Factsheet for Bra035745.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plastid, cytosol

Predictor Summary:

nucleus 2
plastid 3
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra009564.1-P
Bra027674.1-P
Bra035745.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G20780.1	Bra027674.1-P	AT1G63990.1	11410368
AT3G20780.1	Bra035745.1-P	AT3G20780.1	16339310
AT3G20780.1	Bra009564.1-P	AT5G02820.1	11410368
AT3G20780.1	Bra009564.1-P	AT5G02820.1	16339310

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY19605	Canola	cytosol, plastid	98.41	99.56
CDY06380	Canola	cytosol	95.96	99.55
AT3G20780.1	Thale cress	cytosol	91.34	94.48
VIT_06s0004g01530.t01	Wine grape	nucleus	78.21	80.53
KRH23181	Soybean	nucleus	77.92	80.24
Solyc01g014490.2.1	Tomato	nucleus	75.47	77.83
PGSC0003DMT400076882	Potato	nucleus	34.34	77.78
PGSC0003DMT400031535	Potato	cytosol	9.67	72.83
PGSC0003DMT400049158	Potato	cytosol	5.34	72.55
TraesCS5D01G178400.1	Wheat	cytosol, plastid	71.86	72.17
TraesCS5A01G173900.1	Wheat	cytosol	71.86	72.17
PGSC0003DMT400076881	Potato	cytosol	42.28	71.46
EER96475	Sorghum	nucleus	71.0	71.1
Os09t0279600-01	Rice	nucleus	71.14	70.83
GSMUA_Achr1P09300_001	Banana	golgi, mitochondrion	71.43	70.41
HORVU5Hr1G053310.1	Barley	cytosol	71.72	69.32
TraesCS5B01G171400.1	Wheat	nucleus	72.01	67.71
Zm00001d019950_P001	Maize	nucleus	62.77	67.44
PGSC0003DMT400068945	Potato	cytosol, nucleus	7.22	61.73
Solyc11g028300.1.1	Tomato	cytosol	9.52	44.3
Solyc11g028340.1.1	Tomato	mitochondrion	3.9	42.19
Solyc11g028350.1.1	Tomato	mitochondrion	3.9	36.99
PGSC0003DMT400043995	Potato	mitochondrion	9.67	30.04
Solyc12g036030.1.1	Tomato	cytosol	3.75	27.08
Solyc11g028330.1.1	Tomato	cytosol	4.18	23.39

Protein Annotations

Gene3D:1.10.8.50	Gene3D:3.30.230.10	MapMan:35.1	EnsemblPlantsGene:Bra035745	EnsemblPlants:Bra035745.1	EnsemblPlants:Bra035745.1-P
ncoils:Coil	GO:GO:0000166	GO:GO:0000902	GO:GO:0003674	GO:GO:0003676	GO:GO:0003677
GO:GO:0003824	GO:GO:0003916	GO:GO:0003918	GO:GO:0005488	GO:GO:0005515	GO:GO:0005524
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0006139	GO:GO:0006259
GO:GO:0006265	GO:GO:0007049	GO:GO:0007275	GO:GO:0007389	GO:GO:0008150	GO:GO:0008152
GO:GO:0009058	GO:GO:0009330	GO:GO:0009653	GO:GO:0009719	GO:GO:0009741	GO:GO:0009987
GO:GO:0010026	GO:GO:0016043	GO:GO:0016787	GO:GO:0016853	GO:GO:0030154	GO:GO:0042023
GO:GO:0042802	GO:GO:0042803	GO:GO:0061505	InterPro:HATPase_C	InterPro:HATPase_C_sf	InterPro:IPR014721
InterPro:IPR036890	UniProt:M4F3U5	HAMAP:MF_00322	PFAM:PD013790	PFAM:PF02518	PFAM:PF09239
PANTHER:PTHR10871	PANTHER:PTHR10871:SF4	InterPro:Ribosomal_S5_D2-typ_fold	InterPro:Ribosomal_S5_D2-typ_fold_subgr	SUPFAM:SSF54211	SUPFAM:SSF55874
InterPro:TopoVI_B	InterPro:TopoVI_B_transducer	UniParc:UPI00025479FF	SEG:seg	:	:

Description

AT3G20780 (E=0.0) ATTOP6B, BIN3, HYP6, RHL3 | ATTOP6B (topoisomerase 6 subunit B); DNA topoisomerase (ATP-hydrolyzing)/ identical protein binding / protein binding

Coordinates

chrA05:+:16895926..16900257

Molecular Weight (calculated)

78303.4 Da

IEP (calculated)

9.826

GRAVY (calculated)

-0.467

Length

693 amino acids

Sequence
(BLAST)

001: MAGGDSPAET KKGSSKTSKN SKDSSKESTL KQKSPAEFFA ENKNIAGFDN PGKSLYTTVR ELVENALDSS ESISELPEVE VTIEEIGKSK FNSMIGLIDR
101: ERVDTKLYDD YETDKAREKR LVKEARASEI QAKNLASGKK NKEPGVSKVL KARGEASYYK VTCKDNGKGM PHDDIPNMFG RDLRIVLLTV LSGTKYGLKQ
201: TRGKFGLGAK MALIWSKMST GLPIEISSSM KSQNYVTFCR LDIDIHRNIP HIHLHEKKAN KEKWHGAEIQ VVIEGNWTTY RSKILHYMRQ MAVITPYAQF
301: LFRFISETPE KNVTVKFTRR TDVMPPIPIE TKHHPSSVDL LLIKRLVTDT SKKTLLQFLQ NEFVNINKNL AARLIGEMGP DFSPGMAVKS VTSQQMVRIH
401: QLFRQAKFDD PSGDCLSPAG EYNLRLGIIK ELHPDMVATH SGSAQVFEGH PFIVEAGVSV GGRDVKQASH ILLLFIHGIN IFRFANRIPL LFEQGGDVVT
501: RTALKRINWN SYKINQTQDK IGVFVSIVST KIPFKGTGKE YIGDDISEIA TAVKSAIQQC CIQLKSKIVK RLQAREQQER KRSLSRYIPD ATGAVYEVLK
601: QMTEEHTKKR KRYKEEETAM LEKVSKLIIT KETLKEKLAE HVEQVDYEMA LEYATQSGVS EEPKENIYLQ QLNPNHSNFI DLHSPTFVFR LIL

Best Arabidopsis Sequence Match ( AT3G20780.1 )
(BLAST)

001: MAGDDLVETK KGSSKNSKDS NESKLKQKSP AEFFAENKNI AGFDNPGKSL YTTVRELVEN ALDSAESISE LPEVEVTIEE IVKSKFNSMI GLIDRERVDT
101: QLYDDYETEK ARGKRLAKEA RASEIQAKNL ASGKKNKEPG VSKVLKARGE ASYYKVTCKD NGKGMPHDDI PNMFGRVLSG TKYGLKQTRG KFGLGAKMAL
201: IWSKMSTGLP IEISSSMKSQ NYVTFCRLDI DIHRNIPHIH LHEKKGNKEK WHGAEIQVVI EGNWTTYRSK ILHYMRQMAV ITPYAQFLFR FISETPEKNV
301: TIKFTRRTDV MPPIPIETKH HPSSVDLLLI KRLITDTSKK TLLQFLQNEF VNINKTLAAR LIGEMGPDFG PSMAVKSVTS QQMVRIHQLF RQAKFDDPSG
401: DCLSPAGEYN LRLGIIKELH PDMVATYSGS AQVFEGHPFI VEAGVSLGGR DVKQGINIFR FANRIPLLFE QGADVVTRTA LKRINWNSYK INQTQDKIGV
501: FVSIVSTKIP FKGTGKEYIG DDISEIATAV KSAIQQCCIQ LKSKIVKRLQ AREQQERKRS LSRYIPDATG AVYEVLKQMT EEHKTKRKRY GEEDIVMLDK
601: VSKQIITKET LKEKLAEHVE QVDYEMALEY ATQSGVSEEP RENIYLQHLD PNKSNFIDLH SPTFVFRLML

Arabidopsis Description

TOP6BDNA topoisomerase 6 subunit B [Source:UniProtKB/Swiss-Prot;Acc:Q9C5V6]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.