CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

extracellular 6
endoplasmic reticulum 5
vacuole 8
plasma membrane 5
golgi 5
cytosol 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, secretory
BaCelLo:	secretory
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	vacuole
Plant-mPloc:	vacuole
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	extracellular
YLoc:	vacuole

nucleus: 21132161
nucleus: 24385303
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 24385303 doi

S Komatsu, S Hiraga, MZ Nouri

National Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, Tsukuba, 305-8518, Japan, skomatsu@affrc.go.jp.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH68369	Soybean	nucleus	96.35	96.08
KRH49642	Soybean	endoplasmic reticulum, extracellular	20.22	75.0
PGSC0003DMT400026850	Potato	extracellular	49.72	71.66
Solyc02g069090.2.1	Tomato	extracellular	69.1	69.89
TraesCS4A01G252800.1	Wheat	unclear	64.89	67.15
TraesCS4B01G062500.1	Wheat	extracellular	64.61	67.06
TraesCS4D01G061400.1	Wheat	extracellular	64.33	66.76
TraesCS4B01G062400.1	Wheat	extracellular	66.01	66.76
HORVU4Hr1G010300.11	Barley	extracellular	66.29	66.67
Solyc02g069110.2.1	Tomato	extracellular, golgi, vacuole	66.29	66.67
TraesCS4A01G252900.1	Wheat	extracellular	65.45	66.38
GSMUA_Achr3P24740_001	Banana	vacuole	66.85	66.3
KXG36303	Sorghum	vacuole	66.01	66.2
GSMUA_Achr8P06100_001	Banana	vacuole	67.7	65.67
Solyc02g069100.2.1	Tomato	plastid	64.89	65.25
TraesCS4A01G252700.1	Wheat	extracellular	63.2	65.22
VIT_07s0005g02820.t01	Wine grape	vacuole	71.07	64.54
TraesCS4B01G062600.1	Wheat	vacuole	62.92	62.22
HORVU4Hr1G010360.3	Barley	vacuole	63.76	56.47
HORVU4Hr1G010390.14	Barley	vacuole	65.45	44.47
Os05t0310500-02	Rice	extracellular	19.94	36.79
Zm00001d022297_P001	Maize	cytosol, extracellular, nucleus	6.18	30.14
KRH13188	Soybean	peroxisome	6.18	24.72
KRH02604	Soybean	vacuole	23.31	23.51
KRH12540	Soybean	extracellular	23.31	23.45
KRH05898	Soybean	endoplasmic reticulum, vacuole	23.6	22.7
KRH17382	Soybean	endoplasmic reticulum	23.31	22.62
KRH61087	Soybean	endoplasmic reticulum	23.03	22.4
KRG98609	Soybean	mitochondrion	18.54	22.15
KRH51767	Soybean	endoplasmic reticulum	22.75	22.13
KRG91982	Soybean	endoplasmic reticulum, golgi, vacuole	21.35	21.71
KRH34798	Soybean	endoplasmic reticulum, golgi	22.75	21.32
KRH29385	Soybean	extracellular	21.63	21.21
KRH24406	Soybean	extracellular	21.63	21.1
KRH37496	Soybean	vacuole	23.6	20.69
KRG99207	Soybean	cytosol, nucleus, plastid	15.17	20.22
KRH49717	Soybean	endoplasmic reticulum	8.71	17.61

Protein Annotations

EntrezGene:100788988	MapMan:19.4.1.1	Gene3D:3.90.70.10	EMBL:ACUP02012162	MEROPS:C01.049	EnsemblPlantsGene:GLYMA_19G223300
GO:GO:0003674	GO:GO:0003824	GO:GO:0004197	GO:GO:0005575	GO:GO:0005576	GO:GO:0005615
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005764	GO:GO:0005773	GO:GO:0006508
GO:GO:0008150	GO:GO:0008152	GO:GO:0008234	GO:GO:0009056	GO:GO:0009987	GO:GO:0016787
GO:GO:0019538	GO:GO:0050790	GO:GO:0051603	UniProt:I1NBK8	EnsemblPlants:KRG96636	ProteinID:KRG96636
ProteinID:KRG96636.1	PFAM:PF00112	PFAM:PF08127	PRINTS:PR00705	ScanProsite:PS00139	ScanProsite:PS00639
PANTHER:PTHR12411	PANTHER:PTHR12411:SF516	InterPro:Papain_like_cys_pep_sf	InterPro:Pept_cys_AS	InterPro:Pept_his_AS	InterPro:Peptidase_C1A
InterPro:Peptidase_C1A_C	InterPro:Propeptide_C1A	SMART:SM00645	SUPFAM:SSF54001	SignalP:SignalP-noTM	UniParc:UPI000233E5F6

Description

hypothetical protein

Coordinates

chr19:-:47559126..47564919

Molecular Weight (calculated)

39445.5 Da

IEP (calculated)

6.227

GRAVY (calculated)

-0.280

Length

356 amino acids

Sequence
(BLAST)

001: MASTLLPLAT FFLVLSASYL QIAGAKAQPL TSLKLNSPIL QESIAKEINE NPEAGWEAAI NPHFSNYTVE QFKRLLGVKP TPKKELRSTP AISHPKSLKL
101: PKNFDARTAW SQCSTIGRIL DQGHCGSCWA FGAVESLSDR FCIHFDVNIS LSVNDLLACC GFLCGSGCDG GYPLYAWQYL AHHGVVTEEC DPYFDQIGCS
201: HPGCEPAYRT PKCVKKCVSG NQVWKKSKHY SVNAYRVSSD PHDIMTEVYK NGPVEVAFTV YEDFAHYKSG VYKHITGYEL GGHAVKLIGW GTTEDGEDYW
301: LLANQWNREW GDDGYFKIRR GTNECGIEED VTAGLPSTKN LVREVTDMDA DAAVSF

Best Arabidopsis Sequence Match ( AT1G02305.1 )
(BLAST)

001: MADNCIRLLH SASVFFCLGL LISSFNLLQG IAAENLSKQK LTSWILQNEI VKEVNENPNA GWKASFNDRF ANATVAEFKR LLGVKPTPKT EFLGVPIVSH
101: DISLKLPKEF DARTAWSQCT SIGRILDQGH CGSCWAFGAV ESLSDRFCIK YNMNVSLSVN DLLACCGFLC GQGCNGGYPI AAWRYFKHHG VVTEECDPYF
201: DNTGCSHPGC EPAYPTPKCA RKCVSGNQLW RESKHYGVSA YKVRSHPDDI MAEVYKNGPV EVAFTVYEDF AHYKSGVYKH ITGTNIGGHA VKLIGWGTSD
301: DGEDYWLLAN QWNRSWGDDG YFKIRRGTNE CGIEHGVVAG LPSDRNVVKG ITTSDDLLVS SF

Arabidopsis Description

CATHB2Cathepsin B-like protease 2 [Source:UniProtKB/Swiss-Prot;Acc:Q93VC9]
SUBAcon: [extracellular,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.