CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

extracellular 6
endoplasmic reticulum 5
vacuole 7
plasma membrane 5
golgi 5

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	vacuole
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	vacuole
YLoc:	extracellular

cytosol: 16847873
cytosol: 19562800

msms PMID: 19562800 doi

P Jolivet, C Boulard, A Bellamy, C Larré, M Barre, H Rogniaux, S d'Andréa, T Chardot, N Nesi

NRA, UMR 206, Chimie Biologique, Thiverval-Grignon, France. jolivet@grignon.inra.fr

msms PMID: 16847873 doi

V Katavic, GK Agrawal, M Hajduch, SL Harris, JJ Thelen

University of Missouri-Columbia, Department of Biochemistry, Columbia 65211, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
CDX74635
CDX84933
CDX95198
CDX95199
CDY03907
CDY15722
CDY27605
CDY42355
CDY47297

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G27140.1	CDX84933	AT1G27300.1	21798944
AT4G27140.1	CDY03907	AT1G27300.1	21798944
AT4G27140.1	CDY15722	AT1G27300.1	21798944
AT4G27140.1	CDY27605	AT1G27300.1	21798944
AT4G27140.1	CDX95198	AT5G42220.1	21798944
AT4G27140.1	CDX95199	AT5G42220.1	21798944
AT4G27140.1	CDY42355	AT5G42220.1	21798944
AT4G27160.1	CDX74635	AT5G35750.1	18642946
AT4G27160.1	CDY47297	AT5G35750.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDX89275	Canola	vacuole	98.26	98.26
CDY29280	Canola	vacuole	97.09	96.53
CDY29278	Canola	vacuole	96.51	96.51
CDX89271	Canola	vacuole	95.93	95.93
CDY29281	Canola	vacuole	95.35	94.8
CDY32264	Canola	cytosol	97.09	93.3
CDX89270	Canola	vacuole	68.02	92.86
CDY61462	Canola	vacuole	96.51	92.22
CDX70800	Canola	vacuole	91.86	89.77
Bra026374.1-P	Field mustard	vacuole	99.42	88.14
CDY29279	Canola	vacuole	90.12	86.11
CDY51244	Canola	cytosol	92.44	85.48
CDY46662	Canola	vacuole	74.42	73.99
AT4G27140.1	Thale cress	extracellular, vacuole	65.12	68.29
AT4G27160.1	Thale cress	extracellular, vacuole	61.63	64.63
AT4G27170.1	Thale cress	extracellular, vacuole	61.63	63.86
AT4G27150.1	Thale cress	extracellular	61.63	62.35
CDY46659	Canola	cytosol	54.07	58.86
CDX92819	Canola	endoplasmic reticulum, extracellular	53.49	58.23
CDY14281	Canola	cytosol, nucleus, plastid	29.65	42.5
CDX97380	Canola	cytosol	29.07	41.67

Protein Annotations

EnsemblPlants:CDX89273	EnsemblPlantsGene:GSBRNA2T00149486001	Gene3D:1.10.110.10	GO:A0A078DPY3	GO:GO:0003674	GO:GO:0045735
InterPro:Bifun_inhib/LTP/seed_sf	InterPro:Bifunc_inhib/LTP/seed_store	InterPro:Napin	PANTHER:PTHR35496	PFAM:PF00234	PRINTS:PR00496
ProteinID:CDX89273	ProteinID:CDX89273.1	SEG:seg	SignalP:SignalP-noTM	SMART:SM00499	SUPFAM:SSF47699
TMHMM:TMhelix	UniParc:UPI0004EDBEA3	MapMan:35.1	:	:	:

Description

BnaA01g16200D

Coordinates

chrLK031858:-:671794..672459

Molecular Weight (calculated)

19379.2 Da

IEP (calculated)

6.980

GRAVY (calculated)

-0.467

Length

172 amino acids

Sequence
(BLAST)

001: MANKLFLVSA TLAFFFLLTN ASIYRTVVEV DEDDATNPAG PFRIPKCRKE FQQAQHLKAC QQWLHKQAMQ SGSGPSWTLD GEFDFEDDME NPQGPQQRPP
101: LLQQCCNELH QEEPLCVCPT LKGASKAVKQ QGQQMQHVIS RIYQTATHLP KVCNIPQVSV CPFQKTMPGP SY

Best Arabidopsis Sequence Match ( AT4G27140.1 )
(BLAST)

001: MANKLFLVCA ALALCFLLTN ASIYRTVVEF EEDDATNPIG PKMRKCRKEF QKEQHLRACQ QLMLQQARQG RSDEFDFEDD MENPQGQQQE QQLFQQCCNE
101: LRQEEPDCVC PTLKQAAKAV RLQGQHQPMQ VRKIYQTAKH LPNVCDIPQV DVCPFNIPSF PSFY

Arabidopsis Description

AT2S12S seed storage protein 1 [Source:UniProtKB/Swiss-Prot;Acc:P15457]
SUBAcon: [extracellular,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.