Factsheet for Bra002293.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plastid, nucleus

Predictor Summary:

nucleus 3
plastid 3
cytosol 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus, plastid
Any Predictor:	cytosol, nucleus, plastid
BaCelLo:	nucleus
ChloroP:	plastid
MultiLoc:	cytosol
PProwler:	plastid
TargetP:	plastid
WoLF PSORT:	nucleus
YLoc:	nucleus

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra007702.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G20320.1	Bra007702.1-P	AT3G62800.1	24352449

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDX92469	Canola	nucleus, plastid	98.9	98.84
CDY35811	Canola	nucleus	97.87	97.87
AT5G20320.1	Thale cress	cytosol	81.41	78.5
VIT_11s0149g00120.t01	Wine grape	cytosol	43.45	57.55
KRH20103	Soybean	nucleus	54.97	55.1
KRH03544	Soybean	nucleus	54.78	54.95
Solyc07g005030.2.1	Tomato	cytosol	49.48	52.86
Zm00001d025830_P011	Maize	cytosol, nucleus, plasma membrane	46.37	47.3
GSMUA_Achr9P22460_001	Banana	cytosol, golgi, nucleus	38.57	46.44
Os04t0509300-02	Rice	cytosol	46.62	46.17
TraesCS2A01G364700.2	Wheat	cytosol	45.52	46.03
TraesCS2D01G362700.1	Wheat	nucleus	45.52	45.97
OQU81938	Sorghum	mitochondrion	46.37	45.71
HORVU2Hr1G088270.9	Barley	endoplasmic reticulum	44.55	44.71
TraesCS2B01G383300.2	Wheat	plastid	45.58	43.51
Bra031999.1-P	Field mustard	nucleus	25.11	32.26
Bra012789.1-P	Field mustard	cytosol, nucleus, peroxisome	5.0	28.87
PGSC0003DMT400019213	Potato	cytosol, peroxisome, vacuole	7.01	28.47
Bra039552.1-P	Field mustard	cytosol	4.69	27.21
Bra035784.1-P	Field mustard	cytosol	5.24	25.67
Bra019456.1-P	Field mustard	nucleus	22.36	25.36
Bra033293.1-P	Field mustard	cytosol, nucleus, plastid	27.36	24.32

Protein Annotations

Gene3D:1.10.1520.10	MapMan:16.10.1.3	MapMan:16.10.1.4.2	Gene3D:2.170.260.10	Gene3D:3.30.160.20	Gene3D:3.30.160.380
Gene3D:3.40.50.300	EnsemblPlantsGene:Bra002293	EnsemblPlants:Bra002293.1	EnsemblPlants:Bra002293.1-P	ncoils:Coil	InterPro:DEAD/DEAH_box_helicase_dom
InterPro:Dicer_dimer_sf	InterPro:Dicer_dimerisation_dom	GO:GO:0000003	GO:GO:0000166	GO:GO:0003674	GO:GO:0003676
GO:GO:0003723	GO:GO:0003824	GO:GO:0004518	GO:GO:0004525	GO:GO:0005488	GO:GO:0005515
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0006139
GO:GO:0006259	GO:GO:0006353	GO:GO:0006396	GO:GO:0006950	GO:GO:0007275	GO:GO:0008150
GO:GO:0008152	GO:GO:0009056	GO:GO:0009058	GO:GO:0009605	GO:GO:0009607	GO:GO:0009616
GO:GO:0009791	GO:GO:0009944	GO:GO:0009987	GO:GO:0010050	GO:GO:0010216	GO:GO:0010267
GO:GO:0010492	GO:GO:0010599	GO:GO:0016075	GO:GO:0016787	GO:GO:0016891	GO:GO:0040029
GO:GO:0048608	GO:GO:0051214	GO:GO:0051607	GO:GO:0090501	GO:GO:0090502	InterPro:Helicase_ATP-bd
InterPro:Helicase_C	InterPro:IPR000999	InterPro:IPR001650	InterPro:IPR003100	InterPro:IPR005034	InterPro:IPR014001
InterPro:IPR014720	InterPro:IPR036389	InterPro:IPR038248	UniProt:M4CDL2	HAMAP:MF_00104	InterPro:P-loop_NTPase
InterPro:PAZ_dom	PFAM:PF00035	PFAM:PF00270	PFAM:PF00271	PFAM:PF00636	PFAM:PF03368
PFAM:PF14709	ScanProsite:PS00517	PFscan:PS50137	PFscan:PS50142	PFscan:PS50821	PFscan:PS51192
PFscan:PS51194	PFscan:PS51327	PANTHER:PTHR14950	PANTHER:PTHR14950:SF15	InterPro:RNase_III	InterPro:RNase_III_dom
InterPro:RNase_III_sf	SMART:SM00358	SMART:SM00487	SMART:SM00490	SMART:SM00535	SMART:SM00949
SUPFAM:SSF52540	SUPFAM:SSF54768	SUPFAM:SSF69065	UniParc:UPI0002544DDA	InterPro:dsRBD_dom	SEG:seg

Description

AT5G20320 (E=0.0) DCL4, ATDCL4 | DCL4 (DICER-LIKE 4); RNA binding / protein binding / ribonuclease III

Coordinates

chrA10:+:10446633..10455677

Molecular Weight (calculated)

184749.0 Da

IEP (calculated)

6.757

GRAVY (calculated)

-0.200

Length

1641 amino acids

Sequence
(BLAST)

0001: MPDREIGDDA SDTNLSSSFS SFASSSSSFS SSYSAAGAWT DDHSAKMEKD PRKIARRYQL ELCEKAVEEN VIVYLGTGCG KTHIAVMVIY ELGPLILSPR
0101: KSVCIFLAPT VALVEQQALV IAKSINFKVA THCGGNRTVT THSDWEREVS ENEVLVMTPQ ILLHNLQHCF IRMEWISLLI LDECHHAQEQ SNHPYAQILK
0201: VFYKTEGVKG PRIFGMTASP VVGKGSFQSE NLSKSINSLE NLLNAKVYSV ESNVQLDGFV SSPIVKVYYY QTAGTEASQS TNIYENMLED IKQRCLTSLK
0301: QQIDNHQTQV LLNMKKLLKR THDNLIYSLV NLGLWGAIQA ARIQLNTDRN VHQEPLEENN KSKICITYLS LAAEVLSSKV AKDENASELI SLPALKEPFF
0401: SRKLLQLIKI LSAFRIEPHM KCIIFVNRIV TARTLSCILN SLKLLESWKS DFLVGLSSGV KSMSRKSMKT ILERFQSKEL NLLVATKVGE EGLDIQTCCL
0501: VIRFDLPETV TSFIQSRGRA RMPKSEYAFL VDRGSEKEMD LIENFKVNED RMNLEITSRT SEETCPRLDD EVYRVHETGA CISGGSSISL LYKYCSRLPH
0601: DEFFQPKPEF QFKPVDEFGG TICRITLPAN APISEIVSSL LPSIEAAKKN ACLKAVYKLH SLGVLNNFLL PDSNEETEDE LSDEEFDFDK VEGEACSRGE
0701: LYEMIVPDLF KQKWDPSKSC VNLQSYYIRF VPHPADRIYK KFGLFMKSPL PIEAETMDFD LHLAHQRSVS VKIFPKGDAN FDNDEIRLAE RFQEVALKII
0801: FERRELITEF VPLGLQDSFR TSKSTFYLLL PINLDASESV LFVDWATIRS CLSSPIFKAP SGLVEDMDPP VGSHLKLANG CWSIDDVKNS VVFATHKKQF
0901: YFVSDICHGR NGFSAIRKSI TETHLESIYN SYGVKLKHPL QPLLRLKPLC YVRNLLHNRK RENLEPNELE EYFIEIPPEL SQLKIKGLSK DIGSSLSLLP
1001: SVMHRMENLL VAIELKHMLS ASIPEIAEVS GHRVLEALTT EKCQERFSLE RHAVLGDAFL KFAVSRHLFL HHDRLDEGEL TRRRSNAVNN SNLFRLATRR
1101: NLQVYIRDQA FDPTQFFALG HPCRVTCDEV AMKEVHSLDK VPGLLESSTG EIRCSKGHHW LHKKTIADVV EALVGAFLVD SGFKGAIEFL KWIGINVDFE
1201: SLQVRDACVA SKRYMPLTTC FDLAALESLL GYKFLHKGLL LQAFIHPSYN RHGGGCYQRL EFLGDAVLDY LMTSYFFSVF PKLKPGQLTD LRSLSVNNKA
1301: LANVAVSFSL QRFLFCDSTY LHDAIKDYTN FVTASPLASG PSEGPKCPKV LGDLIESFLG ALFLDCGFDL NHVWRIMLSF LDPVKNLSNL QLSPVKELLE
1401: YCQSYKWDQE ISATKKDGAF SVELKVTKKS SCLTASATGR NKKESTKKAA QLMLTNLKAH GHIKTSNPLE DVLKNSIRNE AKLIGYDEEP IDVVDRDGLD
1501: VENLNIQDSF EEDPVTESKT SETSSSYIIR RVLTNAPPSE KEESLPQKTI KDAGGSIIKT AKSLLRETCV ANCWKTPEFV CCEEGPAHLK TFSYKVILEV
1601: VDAPNMTLEC YGEAKPTKKS ASEQAAQAAL WCLEHTGFLR R

Best Arabidopsis Sequence Match ( AT5G20320.1 )
(BLAST)

0001: MRDEVDLSLT IPSKLLGKRD REQKNCEEEK NKNKKAKKQQ KDPILLHTSA ATHKFLPPPL TMPYSEIGDD LRSLDFDHAD VSSDLHLTSS SSVSSFSSSS
0101: SSLFSAAGTD DPSPKMEKDP RKIARRYQVE LCKKATEENV IVYLGTGCGK THIAVMLIYE LGHLVLSPKK SVCIFLAPTV ALVEQQAKVI ADSVNFKVAI
0201: HCGGKRIVKS HSEWEREIAA NEVLVMTPQI LLHNLQHCFI KMECISLLIF DECHHAQQQS NHPYAEIMKV FYKSESLQRP RIFGMTASPV VGKGSFQSEN
0301: LSKSINSLEN LLNAKVYSVE SNVQLDGFVS SPLVKVYYYR SALSDASQST IRYENMLEDI KQRCLASLKL LIDTHQTQTL LSMKRLLKRS HDNLIYTLLN
0401: LGLWGAIQAA KIQLNSDHNV QDEPVGKNPK SKICDTYLSM AAEALSSGVA KDENASDLLS LAALKEPLFS RKLVQLIKIL SVFRLEPHMK CIIFVNRIVT
0501: ARTLSCILNN LELLRSWKSD FLVGLSSGLK SMSRRSMETI LKRFQSKELN LLVATKVGEE GLDIQTCCLV IRYDLPETVT SFIQSRGRAR MPQSEYAFLV
0601: DSGNEKEMDL IENFKVNEDR MNLEITYRSS EETCPRLDEE LYKVHETGAC ISGGSSISLL YKYCSRLPHD EFFQPKPEFQ FKPVDEFGGT ICRITLPANA
0701: PISEIESSLL PSTEAAKKDA CLKAVHELHN LGVLNDFLLP DSKDEIEDEL SDDEFDFDNI KGEGCSRGDL YEMRVPVLFK QKWDPSTSCV NLHSYYIMFV
0801: PHPADRIYKK FGFFMKSPLP VEAETMDIDL HLAHQRSVSV KIFPSGVTEF DNDEIRLAEL FQEIALKVLF ERGELIPDFV PLELQDSSRT SKSTFYLLLP
0901: LCLHDGESVI SVDWVTIRNC LSSPIFKTPS VLVEDIFPPS GSHLKLANGC WNIDDVKNSL VFTTYSKQFY FVADICHGRN GFSPVKESST KSHVESIYKL
1001: YGVELKHPAQ PLLRVKPLCH VRNLLHNRMQ TNLEPQELDE YFIEIPPELS HLKIKGLSKD IGSSLSLLPS IMHRMENLLV AIELKHVLSA SIPEIAEVSG
1101: HRVLEALTTE KCHERLSLER LEVLGDAFLK FAVSRHLFLH HDSLDEGELT RRRSNVVNNS NLCRLAIKKN LQVYIRDQAL DPTQFFAFGH PCRVTCDEVA
1201: SKEVHSLNRD LGILESNTGE IRCSKGHHWL YKKTIADVVE ALVGAFLVDS GFKGAVKFLK WIGVNVDFES LQVQDACIAS RRYLPLTTRN NLETLENQLD
1301: YKFLHKGLLV QAFIHPSYNR HGGGCYQRLE FLGDAVLDYL MTSYFFTVFP KLKPGQLTDL RSLSVNNEAL ANVAVSFSLK RFLFCESIYL HEVIEDYTNF
1401: LASSPLASGQ SEGPRCPKVL GDLVESCLGA LFLDCGFNLN HVWTMMLSFL DPVKNLSNLQ ISPIKELIEL CQSYKWDREI SATKKDGAFT VELKVTKNGC
1501: CLTVSATGRN KREGTKKAAQ LMITNLKAHE NITTSHPLED VLKNGIRNEA KLIGYNEDPI DVVDLVGLDV ENLNILETFG GNSERSSSYV IRRGLPQAPS
1601: KTEDRLPQKA IIKAGGPSSK TAKSLLHETC VANCWKPPHF ECCEEEGPGH LKSFVYKVIL EVEDAPNMTL ECYGEARATK KGAAEHAAQA AIWCLKHSGF
1701: LC

Arabidopsis Description

DCL4Dicer-like protein 4 [Source:UniProtKB/Swiss-Prot;Acc:P84634]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.