Factsheet for PGSC0003DMT400026850

Subcellular Localization

min:

: max

Winner_takes_all: extracellular

Predictor Summary:

extracellular 5
endoplasmic reticulum 6
vacuole 7
plasma membrane 5
golgi 6
cytosol 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	extracellular
Any Predictor:	cytosol, secretory
BaCelLo:	secretory
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	golgi, vacuole
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	cytosol
YLoc:	vacuole

cytosol: 22313117
extracellular: 22313117

msms PMID: 22313117 doi

S Lim, K Chisholm, RH Coffin, RD Peters, KI Al-Mughrabi, G Wang-Pruski, DM Pinto

Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400040141
PGSC0003DMT400040142
PGSC0003DMT400040143
PGSC0003DMT400040144
PGSC0003DMT400040206
PGSC0003DMT400040207
PGSC0003DMT400077934

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G02305.1	PGSC0003DMT400040141	AT3G46090.1	17259181
AT1G02305.1	PGSC0003DMT400040142	AT3G46090.1	17259181
AT1G02305.1	PGSC0003DMT400040143	AT3G46090.1	17259181
AT1G02305.1	PGSC0003DMT400040144	AT3G46090.1	17259181
AT1G02305.1	PGSC0003DMT400040206	AT3G46090.1	17259181
AT1G02305.1	PGSC0003DMT400040207	AT3G46090.1	17259181
AT1G02305.1	PGSC0003DMT400077934	AT3G46090.1	17259181

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc02g069110.2.1	Tomato	extracellular, golgi, vacuole	89.47	62.43
Bra030498.1-P	Field mustard	vacuole	72.06	49.86
KRG96636	Soybean	nucleus	71.66	49.72
AT1G02305.1	Thale cress	extracellular, vacuole	72.06	49.17
GSMUA_Achr3P24740_001	Banana	vacuole	70.85	48.75
AT4G01610.1	Thale cress	extracellular, golgi, vacuole	70.45	48.47
GSMUA_Achr8P06100_001	Banana	vacuole	71.66	48.23
KRH68369	Soybean	nucleus	69.64	48.18
Bra036321.1-P	Field mustard	vacuole	68.42	47.21
KRH49642	Soybean	endoplasmic reticulum, extracellular	18.22	46.88
TraesCS4A01G252800.1	Wheat	unclear	64.37	46.22
TraesCS4B01G062500.1	Wheat	extracellular	63.97	46.06
TraesCS4D01G061400.1	Wheat	extracellular	63.97	46.06
AT1G02300.1	Thale cress	extracellular	70.45	45.91
TraesCS4A01G252900.1	Wheat	extracellular	64.78	45.58
HORVU4Hr1G010300.11	Barley	extracellular	65.18	45.48
KXG36303	Sorghum	vacuole	65.18	45.35
TraesCS4A01G252700.1	Wheat	extracellular	63.16	45.22
TraesCS4B01G062400.1	Wheat	extracellular	63.97	44.89
VIT_07s0005g02820.t01	Wine grape	vacuole	69.64	43.88
TraesCS4B01G062600.1	Wheat	vacuole	62.35	42.78
HORVU4Hr1G010360.3	Barley	vacuole	61.94	38.06
HORVU4Hr1G010390.14	Barley	vacuole	64.78	30.53
Zm00001d022297_P001	Maize	cytosol, extracellular, nucleus	7.69	26.03
PGSC0003DMT400048984	Potato	extracellular	29.55	20.51
PGSC0003DMT400071690	Potato	vacuole	27.94	19.22
PGSC0003DMT400079506	Potato	endoplasmic reticulum, extracellular, vacuole	27.13	18.21
PGSC0003DMT400009507	Potato	extracellular	26.72	18.18
PGSC0003DMT400079777	Potato	extracellular	26.72	17.1
Bra039903.1-P	Field mustard	extracellular, golgi, vacuole	13.77	14.78
Os05t0310500-02	Rice	extracellular	6.07	7.77
CDY14798	Canola	extracellular	0.4	0.82

Protein Annotations

Gene3D:1.20.5.170	MapMan:19.4.1.1	Gene3D:3.90.70.10	GO:GO:0003674	GO:GO:0003824	GO:GO:0004197
GO:GO:0006508	GO:GO:0008150	GO:GO:0008152	GO:GO:0008234	GO:GO:0016787	GO:GO:0019538
GO:GO:0050790	UniProt:M1ANS7	PFAM:PF00112	EnsemblPlantsGene:PGSC0003DMG400010362	PGSC:PGSC0003DMG400010362	EnsemblPlants:PGSC0003DMT400026850
PRINTS:PR00705	ScanProsite:PS00139	ScanProsite:PS00639	PANTHER:PTHR12411	PANTHER:PTHR12411:SF516	InterPro:Papain_like_cys_pep_sf
InterPro:Pept_cys_AS	InterPro:Pept_his_AS	InterPro:Peptidase_C1A	InterPro:Peptidase_C1A_C	SMART:SM00645	SUPFAM:SSF54001
UniParc:UPI000295A11F	:	:	:	:	:

Description

Cathepsin B-like cysteine proteinase [Source:PGSC_GENE;Acc:PGSC0003DMG400010362]

Coordinates

chr2:-:28123894..28132918

Molecular Weight (calculated)

27354.3 Da

IEP (calculated)

5.742

GRAVY (calculated)

-0.115

Length

247 amino acids

Sequence
(BLAST)

001: MPFPDSIVVL SFIFQGHCGS CWAFGAVESL SDRFCIHYGL NISLSVNDVI ACCGFYCGNG CDGGSPIRAW HYFIHKGVVS EKCDPYFDNI GCSHPGCEPI
101: YPTPQCNRKC VKENLLWSKS KHFGVNAYMI SSDPYSIMTE VYKNGPVEVA LNVYEDFAHY KSGVYKHVTG EYIGGHAVKL IGWGTSEEGE DYWLIVNSWN
201: RGWGEDGYFK IRRGTNECGI EHSVVAGLPS ARNLNVELGD AVLDASM

Best Arabidopsis Sequence Match ( AT4G01610.1 )
(BLAST)

001: MAVYNTKLCL ASVFLLLGLL LAFDLKGIEA ESLTKQKLDS KILQDEIVKK VNENPNAGWK AAINDRFSNA TVAEFKRLLG VKPTPKKHFL GVPIVSHDPS
101: LKLPKAFDAR TAWPQCTSIG NILDQGHCGS CWAFGAVESL SDRFCIQFGM NISLSVNDLL ACCGFRCGDG CDGGYPIAAW QYFSYSGVVT EECDPYFDNT
201: GCSHPGCEPA YPTPKCSRKC VSDNKLWSES KHYSVSTYTV KSNPQDIMAE VYKNGPVEVS FTVYEDFAHY KSGVYKHITG SNIGGHAVKL IGWGTSSEGE
301: DYWLMANQWN RGWGDDGYFM IRRGTNECGI EDEPVAGLPS SKNVFRVDTG SNDLPVASV

Arabidopsis Description

CATHB3Cathepsin B-like protease 3 [Source:UniProtKB/Swiss-Prot;Acc:Q94K85]
SUBAcon: [golgi,extracellular,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.