.25.40.990	MapMan:19.2.6.2.2.11	InterPro:26S_Psome_Rpn12	EntrezGene:4343069	EMBL:AB037153	EMBL:AK061383
EMBL:AK061408	EMBL:AK101316	ProteinID:BAC84087.1	ProteinID:BAF21414.1	ProteinID:BAT01236.1	InterPro:CSN8_PSD8_EIF3K
ProteinID:EEE67068.1	GO:GO:0000502	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005737	GO:GO:0005829	GO:GO:0005838	GO:GO:0006508	GO:GO:0008150	GO:GO:0008152
GO:GO:0008541	GO:GO:0009056	GO:GO:0009987	GO:GO:0016043	GO:GO:0019538	GO:GO:0043161
GO:GO:0043248	InterPro:IPR000717	EnsemblPlantsGene:Os07g0435100	EnsemblPlants:Os07t0435100-01	InterPro:PCI_dom	PFAM:PF10075
PFscan:PS50250	PANTHER:PTHR12387	UniProt:Q8W423	UniParc:UPI00000AAD10	RefSeq:XP_015648042.1	SEG:seg

Description

19S REGULATORY PARTICLE NON-ATPASE SUBUNIT 12Similar to 26S proteasome subunit RPN12. (Os07t0435100-01)

Coordinates

chr7:-:14527450..14530522

Molecular Weight (calculated)

30773.9 Da

IEP (calculated)

4.723

GRAVY (calculated)

-0.243

Length

267 amino acids

Sequence
(BLAST)

001: MDPKLVEVTQ LFSRFKAAFA RNDFDTCVDL LSQLKVRLTK FPSLPPSFQQ TPNAVEELKI ARDIYEHAVV LSVKIEDQDA FERDFCQLKP YYMDTCGIIP
101: PSPQEYPILG LNLLRLLVQN RIAEFHTELE LLPVTALENP CIKHAVELEQ SFMEGAYNRV LSARQAVPHE TYVYFMDLLA KTVRDELAGC SEKGYDYMSI
201: AEARQVLMFS SDKELHQYIA EEHPEWEVKD GSVFFQKAKE TQPCKEIPSL QVINQTLSYA RELERIV

Best Arabidopsis Sequence Match ( AT1G64520.1 )
(BLAST)

001: MDPQLTEVSQ QFERFKAAFA RKDYNTCSDL LSQLKVLLTK FTSLPPLFEN SPNAAKELTI ARDIYEHAVV LSVKTEDQDA FERDFFQLKP YYVDARNRIP
101: QSPQENLILG LNLLRLLVQN RIAEFHTELE LLSSATLEDP CIKHAVELEQ SFMEGAYNRV LSARQTAPDA TYVYFMDLLA KTIRDEIAGC SEKAYDYVSI
201: SDARQMLLFS SDQELLTYVT DEHPEWEVKE GFVVFQKAKE TAPCKEIPSL QLINQTLSYA RELERIV

Arabidopsis Description

RPN12ARPN12a [Source:UniProtKB/TrEMBL;Acc:A0A178WH62]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.